Genomics analysis of genes encoding respiratory burst oxidase homologs (RBOHs) in jatropha and the comparison with castor bean

Abstract

Respiratory burst oxidase homologs (RBOHs), which catalyze the production of superoxide from oxygen and NADPH, play key roles in plant growth and development, hormone signaling, and stress responses. Compared with extensive studies in model plants arabidopsis and rice, little is known about RBOHs in other species. This study presents a genome-wide analysis of Rboh family genes in jatropha (Jatropha curcas) as well as the comparison with castor bean (Ricinus communis), another economically important non-food oilseed crop of the Euphorbiaceae family. The family number of seven members identified from the jatropha genome is equal to that present in castor bean, and further phylogenetic analysis assigned these genes into seven groups named RBOHD, -C, -B, -E, -F, -N, and -H. In contrast to a high number of paralogs present in arabidopsis and rice that experienced several rounds of recent whole-genome duplications, no duplicate was identified in both jatropha and castor bean. Conserved synteny and one-to-one orthologous relationship were observed between jatropha and castor bean Rboh genes. Although exon-intron structures are usually highly conserved between orthologs, loss of certain introns was observed for JcRbohB, JcRbohD, and RcRbohN, supporting their divergence. Global gene expression profiling revealed diverse patterns of JcRbohs over various tissues. Moreover, expression patterns of JcRbohs during flower development as well as various stresses were also investigated. These findings will not only improve our knowledge on species-specific evolution of the Rboh gene family, but also provide valuable information for further functional analysis of Rboh genes in jatropha.

Genome Wide Identification of Respiratory Burst Oxidase Homolog (Rboh) Genes in Citrus sinensis and Functional Analysis of CsRbohD in Cold Tolerance

“…Prediction programs for subcellular localization showed that the seven CsRboh proteins were localized in the cell membrane. The results are in agreement with those obtained in other studies [ 10 , 13 , 14 , 16 , 28 ], suggesting Rbohs’ potential role in the regulation of ROS generation. Notably, few Rboh proteins were predicted to localize in chloroplast thylakoid membranes or mitochondria.…”

“…Here, we identified a total of seven Rboh genes from the Citrus sinensis genome ( Table 1 ). The number of Rboh genes in Citrus is similar to previous reports of grapes [ 8 ], strawberries [ 12 ], jatropha [ 13 ], cassava [ 14 ], and pepper [ 16 ], but relatively small. Prediction programs for subcellular localization showed that the seven CsRboh proteins were localized in the cell membrane.…”

“…Rbohs are encoded by multiple genes, and the gene family has been identified and characterized in some plant species. So far, ten Rboh genes were found in Arabidopsis [ 6 ], nine in rice [ 7 ], seven in grapes [ 8 ], fourteen in Nicotiana tabacum [ 9 ], twenty-six in upland cotton [ 10 ], nine in apples [ 11 ], seven in strawberries [ 12 ], seven in jatropha [ 13 ], eight in cassava [ 14 ], fourteen in Brassica rapa [ 15 ], and eight in pepper [ 16 ]. Analysis of gene expression patterns may help to understand the possible function of Rboh family genes.…”

Genome-wide identification and characterization of Respiratory Burst Oxidase Homolog genes in six Rosaceae species and an analysis of their effects on adventitious rooting in apple

“…RBOH enzymes catalyze ROS production and play multiple critical roles in plant development, biotic and abiotic stress responses, signal transduction and hormone responses [25,[46][47][48][49][50][51][52]. Hence, RBOH genes have been widely identified in diverse plant species [39][40][41][42][43][44]. In this study, 44 putative RBOH genes were identified from six horticultural Rosaceae species; their numbers ranged from a minimum of 4 to a maximum of 10 in the Rosaceae genomes (Table 1).…”

“…To investigate the evolutionary history of the RBOH family, we compared the number of RBOHs among 18 plant species [39][40][41][42][43][44]. As shown in Fig 1A, no individual species had more than 14 RBOH members.…”