Genomic, protein and antigenic variability of mycoplasma bovis

Poumarat, François; Solsona, Michel; Boldini, M.

doi:10.1016/0378-1135(94)90119-8

Cited by 41 publications

(33 citation statements)

References 18 publications

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“…Therefore, not surprisingly, the correlation between antigenic and genomic variability was limited to the detection of a general heterogeneity among isolates from different women and homogeneity among isolates from the same women in the present study. Lack of correlation between genomic and antigenic variability was previously reported by Poumarat et al [27] who examined genomic and antigenic variability of 37 M. bovis strains. The isolates were classified into 13 different genomic groups based on restriction endonuclease analysis with three enzymes.…”

Section: Discussionmentioning

confidence: 72%

Antigenic and genomic homogeneity of successive Mycoplasma hominis isolates

Jensen¹,

Thorsen²,

Møller³

et al. 1998

Journal of Medical Microbiology

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Sixty Mycoplasma horninis isolates were obtained from the cervices of pregnant women and from the ears or pharynges of their newborn babies. The isolates were examined by SDS-PAGE and pulsed-field gel electrophoresis. Antigenic and genomic profiles were obtained for 16 series with two or more successive isolates. Both analyses led to the conclusion that isolates from the same woman were identical or nearly identical, while isolates from different women exhibited a high degree of variation with respect to both genomic and antigenic profiles.

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Section: Discussionmentioning

confidence: 72%

Antigenic and genomic homogeneity of successive Mycoplasma hominis isolates

Jensen¹,

Thorsen²,

Møller³

et al. 1998

Journal of Medical Microbiology

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“…Mycoplasma proteins were fractioned by Triton X-114 phase partitioning and carried out according to the procedure of Bordier 5 adapted for mycoplasmas. 34,35 Solubilized proteins and standards were run on NuPage Novex 4%-12% Bis-Tris Zoom Gels e in the XCell SureLock Mini-Cell e according to vendor instructions. Gels were stained with SimplyBlue SafeStain e following standard procedures.…”

Section: Methodsmentioning

confidence: 99%

Molecular and Antigenic Characterization of aMycoplasma BovisStrain Causing an Outbreak of Infectious Keratoconjunctivitis

et al. 2006

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Abstract. An unusually high incidence of infectious keratoconjunctivitis followed by pneumonia and arthritis was observed in beef calves of a managed herd. No Moraxella spp. or bacteria other than Mycoplasma spp. were obtained from conjunctival and nasal swabs. A strategy was designed for characterization of bovine mycoplasmas at species and strain level on the basis of a combination of molecular tools and the immunoblotting method. The strategy made it possible to rapidly assign the bacterium responsible for this outbreak to one of the phylogenetic clusters of bovine mycoplasmas delineated in this study and then to identify it as Mycoplasma bovis. The strain, designated Sar 1, showed a 100% 16S rDNA sequence identity with two European strains (120/81 and MC3386) isolated in Germany and Ireland, respectively, and hosts a vsp gene analog to the vsp A , vsp , and vsp 422-8 genes of the M. bovis reference strain PG45 T and of the field strain 422. The use of a crossreactive rabbit serum developed against the Mycoplasma agalactiae immunodominant antigen P48 confirmed the molecular findings. The immunological response of calves against M. bovis was also investigated. This is the first report on the occurrence of M. bovis on the Island of Sardinia (Italy).

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“…Previously it has been shown that M. bovis contains an elaborate genetic system in which numerous genes encoding variable surface lipoproteins (Vsps) undergo spontaneous on-off switching to generate surface antigen variation (2,20). Sitespecific inversions within the vsp locus and intrachromosomal recombination between vsp genes enable Vsp diversification (12,13,14).…”

mentioning

confidence: 99%

Molecular Epidemiological Analysis of Mycoplasma bovis Isolates from the United Kingdom Shows Two Genetically Distinct Clusters

McAuliffe

Kokotovic²,

Ayling

et al. 2004

J Clin Microbiol

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Mycoplasma bovis is an important veterinary pathogen causing pneumonia, arthritis, and mastitis in infected cattle. We investigated the genetic diversity of 53 isolates collected in the United Kingdom between 1996 and 2002 with pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP), and random amplified polymorphic DNA (RAPD) analysis. In addition, the influence of variable surface protein (Vsp) profiles on the profiles generated with molecular typing techniques was studied. Both AFLP and RAPD separated the isolates into two distinct groups, but PFGE showed less congruence with the other techniques. There was no clear relationship between the geographic origin or year of isolation of the isolates and the profiles produced. No correlation between Vsp profiles and any of the molecular typing techniques was observed. We propose that RAPD and AFLP provide valuable tools for molecular typing of M. bovis.

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Genomic, protein and antigenic variability of mycoplasma bovis

Cited by 41 publications

References 18 publications

Antigenic and genomic homogeneity of successive Mycoplasma hominis isolates

Antigenic and genomic homogeneity of successive Mycoplasma hominis isolates

Molecular and Antigenic Characterization of aMycoplasma BovisStrain Causing an Outbreak of Infectious Keratoconjunctivitis

Molecular Epidemiological Analysis of Mycoplasma bovis Isolates from the United Kingdom Shows Two Genetically Distinct Clusters

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