Genomic Sequence of the Yeast Kluyveromyces marxianus CCT 7735 (UFV-3), a Highly Lactose-Fermenting Yeast Isolated from the Brazilian Dairy Industry

Silveira, Wendel Batista da; Diniz, Raphael Hermano Santos; Cerdán, M. Esperanza; González-Siso, María Isabel; Souza, Robson de Assis; Vidigal, Pedro Marcus Pereira; Brustolini, Otávio J. B.; Prata, Emille R. B. de Almeida; Medeiros, Alexsandra Correia; Paiva, Lílian C.; Nascimento, Moysés; Ferreira, Éder G.; Santos, Valdilene Canazart dos; Bragança, Caio Roberto Soares; Fernandes, Tatiana Alves Rigamonte; Colombo, Lívia Tavares; Passos, Flávia Maria Lopes

doi:10.1128/genomea.01136-14

Cited by 20 publications

(7 citation statements)

References 6 publications

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“…Genomic and transcriptomic studies have started to shed light on K. marxianus and a growing number of genome sequences of K. marxianus strains are now available (Jeong et al, 2012 ; Silveira et al, 2014 ; Inokuma et al, 2015 ; Lertwattanasakul et al, 2015 ; Quarella et al, 2016 ). As yet, however, there has not been a systematic comparison of the sequenced K. marxianus genomes, nor a comparison to the single K. lactis genome that is in the public domain.…”

Section: Introductionmentioning

confidence: 99%

Ploidy Variation in Kluyveromyces marxianus Separates Dairy and Non-dairy Isolates

et al. 2018

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Kluyveromyces marxianus is traditionally associated with fermented dairy products, but can also be isolated from diverse non-dairy environments. Because of thermotolerance, rapid growth and other traits, many different strains are being developed for food and industrial applications but there is, as yet, little understanding of the genetic diversity or population genetics of this species. K. marxianus shows a high level of phenotypic variation but the only phenotype that has been clearly linked to a genetic polymorphism is lactose utilisation, which is controlled by variation in the LAC12 gene. The genomes of several strains have been sequenced in recent years and, in this study, we sequenced a further nine strains from different origins. Analysis of the Single Nucleotide Polymorphisms (SNPs) in 14 strains was carried out to examine genome structure and genetic diversity. SNP diversity in K. marxianus is relatively high, with up to 3% DNA sequence divergence between alleles. It was found that the isolates include haploid, diploid, and triploid strains, as shown by both SNP analysis and flow cytometry. Diploids and triploids contain long genomic tracts showing loss of heterozygosity (LOH). All six isolates from dairy environments were diploid or triploid, whereas 6 out 7 isolates from non-dairy environment were haploid. This also correlated with the presence of functional LAC12 alleles only in dairy haplotypes. The diploids were hybrids between a non-dairy and a dairy haplotype, whereas triploids included three copies of a dairy haplotype.

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Section: Introductionmentioning

confidence: 99%

Ploidy Variation in Kluyveromyces marxianus Separates Dairy and Non-dairy Isolates

et al. 2018

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“…Genomic and transcriptomic studies have started to shed light on K. marxianus, and a growing number of genome sequences of K. marxianus strains are now available. Those include KCTC 17555 [34], DMB1 [95], CCT 7735 [96], NBRC1777 [97], DMKU 3-1042 [35], B0399 [98], UFS-Y2791 [99], and other nine strains: L01, L02, L03, L04, L05, CBS397, NBRC0272, NBRC0288, and NBRC0617 [100].…”

Section: Complete Genome Sequence Of Thermotolerant Yeast K Marxianumentioning

confidence: 99%

Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries and Their Application in High- Temperature Fermentation

Kosaka¹,

Lertwattanasakul²,

NadchanokRodrussamee³

et al. 2019

Fuel Ethanol Production From Sugarcane

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Thermotolerant ethanologenic yeasts receive attention as alternative bio-ethanol producers to traditionally used yeast, Saccharomyces cerevisiae. Their utilization is expected to provide several benefits for bio-ethanol production due to their characteristics and robustness. They have been isolated from a wide variety of environments in a number of ASEAN countries: Thailand, Vietnam, Laos, and Indonesia. One of these yeasts, Kluyveromyces marxianus has been investigated regarding characteristics. Some strains efficiently utilize xylose, which is a main component of the 2nd generation biomass. In addition, the genetic basis of K. marxianus has been revealed by genomic sequencing and is exploited for further improvement of the strains by thermal adaptation or gene engineering techniques. Moreover, the glucose repression of K. marxianus and its mechanisms has been investigated. Results suggest that K. marxianus is an alternative to S. cerevisiae in next-generation bio-ethanol production industry. Indeed, we have succeeded to apply K. marxianus for bio-ethanol production in a newly developed process, which combines high-temperature fermentation with simultaneous fermentation and distillation under low pressure. This chapter aims to provide valuable information on thermotolerant ethanologenic yeasts and their application, which may direct the economic bioproduction of ethanol and other useful materials in the future.Recently, thermotolerant microorganisms were found among mesophiles with optimum growth temperatures that are 5-10°C higher than those of the typical mesophilic strains Fuel Ethanol Production from Sugarcane 122 Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries… http://dx.doi.org/10.5772/intechopen.79144 123Recently, there have been several reports on ethanol production at high temperatures using P. kudriavzevii (formerly known as I. orientalis). Several P. kudriavzevii strains were reported to grow and produce high levels of ethanol at high temperatures. The strain DMKU 3-ET15 was isolated from traditional fermented pork sausage in Thailand by an enrichment technique in a medium supplemented with 4% ethanol at 40°C. The strain produced 78.6 g/L ethanol from 180 g/L glucose at 40°C [20]. The strain KVMP10 that was isolated from soil located beneath apple trees for ethanol production from orange peel achieved 54 g/L ethanol at 42°C [48]. Strain RZ8-1 that was recently isolated from various samples collected from plant orchards in Thailand produced 33.8 g/L ethanol from 160 g/L glucose at 40°C [49].

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“…Sequencing of its genome was performed to gain more detailed information on its characteristics as a probiotic and to broaden knowledge on the genomic biodiversity in the species K. marxianus , since other strains whose genome sequences are available are used for bioethanol production or other applications ( 8 – 10 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of the Probiotic Yeast Kluyveromyces marxianus fragilis B0399

et al. 2016

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Genomic Sequence of the Yeast Kluyveromyces marxianus CCT 7735 (UFV-3), a Highly Lactose-Fermenting Yeast Isolated from the Brazilian Dairy Industry

Abstract: Here, we present the draft genome sequence of Kluyveromyces marxianus CCT 7735 (UFV-3), including the eight chromosomes and the mitochondrial genomic sequences.

Cited by 20 publications

References 6 publications

Ploidy Variation in Kluyveromyces marxianus Separates Dairy and Non-dairy Isolates

Ploidy Variation in Kluyveromyces marxianus Separates Dairy and Non-dairy Isolates

Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries and Their Application in High- Temperature Fermentation

Draft Genome Sequence of the Probiotic Yeast Kluyveromyces marxianus fragilis B0399

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