Genotoxicity Assessment and Protective Effect of Anogeissus leiocarpus Roots against Cyclophosphamide-Induced DNA Damage In Vivo

Motto, Aku Enam; Lawson-Evi, Povi; Diallo, Aboudoulatif; Eklu-Gadégbéku, Kwashie

doi:10.1155/2021/8020240

Cited by 8 publications

(6 citation statements)

References 32 publications

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“…Data in table [1] showed that, CP lead to a significant reduction in the RBCs count after 3 weeks. This reduction attributed to CP toxicity and antimitotic effects on bone marrow cells.…”

Section: Resultsmentioning

confidence: 94%

“…Table [1]: The protective effect of Zn Cl against Cyclophosphamide on peripheral blood cells at the end of study.…”

Section: Resultsmentioning

confidence: 99%

“…Cyclophosphamide [CP] has anticancer effects and harmful to normal cells, this bad effects may due to the imbalance of antioxidant system resulting in the accumulation of free radicals, Free radicals can cause DNA fragments, chromosomal aberrations [1] . Several studies demonstrated chromosomal aberrations caused by anticancer drugs in bone marrow cells [2,3] .…”

Section: Introductionmentioning

confidence: 99%

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Induced Chromosomal Aberrations of Cyclophosphamide in Bone Marrow Cells of Albino Rats and Possible Protective Role of Zinc Chloride

2022

International Journal of Medical Arts

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Article informationBackground: Chemotherapeutic agents are associated with different unwanted effects; the dangerous are mutagenic actions. Researchers do their best to ameliorate such effects by different supportive agents. Objective: The study designed to demonstrate the mutagenicity of the chemotherapeutic drugs especially cyclophosphamide [CP] and possible protective effects of Zinc Chloride [Zn Cl] Materials and Methods: The experiment was designed as 4 groups each group contain 12 animals, the first group [control group] was injected intraperitoneal normal saline [1 ml/kg] every other day for 20 days, the second group was given intraperitoneal Zn Cl [4 mg/kg] every other day for 20 days. The third group [Test group] injected by intraperitoneal cyclophosphamide [200 mg/kg] as a single dose on 15 th day of experiment, Fourth group [protective group] was given intraperitoneal ZnCl [4 mg/kg] every other day for 20 days and injected intraperitoneal single dose by CP [200 mg/kg] on 15 th day.Results: There were significant changes in blood cells, mitotic index and increased frequency of structural and numerical changes in chromosomes compared with the control group and these outcomes attributed to effects of free radicals of CP on health cells. The protective effect of Zn Cl which decrease blood cell changes, the structural and numerical aberrations in relation to the test group. Conclusion:CP has harmful effects on the blood cells and chromosomes. Zinc Chloride has a protective effect against the harmful effects of CP.

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“…Data in table [1] showed that, CP lead to a significant reduction in the RBCs count after 3 weeks. This reduction attributed to CP toxicity and antimitotic effects on bone marrow cells.…”

Section: Resultsmentioning

confidence: 94%

“…Table [1]: The protective effect of Zn Cl against Cyclophosphamide on peripheral blood cells at the end of study.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Induced Chromosomal Aberrations of Cyclophosphamide in Bone Marrow Cells of Albino Rats and Possible Protective Role of Zinc Chloride

2022

International Journal of Medical Arts

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“…When AMS35BB is associated with CYC , there is a tendency to potentiate the genotoxic effects of the chemotherapeutic drug. , This can be confirmed by the frequency of cell death in the liver and kidneys. Although the potentiation of micronuclei did not appear significant, the extent of damage increased significantly.…”

Section: Discussionmentioning

confidence: 90%

Chemotherapeutic Mechanism of Action of the Synthetic Resorcinolic Methyl 3,5-dimethoxy-2-octanoylbenzoate

Correa,

das Neves,

Oliveira

et al. 2024

Chem. Res. Toxicol.

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Resorcinolic lipids are described as potential examples of selective chemotherapeutic adjuvants that can enhance the effects of cyclophosphamide (CYC) while promoting cell death without causing DNA damage. Therefore, the current study attempted to describe how the resorcinolic lipid methyl 3,5-dimethoxy-2-octanoylbenzoate (AMS35BB) interacted with DNA (DNA docking) and how this compound affected genetic toxicology models and other biological characteristics when combined with CYC. We observed that AMS35BB, used alone (7.5 and 10 mg/kg), increases the frequency of genomic damage (comet assay) but not chromosomal damage (micronuclei assay), lowers phagocytosis, and promotes cell death in Swiss male mice. When used in association with CYC, AMS35BB can reduce the risk of genomic damage by up to 33.8% as well as chromosomal damage, splenic phagocytosis, cell death, and lymphocyte frequency. Molecular docking showed that AMS35BB had a higher affinity than the active metabolite of CYC for binding to the DNA double helix major groove. As a result, AMS35BB has the potential to be both an adjuvant when used in association with CYC and a therapeutic candidate for the development of a selective chemotherapeutic drug.

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“…The micronucleus test was conducted according to the OECD 474 guideline 20 . Mice were divided into five groups of five animals.…”

Section: Genotoxicity Evaluation By the Micronucleus Testmentioning

confidence: 99%

Assessment of in vivo and in ovo Toxicity of the Maytenus senegalensis Roots Hydroalcoholic Extract

Kpoyizoun¹,

Metowogo²,

Lawson-Evi³

et al. 2023

J. of Pharmacology and Toxicology

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Background and Objective: Maytenus senegalensis, is a plant traditionally used in Togo to treat several ailments including asthma. This study was undertaken to evaluate the toxicity of the rootʼs hydroalcoholic extract in vivo and in ovo. Materials and Methods: In vivo, the oral acute assay was conducted on Sprague Dawley rats by an oral administration of a single dose of 5000 mg kgG 1 . Subchronic toxicity was studied by a daily administration of the extract at 500 and 1000 mg kgG 1 for 28 consecutive days. The micronucleus test was performed on ICR mice treated with the extract at 500, 1000 and 2000 mg kgG 1 for 7 consecutive days to assess its genotoxicity. In ovo, the effect of the extract injection to SASSO hen eggs on chick embryo development was studied. Results: The acute toxicity test showed that the extract LD 50 was over 5000 mg kgG 1 . The subchronic toxicity study showed no significant variation in the ratʼs body weight increment and haematological and biochemical parameters. Similarly, organsʼ relative weight and histology were not affected by the Maytenus senegalensis extract administration. The micronucleus test revealed an increase (p<0.05) in the number of Micronuclei in Polychromatic Erythrocytes (MnPCE) in mice receiving the extract at 2000 mg kgG 1 and a decrease (p<0.05) in the percentage of polychromatic erythrocyte in the 1000 and 2000 mg kgG 1 groups. Administrated in ovo, the extract did not damage blood vessels or significantly affect the relative weights of the albumen and embryo. Conclusion: Maytenus senegalensis hydroalcoholic extract can be considered safe regarding the results of acute and subacute toxicity tests but further investigations are needed to state its genotoxicity and embryonic toxicity.

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Genotoxicity Assessment and Protective Effect of Anogeissus leiocarpus Roots against Cyclophosphamide-Induced DNA Damage In Vivo

Cited by 8 publications

References 32 publications

Induced Chromosomal Aberrations of Cyclophosphamide in Bone Marrow Cells of Albino Rats and Possible Protective Role of Zinc Chloride

Induced Chromosomal Aberrations of Cyclophosphamide in Bone Marrow Cells of Albino Rats and Possible Protective Role of Zinc Chloride

Chemotherapeutic Mechanism of Action of the Synthetic Resorcinolic Methyl 3,5-dimethoxy-2-octanoylbenzoate

Assessment of in vivo and in ovo Toxicity of the Maytenus senegalensis Roots Hydroalcoholic Extract

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