Genomic structure and characterization of mRNA expression pattern of porcine interferon gamma receptor 1 gene

Abstract

Interferon gamma receptor (IFNGR) plays an important role in the biological effects of IFN-γ. In this study, porcine IFNGR1 cDNA was cloned and two transcripts both having a coding region of 1413 bp were identified. Porcine IFNGR1 cDNA shares 62.95%, 63.73%, 72.90% and 81.10% identity in nucleotide sequence; and 45.64%, 46.69%, 58.04% and 72.55% homology in amino acid sequence to those of rat, mouse, human and cattle, respectively. The porcine IFNGR1 genomic structure consists of seven exons and six introns and is located on porcine chromosome 1. The mRNA expression of porcine IFNGR1 gene is detected in all tissues examined, with strong expression in spleen and liver tissues and weak expression in cerebrum, cerebellum and uterus tissues, respectively. A different developmental pattern in IFNGR1 mRNA expression between Laiwu and Duroc breeds was revealed by real-time quantitative RT-PCR: in Duroc pigs, a significantly higher expression was found in the tissues of heart (P<0.05), liver (P<0.01), kidney (P<0.01) and skeletal muscle (P<0.05) of adult pigs compared to piglets. In porcine reproductive and respiratory syndrome virus (PRRSV)-infected Dapulian pigs, compared to the uninfected ones, the expression level of IFNGR1 mRNA in spleen was significantly up-regulated (P<0.05), whereas its expression in the lymph node was significantly down-regulated (P<0.05); in PRRSV-infected Duroc × Yorkshire × Landrace commercial pigs, however, the differences both in spleen and lymph node tissues were not significant.

cDNA cloning, genomic structure and mRNA expression pattern of porcine type I interferons receptor 2 gene

“…The 5′ region of porcine IFNAR2 cDNA was amplified by using RACE‐PCR commercial GeneRacer™ kit (Invitrogen, CA, USA) as described in Xing et al. (2010).…”

“…Real‐time quantitative PCR (qPCR) was performed as is described in Xing et al. (2010) with primers 6F and 6R for IFNAR2 gene and pGAPDH‐F and pGAPDH‐R for GAPDH gene (Table 1).…”

“…Two animal experiments were performed as described by Xing et al (2010). Briefly, in the first experiment, both piglets and adult pigs of Laiwu and Duroc breeds were used for cDNA cloning and developmental dynamics analysis of IFNAR2 gene.…”

“…Real-time quantitative PCR (qPCR) was performed as is described in Xing et al (2010) with primers 6F and 6R for IFNAR2 gene and pGAPDH-F and pGAPDH-R for GAPDH gene (Table 1). The programme is 95°C for 10 min, followed by 40 cycles of 95°C for 30 s, 59°C (for IFNAR2) or 57°C (for GAPDH) for 30 s and 72°C for 30 s. The 2 )44CT method was used for calculation of relative gene expression (Liver & Schmittgen, 2001).…”

“Porcine Digestible Peptides (Pdp) in Weanling Diets Improves Gut Barrier Function, Immune Response and Nutrient Transport in Nursery Pigs”&nbsp;

“…TLR4 is a receptor involved in the recognition of lipopolysaccharide, a major cell wall component of Gram-negative bacteria [20], and IFNGR1 is part of the receptor that mediates the biological effects of IFN-γ [57]. The TLR4 and IFNGR1 genes have been reported to be upregulated in animals under stress and infection conditions in order to activate the innate immune response and ght against pathogens properly [21,29,58].…”