Genomic Insights and Comparative Genomics of Bacillus Species Having Diverse Mechanisms of Biocontrol Against Fungal Phytopathogens

Rajkumari, Jina; Pandey, Piyush

doi:10.1007/978-3-030-15175-1_12

Cited by 2 publications

(3 citation statements)

References 99 publications

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“…In Pseudomonas sp. and similarly in B. subtilis strain E1R-j, endophytic microflora from wheat (roots) revealed high fungistatic activity and thereby reduced the disease intensity (90.97%) under pot house conditions [75]. The endophytic microfloral formulations have been reported to exhibit higher blight disease containment.…”

Section: Discussionmentioning

confidence: 93%

“…[74]. The wilt prevalence in eggplant to an extent of more than 70% was reported to be contained by the formulations of Pseudomonas sp., Enterobacter sp., and Bacillus sp., and it was found that most of these isolates produced an antibiotic DAPG under in vitro conditions [75]. The naturally-occurring bacterial endophytes that could confer a prominent disease resistance to plants are very low in number and such populations have to be raised to a specific level in order to encourage a contrasting check in the proliferation of phytopathogens [76].…”

Section: Discussionmentioning

confidence: 99%

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Bacterial Endophyte Community Dynamics in Apple (Malus domestica Borkh.) Germplasm and Their Evaluation for Scab Management Strategies

Padder

Mansoor

Bhat

et al. 2021

JoF

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The large genetic evolution due to the sexual reproduction-mediated gene assortments and propensities has made Venturia inaequalis (causing apple scab) unique with respect to its management strategies. The resistance in apple germplasm against the scab, being controlled for by more than fifteen genes, has limited gene alteration-based investigations. Therefore, a biological approach of bacterial endophyte community dynamics was envisioned across the apple germplasm in context to the fungistatic behavior against V. inaequalis. A total of 155 colonies of bacterial endophytes were isolated from various plant parts of the apple, comprising 19 varieties, and after screening for antifungal behavior followed by morphological, ARDRA, and sequence analysis, a total of 71 isolates were selected for this study. The alpha diversity indices were seen to fluctuate greatly among the isolation samples in context to microflora with antifungal behavior. As all the isolates were screened for the presence of various metabolites and some relevant genes that directly or indirectly influence the fungistatic behavior of the isolated microflora, a huge variation among the isolated microflora was observed. The outstanding isolates showing highest percentage growth inhibition of V. inaequalis were exploited to raise a bio-formulation, which was tested against the scab prevalence in eight apple varieties under controlled growth conditions. The formulation at all the concentrations caused considerable reductions in both the disease severity and disease incidence in all the tested apple varieties. Red Delicious being most important cultivar of the northwestern Himalayas was further investigated for its biochemical behavior in formulation and the investigation revealed different levels of enzyme production, chlorophyll, and sugars against the non-inoculated control.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Bacterial Endophyte Community Dynamics in Apple (Malus domestica Borkh.) Germplasm and Their Evaluation for Scab Management Strategies

Padder

Mansoor

Bhat

et al. 2021

JoF

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“…Currently, a number of registered products and those in the development stage are derived from Bacillus species. Reported biocontrol agents (BCAs) from Bacillus species such as Bacillus subtilis , Bacillus pumilis , and Bacillus thuriengiensis are characterized by their antagonist activity and manifest fungistatic and fungicidal effects ( Rajkumari and Pandey, 2019 ). Bacillus spp.…”

Section: Introductionmentioning

confidence: 99%

In vitro assessment of Bacillus subtilis FJ3 affirms its biocontrol and plant growth promoting potential

et al. 2023

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Bacillus species and their metabolites have potential alternative uses as chemical pesticides that can limit the growth of potential plant pathogens and enhance crop productivity. The aim of this study was to investigate the potential of Bacillus subtilis FJ3 for promoting plant growth and controlling fungal plant pathogens. The study evaluated the ability of the strain to promote plant growth in vitro by characterizing its growth-promoting traits, which included the production of hydrolytic enzymes, indole acetic acid, siderophores, biofilm formation, and phosphate solubilization. Polymerase Chain Reaction (PCR) testing revealed that strain FJ3 has the potential to produce lipopeptides such as fengycin, surfactin, mycosubtilin, and pilpastatin. Through in vitro antagonism testing it was demonstrated that strain FJ3 is able to inhibit Fusarium oxysporum by 52% compared to the untreated control and was antagonistic against Aspergillus flavus, Aspergillus niger, and Rhizopus oryzae using a dual method. The minimum inhibitory concentration of Bacillus crude extract resulted in a 92%, 90%, 81.5%, and 56% growth inhibition of Fusarium oxysporum, A. niger, A. flavus, and Rhizopus oryzae, respectively. In FT-IR and GC-MS analysis of crude LPs extract, the transmission and mass spectrum confirmed the existence of aforesaid lipopeptides containing β-fatty acids with chain lengths ranging from C14 to C21 in which the majority were saturated fatty acids. Greenhouse experimentation revealed that Bacillus strain FJ3 and its metabolites significantly diminished the disease incidence with an average reduction of 31.56%. In sterilized soil, FJ3 and its metabolites caused 24.01% and 10.46% growth promotion, respectively, in chickpea. The results demonstrated that Bacillus strain FJ3 has broad-spectrum antifungal and plant growth-promoting applications and could be a promising candidate for development into a commercialized biobased product for use in sustainable agriculture practice.

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Genomic Insights and Comparative Genomics of Bacillus Species Having Diverse Mechanisms of Biocontrol Against Fungal Phytopathogens

Cited by 2 publications

References 99 publications

Bacterial Endophyte Community Dynamics in Apple (Malus domestica Borkh.) Germplasm and Their Evaluation for Scab Management Strategies

Bacterial Endophyte Community Dynamics in Apple (Malus domestica Borkh.) Germplasm and Their Evaluation for Scab Management Strategies

In vitro assessment of Bacillus subtilis FJ3 affirms its biocontrol and plant growth promoting potential

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