Genotoxicity Evaluation of Titanium Dioxide Nanoparticles In Vitro: a Systematic Review of the Literature and Meta-analysis

Ling, Chunmei; An, Hongmei; Li, Li; Wang, Jiaqi; Lu, Tianjiao; Wang, Haixia; Hu, Yunhua; Song, Guanling; Liu, Sixiu

doi:10.1007/s12011-020-02311-8

Cited by 29 publications

(30 citation statements)

References 80 publications

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“…Numerous papers addressing the genotoxicity of NMs investigated by one of the variants of the in vitro micronucleus assay have been published [ 37 , 38 ]. The majority of them have focused on the various size fractions of titanium dioxide (a possible carcinogen to humans according to the International Agency for Research on Cancer [ 39 ], group 2B), one of the most common NMs used in industry.…”

Section: Discussionmentioning

confidence: 99%

Testing Strategies of the In Vitro Micronucleus Assay for the Genotoxicity Assessment of Nanomaterials in BEAS-2B Cells

et al. 2021

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The evaluation of the frequency of micronuclei (MN) is a broadly utilised approach in in vitro toxicity testing. Nevertheless, the specific properties of nanomaterials (NMs) give rise to concerns regarding the optimal methodological variants of the MN assay. In bronchial epithelial cells (BEAS-2B), we tested the genotoxicity of five types of NMs (TiO2: NM101, NM103; SiO2: NM200; Ag: NM300K, NM302) using four variants of MN protocols, differing in the time of exposure and the application of cytochalasin-B combined with the simultaneous and delayed co-treatment with NMs. Using transmission electron microscopy, we evaluated the impact of cytochalasin-B on the transport of NMs into the cells. To assess the behaviour of NMs in a culture media for individual testing conditions, we used dynamic light scattering measurement. The presence of NMs in the cells, their intracellular aggregation and dispersion properties were comparable when tests with or without cytochalasin-B were performed. The genotoxic potential of various TiO2 and Ag particles differed (NM101 < NM103 and NM302 < NM300K, respectively). The application of cytochalasin-B tended to increase the percentage of aberrant cells. In conclusion, the comparison of the testing strategies revealed that the level of DNA damage induced by NMs is affected by the selected methodological approach. This fact should be considered in the interpretation of the results of genotoxicity tests.

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Section: Discussionmentioning

confidence: 99%

Testing Strategies of the In Vitro Micronucleus Assay for the Genotoxicity Assessment of Nanomaterials in BEAS-2B Cells

et al. 2021

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“…Several studies show that genotoxicity of NMs not only depends on dose and exposure duration, but also on their size, surface properties, chemical composition as well as on shape [48][49][50][51].…”

Section: General Mechanisms Of Genotoxicitymentioning

confidence: 99%

“…Initially this biomarker was used for identifying ionizing radiation effects [133]. In the last years γH2AX phosphorylation technique has been used in several studies to measure DSB caused by different NMs [15] for instance carbon nanotubes [134,135], zinc oxide [136], gold [137], silica [138], polystyrene [139] and titanium dioxide NMs [50] showing that this technique can be a useful tool to assess the genotoxic potential of NMs [15].…”

Section: Dna Damagementioning

confidence: 99%

“…Different approaches have been proposed until now to increase the speed of the micronucleus assay in vitro. Classically, the long and tedious visual scoring of slides has been changed by using automated platforms scoring different set of slides in each run [50,60,[72][73][74]. The micronucleus assay using HTS/HCS platforms has been widely used in NM research; it requires less test material than conventional test methods, and has a greater compatibility with high throughput screening instrumentation [75][76][77][78][180][181][182].…”

Section: In Vitro Micronucleus Assaymentioning

confidence: 99%

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Genotoxicity of Nanomaterials: Advanced In Vitro Models and High Throughput Methods for Human Hazard Assessment—A Review

et al. 2020

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Changes in the genetic material can lead to serious human health defects, as mutations in somatic cells may cause cancer and can contribute to other chronic diseases. Genotoxic events can appear at both the DNA, chromosomal or (during mitosis) whole genome level. The study of mechanisms leading to genotoxicity is crucially important, as well as the detection of potentially genotoxic compounds. We consider the current state of the art and describe here the main endpoints applied in standard human in vitro models as well as new advanced 3D models that are closer to the in vivo situation. We performed a literature review of in vitro studies published from 2000–2020 (August) dedicated to the genotoxicity of nanomaterials (NMs) in new models. Methods suitable for detection of genotoxicity of NMs will be presented with a focus on advances in miniaturization, organ-on-a-chip and high throughput methods.

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“…Due to the widespread use of TiO 2 , exposure to NPs by ingestion, inhalation, or sorption has become virtually inevitable. In the last five years, reviews have been published on the effects of TiO 2 NPs in living organisms, focusing either on the toxicology of TiO 2 NPs [ 1 , 2 , 11 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ], safety concerns in various applications [ 24 , 25 , 26 , 27 , 28 ] and related impact on human health [ 28 , 29 , 30 , 31 , 32 , 33 ], or their effects on water/soil/environmental quality [ 34 , 35 , 36 , 37 , 38 ]. Such review studies are necessary to conclusively determine the environmental and human effects of TiO 2 NPs.…”

Section: Introductionmentioning

confidence: 99%

Influence of Titanium Dioxide Nanoparticles on Human Health and the Environment

2021

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Nanotechnology has enabled tremendous breakthroughs in the development of materials and, nowadays, is well established in various economic fields. Among the various nanomaterials, TiO2 nanoparticles (NPs) occupy a special position, as they are distinguished by their high availability, high photocatalytic activity, and favorable price, which make them useful in the production of paints, plastics, paper, cosmetics, food, furniture, etc. In textiles, TiO2 NPs are widely used in chemical finishing processes to impart various protective functional properties to the fibers for the production of high-tech textile products with high added value. Such applications contribute to the overall consumption of TiO2 NPs, which gives rise to reasonable considerations about the impact of TiO2 NPs on human health and the environment, and debates regarding whether the extent of the benefits gained from the use of TiO2 NPs justifies the potential risks. In this study, different TiO2 NP exposure modes are discussed, and their toxicity mechanisms—evaluated in various in vitro and in vivo studies—are briefly described, considering the molecular interactions with human health and the environment. In addition, in the conclusion of this study, the toxicity and biocompatibility of TiO2 NPs are discussed, along with relevant risk management strategies.

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Genotoxicity Evaluation of Titanium Dioxide Nanoparticles In Vitro: a Systematic Review of the Literature and Meta-analysis

Cited by 29 publications

References 80 publications

Testing Strategies of the In Vitro Micronucleus Assay for the Genotoxicity Assessment of Nanomaterials in BEAS-2B Cells

Testing Strategies of the In Vitro Micronucleus Assay for the Genotoxicity Assessment of Nanomaterials in BEAS-2B Cells

Genotoxicity of Nanomaterials: Advanced In Vitro Models and High Throughput Methods for Human Hazard Assessment—A Review

Influence of Titanium Dioxide Nanoparticles on Human Health and the Environment

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