Genotoxic evaluation of eugenol using the bone marrow micronucleus assay

Ellahueñe, Manuel F.; Pérez-Alzola, Luz Patricia; Orellana-Valdebenito, Margarita; Muñoz, Carlos; N, Lafuente-Indo

doi:10.1016/0165-1218(94)90044-2

Cited by 24 publications

(5 citation statements)

References 23 publications

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“…Hikiba et al (2005) reported a significant increase in the chromosomal aberrations in Syrian Hamster Embryo Cells treated with eugenol. In vivo study on mice indicated that eugenol is capable of increasing micronucleus frequency in polychromatic erythrocytes (Ellahuene et al 1994). By contrast, Chang et al (2000) reported that on human fibroblasts eugenol exhibited cytotoxic rather than genotoxic activity, and Maura et al (1989) failed to detect its effect at the chromosomal level using the bone marrow micronucleus test in rats.…”

Section: Discussionmentioning

confidence: 99%

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In vitro genotoxicity of root canal sealers

et al. 2009

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Section: Discussionmentioning

confidence: 99%

“…(2005) reported a significant increase in the chromosomal aberrations in Syrian Hamster Embryo Cells treated with eugenol. In vivo study on mice indicated that eugenol is capable of increasing micronucleus frequency in polychromatic erythrocytes (Ellahuene et al. 1994).…”

Section: Discussionmentioning

confidence: 99%

In vitro genotoxicity of root canal sealers

et al. 2009

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“…In the human pulp fibroblasts in vitro, eugenol did not show any genotoxic effect [38]. But using the bone marrow micronucleus assay in mice, eugenol showed a significant induction of micronucleus in 400 and 600 mg/kg doses [41]. Mutagenic capacity of eugenol was also demonstrated by in vivo eukaryotic assays on mice [42].…”

Section: Discussionmentioning

confidence: 82%

Genotoxicity of Some Essential Oils Frequently Used in Aromatherapy

Mezzoug¹,

Idaomar²,

Baudoux³

et al. 2016

ABB

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Genotoxic properties of the essential oils extracted from Artemisia dracunculus (tarragon), Ocimum basilicum (basil), Cinnamomum loureirii (cinnamon), Laurus nobilis (laurel), Satureja montana (savory) and Rosmarinus officinallis (rosemary) are studied by Drosophila melanogaster Somatic Mutation and Recombination Test (SMART). The high bioactivation crossed with a high cytochrome P450-dependent bioactivation capacity is used. This assay is principally based on the loss of heterozygosity of the suitable recessive markers' multiple wing hairs (mwh) and flare-3 (flr 3 ) which can lead to the formation of mutant clones of larval cells, and which are then going to be expressed as spots on the wings of adult flies. Third-instar larvae are treated for 48 hr with different concentrations of the essential oils dissolved in Tween-80 at 0.2% or 2%. The wings of the emerging adults are analyzed for the occurrence of different types of mutant spots. No statistically significant differences in spot frequencies between negative controls and treated series are observed. These results suggest that the six essential oils at concentrations tested are not genotoxic towards somatic cells of D. melanogaster.

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“…and Eug (0.7 g/kg, mice, p. o. ) ( Ichniowski and Hueper, 1946 ; Ellahueñe et al, 1994 ), indicating lower toxicity of AEE than Asp and Eug. In rats, after oral administration of AEE (50 mg/kg body weight) for 15 days, no obvious toxicity was detected ( Li et al, 2012b ).…”

Section: Introductionmentioning

confidence: 99%

Aspirin eugenol ester ameliorates LPS-induced inflammatory responses in RAW264.7 cells and mice

Liu,

Tao,

Shen

et al. 2023

Front. Pharmacol.

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Introduction: Inflammation is a defensive response of the body and the pathological basis of many diseases. However, excessive inflammation and chronic inflammation impair the homeostasis of the organism. Arachidonic acid (AA) has a close relationship with inflammation and is the main mediator of the pro-inflammatory response. Based on the prodrug principle, the new pharmaceutical compound aspirin eugenol ester (AEE) was designed and synthesized. However, the effects of AEE on key enzymes, metabolites and inflammatory signaling pathways in the AA metabolic network have not been reported.Methods: In this study, the anti-inflammation effects of AEE were first investigated in mice and RAW264.7 cells in LPS induced inflammation model. Then, the changes of the key enzymes and AA metabolites were explored by RT-PCR and targeted metabolomics. Moreover, the regulatory effects on NF–kB and MAPKS signaling pathways were explored by Western Blotting.Results: Results indicated that AEE significantly reduced the number of leukocyte and increased the lymphocyte percentage. AEE decreased the expression levels of IL-1β, IL-6, IL-8 and TNF-α both in vivo and in vitro. In the liver of mice, AEE downregulated the levels of AA, prostaglandin D2 (PGD2) and upregulated 12- hydroxyeicosatetraenoic acid (12-HETE). However, the changes of PGE2, PGF2α, 6-keto-prostaglandin F1α (6-KETO-PGF1α), 9-hydroxy-octadecenoic acid (9- HODE), 13-HODE, 15-HETE, docosahexaenoic acid (DHA) and thromboxane B2 (TXB2) were not significant. Additionally, it was found that AEE decreased the relative mRNA expression levels of p65 and p38 and the ratio of p-p65/p65.Discussion: It was concluded that AEE might inhibit the LPS-induced inflammatory response through the regulation of AA metabolism. This study provides the theoretical foundation for the development of AEE as a medicinal anti-inflammatory drug.

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Genotoxic evaluation of eugenol using the bone marrow micronucleus assay

Cited by 24 publications

References 23 publications

In vitro genotoxicity of root canal sealers

In vitro genotoxicity of root canal sealers

Genotoxicity of Some Essential Oils Frequently Used in Aromatherapy

Aspirin eugenol ester ameliorates LPS-induced inflammatory responses in RAW264.7 cells and mice

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