Genotoxicity in the rivers from the Brantas catchment (East Java, Indonesia): occurrence in sediments and effects in Oreochromis niloticus (Linnæus 1758)

Risjani, Yenny; Couteau, Jérôme; Yunianta, Yunianta; Widowati, Ita; Hermawati, A.; Minier, Christophe

doi:10.1007/s11356-020-08575-w

Cited by 20 publications

(7 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MN assay is a potent tool for the genotoxicity evaluation given to its simplicity, reliability and high sensitivity to xenobiotic compounds. The MN test using fish erythrocytes have been reported in many species for the assessment of aquatic pollutants (Obiakor et al, 2021; Risjani et al, 2020; Verma et al, 2021). Significant induction of MN after exposure to commercial grade CYP detected in the present investigation are in agreement with earlier studies of pesticide exposure in other fish species (Bano et al, 2021; D'Costa et al, 2018; Ismail et al, 2018; Oladokun et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

In vivo genotoxic effects of commercial grade cypermethrin on fish peripheral erythrocytes

Sharma

Jindal

2022

Environ and Mol Mutagen

View full text Add to dashboard Cite

The study explicates the genotoxic effects of commercial grade cypermethrin on peripheral erythrocytes of Catla catla, chronically exposed to two environmentally relevant concentrations. The fish was treated with sub-lethal concentrations 0.12 μg/L and 0.41 μg/L (1/10th and /1/3rd of 96 h LC 50 ) of cypermethrin for 45 days. DNA damage in the exposed fish was assessed using alkaline comet assay, presence of micronuclei (MN), erythrocyte nuclear and cytoplasmic abnormalities.Exposure to cypermethrin induced a dose-dependent increase in percent DNA damage, micronucleus frequency and erythrocyte abnormalities. Nuclear anomalies such as notched nuclei, lobed nuclei, bridged nuclei, and deformed nuclei; and cytoplasmic anomalies like anisochromasia, vacuolated cytoplasm, lobed cells, and echinocytes were observed. The findings revealed the genotoxic potential of commercial formulations pyrethroid cypermethrin at concentrations found in the environment and their potential deleterious effects on nontarget aquatic organisms.

show abstract

Section: Discussionmentioning

confidence: 99%

In vivo genotoxic effects of commercial grade cypermethrin on fish peripheral erythrocytes

Sharma

Jindal

2022

Environ and Mol Mutagen

View full text Add to dashboard Cite

show abstract

“…There exist various methods, approaches and animal models to analyze toxicity via biochemical, cellular or mortality assays [10]. Indeed, the toxicity test using a rat model shows that the Porphyridium biomass was not toxic [36].…”

Section: Discussionmentioning

confidence: 99%

“…An interesting phenomenon was noticed on the ninth day post-infectious treatment with Vibrio harveyi, where the respiratory burst (RB) value was lower than that on the first, seventh and eighth days (0.511, 0.395, 0.537, and 0.383, respectively) (Figure 10). There exist various methods, approaches and animal models to analyze toxicity via biochemical, cellular or mortality assays [10]. Indeed, the toxicity test using a rat model shows that the Porphyridium biomass was not toxic [36].…”

Section: Respiratory Burst (Rb) Activitymentioning

confidence: 99%

Exopolysaccharide from Porphyridium cruentum (purpureum) is Not Toxic and Stimulates Immune Response against Vibriosis: The Assessment Using Zebrafish and White Shrimp Litopenaeus vannamei

et al. 2021

Self Cite

View full text Add to dashboard Cite

Exopolysaccharides, or extracellular polysaccharides (EPS, sPS), represent a valuable metabolite compound synthesized from red microalgae. It is a non-toxic natural agent and can be applied as an immunostimulant. The toxicity test of exopolysaccharides from Porphyridium has been done in vivo using zebrafish (Danio rerio) embryonic model, or the ZET (zebrafish embryotoxicity test). The administration of extracellular polysaccharides or exopolysaccharides (EPS) from microalgae Porphyridium cruentum (synonym: P. purpureum) to shrimps Litopenaeus vannamei was investigated to determine the effect of this immunostimulant on their non-specific immune response and to test if this compound can be used as a protective agent for shrimps in relation to Vibrio infection. For immune response, exopolysaccharides were given to shrimps via the immersion method on day 1 and booster on day 8. Shrimp hemocytes were taken on day 1 (EPS administration), day 7 (no treatment), day 8 (EPS booster) and day 9 (Vibrio infection) and tested for their immune response on each treatment. The result shows that the EPS is not toxic, as represented by the normal embryonic development and the mortality data. In the Pacific white shrimps, an increase in the values of all immune parameters was shown, in line with the increasing EPS concentration, except for the differential hemocyte count (DHC). In detail, an increase was noted in total hemocytes (THC) value, phagocytotic activity (PA) and respiratory burst (RB) in line with the EPS concentration increase. These results and other previous studies indicate that EPS from Porphyridium is safe, enhances immune parameters in shrimp rapidly, and has the ability to act as an immunostimulant or an immunomodulator. It is a good modulator for the non-specific immune cells of Pacific white shrimps, and it can be used as a preventive agent against vibriosis.

show abstract

“…A question can be arising regarding the toxicity of the compound for the testing animals. It exist various methods, approaches and animal models to analyze toxicity, from biochemical, cellular or mortality assays [39]. Indeed, the toxicity test using rat model shows that Phorphyridium biomass was not toxic [41].…”

Section: Discussionmentioning

confidence: 99%

Extracellular Polysaccharide from <em>Porphyridium cruentum (purpureum) S</em>timulates the Non-Specific Immune Activities on White Shrimp (<em>Litopenaeus vannamei</em>)

Risjani¹,

Mutmainnah²,

Wulan³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

White shrimps are susceptible to outbreaks of vibriosis because they do not have any adaptive immune system, they only have a non-specific innate immune system. The administration of EPS from microalgae Porphyridium cruentum (synonym: P. purpureum) on shrimps Litopenaeus vannamei was investigated to determine the effect of this immunostimulant on their non specific immune response and to test if EPS can be used as a protective agent for shrimp related to Vibrio infection. EPS was given to shrimps by immersion method on day 1 and booster on day 8. Shrimp hemocytes were taken on day 1 (EPS administration), day 7 (no treatment), day 8 (EPS booster) and day 9 (Vibrio infection) and tested for their immune response on each treatment. Result shows an increase in values of all immune parameters in line with the increasing EPS concentration, except the Differential Haemocyte Count (DHC). In detail, an increase was noted in total hemocytes (THC) value, Phagocytotic Activity (PA), Respiratory Burst (RB) in line as the EPS concentration increase. Although there is a decrease after the infection, the value obtained is not lower than the control value. These results indicate that EPS from Porphyrydium enhances immune parameters in shrimp rapidly and has the ability as an immunostimulant or an immunomodulator. It is a good modulator for the non specific immune cells of Pacific white shrimps, and it can be used as a preventive agent against Vibrio.

show abstract

Genotoxicity in the rivers from the Brantas catchment (East Java, Indonesia): occurrence in sediments and effects in Oreochromis niloticus (Linnæus 1758)

Cited by 20 publications

References 35 publications

In vivo genotoxic effects of commercial grade cypermethrin on fish peripheral erythrocytes

In vivo genotoxic effects of commercial grade cypermethrin on fish peripheral erythrocytes

Exopolysaccharide from Porphyridium cruentum (purpureum) is Not Toxic and Stimulates Immune Response against Vibriosis: The Assessment Using Zebrafish and White Shrimp Litopenaeus vannamei

Extracellular Polysaccharide from <em>Porphyridium cruentum (purpureum) S</em>timulates the Non-Specific Immune Activities on White Shrimp (<em>Litopenaeus vannamei</em>)

Contact Info

Product

Resources

About