Genomics for Wheat Improvement

Francki, Michael G.

doi:10.1007/978-90-481-2967-6_12

Cited by 5 publications

(1 citation statement)

References 163 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bread wheat possesses a collection of more than 1 million ESTs, derived from at least 42 cDNA libraries obtained from different stages of plant development and from imposition of different biotic and abiotic stress conditions (for recent review, see Francki, 2010). These sequences are available in the public domain through Genbank (http://ncbi.nlm.nih.gov/), and the collection provides an extensive resource for exploitation of the expressed genome portion for SNP discovery.…”

Section: Status Of Snp Discovery In Allopolyploid Cropsmentioning

confidence: 99%

Identification, characterization and interpretation of single‐nucleotide sequence variation in allopolyploid crop species

Kaur

Francki

Forster

2011

Plant Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

SummaryAn understanding of nature and extent of nucleotide sequence variation is required for programmes of discovery and characterization of single nucleotide polymorphisms (SNPs), which provide the most versatile class of molecular genetic marker. A majority of higher plant species are polyploids, and allopolyploidy, because of hybrid formation between closely related taxa, is very common. Mutational variation may arise both between allelic (homologous) sequences within individual subgenomes and between homoeologous sequences among subgenomes, in addition to paralogous variation between duplicated gene copies. Successful SNP validation in allopolyploids depends on differentiation of the sequence variation classes. A number of biological factors influence the feasibility of discrimination, including degree of gene family complexity, inbreeding or outbreeding reproductive habit, and the level of knowledge concerning progenitor diploid species. In addition, developments in high-throughput DNA sequencing and associated computational analysis provide general solutions for the genetic analysis of allopolyploids. These issues are explored in the context of experience from a range of allopolyploid species, representing grain (wheat and canola), forage (pasture legumes and grasses), and horticultural (strawberry) crop. Following SNP discovery, detection in routine genotyping applications also presents challenges for allopolyploids. Strategies based on either design of subgenome-specific SNP assays through homoeolocus-targeted polymerase chain reaction (PCR) amplification, or detection of incremental changes in nucleotide variant dosage, are described.

show abstract

Section: Status Of Snp Discovery In Allopolyploid Cropsmentioning

confidence: 99%

Identification, characterization and interpretation of single‐nucleotide sequence variation in allopolyploid crop species

Kaur

Francki

Forster

2011

Plant Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

show abstract

Bridging Genomic and Classical Breeding Approaches for Improving Crop Productivity

Rahman

Shaheen

Ashraf

et al. 2012

Crop Production for Agricultural Improvement

View full text Add to dashboard Cite

Chromosomal location of wheat genes of the carotenoid biosynthetic pathway and evidence for a catalase gene on chromosome 7A functionally associated with flour b* colour variation

Crawford

Francki

2013

Mol Genet Genomics

View full text Add to dashboard Cite

Knowledge of molecular and genetic mechanisms controlling wheat grain quality characteristics is significant for improving flour for end-product functionality. Flour b* colour is an important quality trait for breeding wheat varieties to produce grain for specific market requirements. The degree of flour yellowness is due to the accumulation of carotenoids in grain, particularly lutein. Flour b* is under polygenic control and quantitative trait loci (QTL) have frequently been reported on chromosome 7AL. Analysis of carotenoid genes showed that phytoene synthase (PSY) co-located to the QTL on 7AL but other genes at this locus are also thought to contribute flour b* colour variation. This study used the wheat genome survey sequence and identified the chromosomal location of all wheat carotenoid genes, but none other than PSY were located on 7AL and, therefore, other genes may control flour b* colour variation including oxidative genes that degrade carotenoids. An investigation of EST bin mapped to 7AL identified a gene encoding a catalase enzyme (Cat3-A1) that was phylogenetically related to other plant class III enzymes, co-located to the QTL for flour b* colour variation on 7AL in three mapping populations and expressed during seed development. Therefore, Cat3-A1 was functionally associated with flour b* colour variation. Catalase acts upon hydrogen peroxide as a substrate and it was postulated that Cat3-A1 alleles control varying degrees of bleaching action on lutein in developing wheat grain. Markers for Cat3-A1 developed in this study can be used in conjunction with other candidate gene markers including phytoene synthase and lycopene-ε-cylase to develop a molecular signature for selecting lines with specific flour b* colour values in wheat breeding.

show abstract

Genomics for Wheat Improvement

Cited by 5 publications

References 163 publications

Identification, characterization and interpretation of single‐nucleotide sequence variation in allopolyploid crop species

Identification, characterization and interpretation of single‐nucleotide sequence variation in allopolyploid crop species

Bridging Genomic and Classical Breeding Approaches for Improving Crop Productivity

Chromosomal location of wheat genes of the carotenoid biosynthetic pathway and evidence for a catalase gene on chromosome 7A functionally associated with flour b* colour variation

Contact Info

Product

Resources

About