“…Different breeding strategies including diversity analysis, introduction and stability analysis of exotic germplasm, hybridization, heterosis breeding, mutagenesis and markerassisted selection are practiced for exploring maize germplasm and development of pro-vitamin A enriched cultivars (Maqbool et al, 2018). Genome-wide association variety and development of transgenic maize genotypes are also being practiced, whereas RNA interference and genome editing tools could also be used as potential strategies for provitamin A biofortification of maize genotypes (Shrestha et al, 2016). The use of these breeding strategies for provitamin A biofortification of maize is comprehensively reviewed to provide a working outline for maize breeders (Maqbool et al, 2018) The biofortified maize varieties were better sources of most of the essential amino acids comparative to the white variety, but, similar to the white maize, they were deficient in histidine and lysine (Kirthee et al, 2013).…”