Genomic regions responsible for amenability to Agrobacterium-mediated transformation in barley

Hisano, Hiroshi; Sato, Kazuhiro

doi:10.1038/srep37505

Cited by 36 publications

(16 citation statements)

References 35 publications

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“…The fragment was cloned into the Sac I sites of pBUH3-EGFP. The resulting plasmid vector was named pBUHWxTpGFP and used for Agrobacterium tumefaciens -mediated transformation in barley and rice, as described previously 25,26 .…”

Section: Methodsmentioning

confidence: 99%

Imaging Amyloplasts in the Developing Endosperm of Barley and Rice

Matsushima

Hisano

2019

Sci Rep

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Amyloplasts are plant-specific organelles responsible for starch biosynthesis and storage. Inside amyloplasts, starch forms insoluble particles, referred to as starch grains (SGs). SG morphology differs between species and SG morphology is particularly diverse in the endosperm of Poaceae plants, such as rice ( Oryza sativa ) and barley ( Hordeum vulgare ), which form compound SGs and simple SGs, respectively. SG morphology has been extensively imaged, but the comparative imaging of amyloplast morphology has been limited. In this study, SG-containing amyloplasts in the developing endosperm were visualized using stable transgenic barley and rice lines expressing amyloplast stroma-targeted green fluorescent protein fused to the transit peptide (TP) of granule-bound starch synthase I ( TP-GFP ). The TP-GFP barley and rice plants had elongated amyloplasts containing multiple SGs, with constrictions between the SGs. In barley, some amyloplasts were connected by narrow protrusions extending from their surfaces. Transgenic rice lines producing amyloplast membrane-localized SUBSTANDARD STARCH GRAIN6 (SSG6)-GFP were used to demonstrate that the developing amyloplasts contained multiple compound SGs. TP-GFP barley can be used to visualize the chloroplasts in leaves and other plastids in pollen and root in addition to the endosperm, therefore it provides as a useful tool to observe diverse plastids.

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Section: Methodsmentioning

confidence: 99%

Imaging Amyloplasts in the Developing Endosperm of Barley and Rice

Matsushima

Hisano

2019

Sci Rep

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“…Bernardo (4), for example, has pointed out the challenges of marker-assisted selection for complex traits, and application of CRISPR genome editing to barley is constrained by the trans-formability of barley genotypes. In this context, Golden Promise is one of the most transformation-amenable varieties known, and doubled haploids from the Oregon Promise population have been identified with Golden Promise levels of transformability (20).…”

Section: Discussionmentioning

confidence: 99%

Effects of Barley (Hordeum Vulgare L.) Variety and Growing Environment on Beer Flavor

Herb

Filichkin

Fisk

et al. 2017

Journal of the American Society of Brewing Chemists

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J. Am. Soc. Brew. Chem. 75(4): [345][346][347][348][349][350][351][352][353] 2017 This research tested the hypothesis that barley genotype can affect beer flavor and assessed the relative contributions of genotype and location to beer sensory descriptors. Golden Promise, Full Pint, 34 of their doubled haploid progeny, and CDC Copeland were grown at three locations in Oregon, U.S.A. Grain from these trials was micromalted and the resulting malts used for nano-brewing. Sensory evaluations were conducted on the nano-brews. Barley genotype had significant effects on many sensory descriptors. The most significant sensory descriptorswhen comparing barley genotypes-were cereal, color, floral, fruity, grassy, honey, malty, toasted, toffee, and sweet. Golden Promise was significantly higher in fruity, floral, and grassy flavors, whereas Full Pint was significantly higher in malty, toffee, and toasted flavors. CDC Copeland was closest to neutral for most flavor traits. There were notable differences for some descriptors between locations. New combinations of parental flavor attributes were observed in the progeny. Multitrait analysis revealed regions of the barley genome with significant effects on malting quality and flavor traits. These findings are, of course, applicable only to the barley germplasm tested, the environment sampled, and the protocols used for micromalting and brewing. The necessary largerscale experiments involving optimized malts and larger volumes of beer are in process.

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“…Indeed, the old Scottish malting cultivar Golden Promise is one of the few reliable haplotypes for Agrobacterium ( Agrobacterium tumefaciens )-mediated transformation. Hisano and Sato 56 identified loci controlling transformation amenability in the regions of chromosomes 2H and 3H in an F 2 population derived from a cross between the cultivars Golden Promise and Haruna Nijo. Introducing these genomic regions in target haplotypes may increase their transformation efficiency and genome-editing capabilities.…”

Section: Applied Use Of the Genomic Information Of Barleymentioning

confidence: 99%

History and future perspectives of barley genomics

Sato

2020

DNA Research

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Barley (Hordeum vulgare), one of the most widely cultivated cereal crops, possesses a large genome of 5.1 Gbp. Through various international collaborations, the genome has recently been sequenced and assembled at the chromosome level by exploiting available genetic and genomic resources. Many wild and cultivated barley accessions have been collected and preserved around the world. These accessions are crucial to obtain diverse natural and induced barley variants. The barley bioresource project aims to investigate the diversity of this crop based on purified seed and DNA samples of a large number of collected accessions. The long-term goal of this project is to analyze the genome sequences of major barley accessions worldwide. In view of technical limitations, a strategy has been employed to establish the exome structure of a selected number of accessions and to perform high-quality chromosome-scale assembly of the genomes of several major representative accessions. For the future project, an efficient annotation pipeline is essential for establishing the function of genomes and genes as well as for using this information for sequence-based digital barley breeding. In this article, I review the existing barley resources along with their applications and discuss possible future directions of research in barley genomics.

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Genomic regions responsible for amenability to Agrobacterium-mediated transformation in barley

Cited by 36 publications

References 35 publications

Imaging Amyloplasts in the Developing Endosperm of Barley and Rice

Imaging Amyloplasts in the Developing Endosperm of Barley and Rice

Effects of Barley (Hordeum Vulgare L.) Variety and Growing Environment on Beer Flavor

History and future perspectives of barley genomics

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