Genomic insights into a sustained national outbreak of
            <i>Yersinia pseudotuberculosis</i>

Williamson, Deborah A.; Baines, Sarah L.; Carter, Glen P.; Silva, Anders Gonçalves da; Ren, Xiaoyun; Sherwood, Jill; Dufour, Muriel; Schultz, Mark B.; French, Nigel P.; Seemann, Torsten; Stinear, Timothy P.; Howden, Benjamin P

doi:10.1093/gbe/evw285

Cited by 23 publications

(30 citation statements)

References 33 publications

(39 reference statements)

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“…Исходя из представленных выше опубликованных результатов исследований, схему начальных этапов инфекционного процесса, вызываемого Y. pseudotuberculosis, можно представить следующим образом. Заражение человека и животных псевдотуберкулезом происходит преимущественно оральным путем с контаминированными возбудителем пищей или водой либо, значительно реже, в результате прямого контакта с инфицированными животными и людьми [70]. Поступившая из внешней среды, температура которой, как правило, составляет (10-30)°С, культура возбудителя имеет фенотип, резко отличающийся от такового культуры, выращенной при температуре ∼37°С -температуре тела теплокровных.…”

Section: адгезивность Y Pseudotuberculosis в патогенезе заболеванияunclassified

Yersinia pseudotuberculosis-derived adhesins

Byvalov

Konyshev

2019

Russian Journal of Infection and Immunity

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Around fifteen surface components referred to adhesins have been identified in Yersinia pseudotuberculosis combining primarily microbiological, molecular and genetic, as well as immunochemical and biophysical methods. Y. pseudotuberculosis-derived adhesins vary in structure and chemical composition but they are mainly presented by protein molecules. Some of them were shown to participate not only in adhesive but in other pathogen-related physiological functions in the host-parasite interplay. Adhesins can mediate bacterial adhesion to eukaryotic cell either directly or via the extracellular matrix components. These adhesion molecules are encoded by chromosomal DNA excepting YadA protein which gene is located in the calcium-dependence plasmid pYV common for pathogenic yersisniae. An optimum temperature for adhesin biosynthesis is located close to the body temperature of warm-blooded animals; however, at low temperature only invasin InvA, full-length smooth lipopolysaccharide and porin OmpF are produced in Y. pseudotuberculosis. Several adhesins (Psa, InvA) can be expressed at low pH (corresponds to intracellular content), thereby defining pathogenic yersiniae as facultative intracellular parasites. Three human Yersinia genus pathogens differ by ability to produce adhesins. Y. pseudotuberculosis adherence to host cells or extracellular matrix components is determined by a cumulative adhesion-based activity, which expression depends on chemical composition and physicochemical environmental conditions. It’s proposed that at the initial stage of infectious process adherence of Y. pseudotuberculosis to intestinal epithelium is mediated by InvA protein and “smooth” LPS form. These adhesins are produced in bacterial cells at low (lower than 30°С) temperature occurring in environment from which a pathogen invades into the host. At later stages of pathogenesis, after penetrating through intestinal epithelium, bacterial cells produce other adhesins, which promote survival and dissemination primarily into the mesenteric lymph nodes and, possibly, liver and spleen. At later stages of pathogenesis, after penetrating through intestinal epithelium, bacterial cells produce other adhesins, which promote survival and dissemination primarily into the mesenteric lymph nodes and, perhaps, liver and spleen. Qualitative and quantitative spectrum of Y. pseudotuberculosis adhesins is determined by environmental parameters (intercellular space, intracellular content within the diverse eukaryotic cells).

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Section: адгезивность Y Pseudotuberculosis в патогенезе заболеванияunclassified

Yersinia pseudotuberculosis-derived adhesins

Byvalov

Konyshev

2019

Russian Journal of Infection and Immunity

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“…Y. pseudotuberculosis is also found widely in the environment, including soil 6 , and in animals it causes tuberculosis-like disease 6 . Human cases of Y. pseudotuberculosis infections are usually sporadic, however several large outbreaks have been reported in Finland and recently in New Zealand 7,8 . Classical identification and typing of Y. pseudotuberculosis is based on the lipopolysaccharide Oantigen, resulting in a total of 21 known serotypes 9 .…”

Section: Three Species Ofmentioning

confidence: 99%

“…Classical identification and typing of Y. pseudotuberculosis is based on the lipopolysaccharide Oantigen, resulting in a total of 21 known serotypes 9 . However the efficacy of serotyping is very limited due to a large proportion of strains belonging to serotypes O:1a, O:1b, and O:3 8,10 .…”

Section: Three Species Ofmentioning

confidence: 99%

Phylogeographic separation and formation of sexually discrete lineages in a global population ofYersinia pseudotuberculosis

Seecharran

Karin-Mantarri

Koskela

et al. 2017

Preprint

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“…Classical identification and typing of Y. pseudotuberculosis is based on the lipopolysaccharide O-antigen, resulting in a total of 21 known serotypes [ 9 ]. However the efficacy of serotyping is very limited due to a large proportion of strains belonging to serotypes O : 1a, O : 1b and O : 3 [ 8, 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…To date the most comprehensive genome-scale analysis of Y. pseudotuberculosis centred around a country-wide outbreak in New Zealand [ 8 ]. Incorporation of publicly available genomes into this dataset also suggested a highly diverse species and that the New Zealand strains represented a geographically isolated clade of Y. pseudotuberculosis .…”

Section: Introductionmentioning

confidence: 99%

Phylogeographic separation and formation of sexually discrete lineages in a global population of Yersinia pseudotuberculosis

et al. 2017

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Yersinia pseudotuberculosis is a Gram-negative intestinal pathogen of humans and has been responsible for several nationwide gastrointestinal outbreaks. Large-scale population genomic studies have been performed on the other human pathogenic species of the genus Yersinia, Yersinia pestis and Yersinia enterocolitica allowing a high-resolution understanding of the ecology, evolution and dissemination of these pathogens. However, to date no purpose-designed large-scale global population genomic analysis of Y. pseudotuberculosis has been performed. Here we present analyses of the genomes of 134 strains of Y. pseudotuberculosis isolated from around the world, from multiple ecosystems since the 1960s. Our data display a phylogeographic split within the population, with an Asian ancestry and subsequent dispersal of successful clonal lineages into Europe and the rest of the world. These lineages can be differentiated by CRISPR cluster arrays, and we show that the lineages are limited with respect to inter-lineage genetic exchange. This restriction of genetic exchange maintains the discrete lineage structure in the population despite co-existence of lineages for thousands of years in multiple countries. Our data highlights how CRISPR can be informative of the evolutionary trajectory of bacterial lineages, and merits further study across bacteria.

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Genomic insights into a sustained national outbreak of Yersinia pseudotuberculosis

Cited by 23 publications

References 33 publications

Yersinia pseudotuberculosis-derived adhesins

Yersinia pseudotuberculosis-derived adhesins

Phylogeographic separation and formation of sexually discrete lineages in a global population ofYersinia pseudotuberculosis

Phylogeographic separation and formation of sexually discrete lineages in a global population of Yersinia pseudotuberculosis

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