Genomics-assisted breeding for ear rot resistances and reduced mycotoxin contamination in maize: methods, advances and prospects

Gaikpa, David Sewordor; Miedaner, Thomas

doi:10.1007/s00122-019-03412-2

Cited by 59 publications

(50 citation statements)

References 106 publications

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“…Moreover, in order to develop genetically resistant germplasm to Fusarium researchers carried out Quantitative Trait Locus (QTL) mapping analysis and GWAS by testing several distinct population structures (McMullen et al 2009;Yang et al 2010;Zila et al 2013Zila et al , 2014Warburton et al 2013;Chen et al 2016;Ju et al 2017;Maschietto et al 2017;Gaikpa and Miedaner 2019;Samayoa et al 2019;Septiani et al 2019).…”

Section: Introductionmentioning

confidence: 99%

A Genome-Wide Association Study To Understand the Effect of Fusarium verticillioides Infection on Seedlings of a Maize Diversity Panel

Stagnati

Rahjoo

Samayoa

et al. 2020

G3 Genes|Genomes|Genetics

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Fusarium verticillioides, which causes ear, kernel and stem rots, has been reported as the most prevalent species on maize worldwide. Kernel infection by F. verticillioides results in reduced seed yield and quality as well as fumonisin contamination, and may affect seedling traits like germination rate, entire plant seedling length and weight. Maize resistance to Fusarium is a quantitative and complex trait controlled by numerous genes with small effects. In the present work, a Genome Wide Association Study (GWAS) of traits related to Fusarium seedling rot was carried out in 230 lines of a maize association population using 226,446 SNP markers. Phenotypes were scored on artificially infected kernels applying the rolled towel assay screening method and three traits related to disease response were measured in inoculated and not-inoculated seedlings: plant seedling length (PL), plant seedling weight (PW) and germination rate (GERM). Overall, GWAS resulted in 42 SNPs significantly associated with the examined traits. Two and eleven SNPs were associated with PL in inoculated and not-inoculated samples, respectively. Additionally, six and one SNPs were associated with PW and GERM traits in not-inoculated kernels, and further nine and thirteen SNPs were associated to the same traits in inoculated kernels. Five genes containing the significant SNPs or physically closed to them were proposed for Fusarium resistance, and 18 out of 25 genes containing or adjacent to significant SNPs identified by GWAS in the current research co-localized within QTL regions previously reported for resistance to Fusarium seed rot, Fusarium ear rot and fumonisin accumulation. Furthermore, linkage disequilibrium analysis revealed an additional gene not directly observed by GWAS analysis. These findings could aid to better understand the complex interaction between maize and F. verticillioides.

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Section: Introductionmentioning

confidence: 99%

A Genome-Wide Association Study To Understand the Effect of Fusarium verticillioides Infection on Seedlings of a Maize Diversity Panel

Stagnati

Rahjoo

Samayoa

et al. 2020

G3 Genes|Genomes|Genetics

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“…The presence of mycotoxins, elements, and allergens in affected food crops has a high potential for mitigation via plant breeding Warburton and Williams, 2016;Arias et al, 2018;Gaikpa and Miedaner, 2019). These substances, produced by the fungus, the plant itself, or taken up by the plant from the environment, are generally not defense compounds, but can be severely detrimental to the health of humans and animals who consume the crop in which the substances have accumulated.…”

Section: Breeding Research To Understand the Opportunities To Reduce mentioning

confidence: 99%

Breeding Crops for Enhanced Food Safety

et al. 2020

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An increasing global population demands a continuous supply of nutritious and safe food. Edible products can be contaminated with biological (e.g., bacteria, virus, protozoa), chemical (e.g., heavy metals, mycotoxins), and physical hazards during production, storage, transport, processing, and/or meal preparation. The substantial impact of foodborne disease outbreaks on public health and the economy has led to multidisciplinary research aimed to understand the biology underlying the different contamination processes and how to mitigate food hazards. Here we review the knowledge, opportunities, and challenges of plant breeding as a tool to enhance the food safety of plant-based food products. First, we discuss the significant effect of plant genotypic and phenotypic variation in the contamination of plants by heavy metals, mycotoxin-producing fungi, and human pathogenic bacteria. In addition, we discuss the various factors (i.e., temperature, relative humidity, soil, microbiota, cultural practices, and plant developmental stage) that can influence the interaction between plant genetic diversity and contaminant. This exposes the necessity of a multidisciplinary approach to understand plant genotype × environment × microbe × management interactions. Moreover, we show that the numerous possibilities of crop/hazard combinations make the definition and identification of high-risk pairs, such as Salmonella-tomato and Escherichia coli-lettuce, imperative for breeding programs geared toward improving microbial safety of produce. Finally, we discuss research on developing effective assays and approaches for selecting desirable breeding germplasm. Overall, it is recognized that although breeding programs for some human pathogen/toxin systems are ongoing (e.g., Fusarium in wheat), it would be premature to start breeding when targets and testing systems are not well defined. Nevertheless, current research is paving the way toward this goal and this review highlights advances in the field and critical points for the success of this initiative that were discussed during the Breeding Crops for Enhanced Food Safety workshop held 5-6 June 2019 at University of California, Davis.

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“…Maize ear rot, including Gibberella ear rot (GER) and Fusarium ear rot (FER), caused by Fusarium graminearum (Schwabe) and Fusarium verticillioedes (Nirenberg), respectively, is a devastating disease in most maize-growing regions in the world [1]. Fusarium infections on maize not only reduce yields, but contaminate kernels with carcinogenic mycotoxins, particularly deoxynivalenol, zearalenone and fumonisins, which have potential threats to animal and human health [2,3,4,5].…”

Section: Introductionmentioning

confidence: 99%

“…Quantitative genetic variations for resistance to GER usually have moderate to high heritability among different maize germplasms [2], suggesting that phenotypic selection for improved GER resistance should be effective. However, resistance to GER in maize is easily influenced by environment conditions and genotype × environment interaction [2,7,8]. To improve the resistance of inbred lines and hybrids, multi-environmental inoculation trials are necessary for accurate phenotype evaluation in conventional maize breeding programs [9].…”

Section: Introductionmentioning

confidence: 99%

Identification of a major quantitative trait locus, qGER4.2, conferring resistance to Gibberella ear rot in maize using a Chinese recombination inbred line population

Zhou

et al. 2020

Preprint

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Background: Gibberella ear rot (GER), a prevalent disease caused by Fusarium graminearum, can result in yield loss and mycotoxin contamination in maize. Despite that several QTLs related to GER resistance have been reported previously, few of them was identified in Chinese maize inbred lines. In this study, we employed a Chinese recombination inbred line (RIL) population comprising of 204 lines, developed from a cross involving a resistant parent DH4866 and a susceptible line T877. The population was phenotypically evaluated at three field trial locations under artificial inoculation with F. graminearum and genotyped with an Affymetrix microarray CGMB56K SNP Array to detect the quantitative trait loci (QTLs) for resistance to GER. Results: Based on the genetic linkage map constructed using 1,868 bins as markers, a total of 15 QTLs were identified, and both DH4866 and T877 alleles at these QTLs contributed toward resistance. Of these QTLs, five were stably expressed across multiple locations, including three co-localized with previously reported genomic regions. The largest-effect QTL located on chromosome 4, qGER4.2, which accounted for 5.66~17.10% of the phenotypic variation, was detected at all locations. Based on the phenotypic values of the alleles corresponding to the five stable QTLs, a significant correlation (r2 = 0.45) between resistance to GER and the number of resistant alleles was observed. Conclusions: QTL mapping was successfully employed to identify genetic loci conferring resistance to GER by a high-density genetic map constructed from two Chinese maize germplasms. The five stable QTLs identified, especially the large-effect QTL, qGER4.2, will be useful in maize breeding programs aimed at improving GER resistance.

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Genomics-assisted breeding for ear rot resistances and reduced mycotoxin contamination in maize: methods, advances and prospects

Cited by 59 publications

References 106 publications

A Genome-Wide Association Study To Understand the Effect of Fusarium verticillioides Infection on Seedlings of a Maize Diversity Panel

A Genome-Wide Association Study To Understand the Effect of Fusarium verticillioides Infection on Seedlings of a Maize Diversity Panel

Breeding Crops for Enhanced Food Safety

Identification of a major quantitative trait locus, qGER4.2, conferring resistance to Gibberella ear rot in maize using a Chinese recombination inbred line population

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