2018
DOI: 10.1038/s41598-018-25596-1
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Genotype I of Japanese Encephalitis Virus Virus-like Particles Elicit Sterilizing Immunity against Genotype I and III Viral Challenge in Swine

Abstract: Swine are a critical amplifying host involved in human Japanese encephalitis (JE) outbreaks. Cross-genotypic immunogenicity and sterile protection are important for the current genotype III (GIII) virus-derived vaccines in swine, especially now that emerging genotype I (GI) JE virus (JEV) has replaced GIII virus as the dominant strain. Herein, we aimed to develop a system to generate GI JEV virus-like particles (VLPs) and evaluate the immunogenicity and protection of the GI vaccine candidate in mice and specif… Show more

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Cited by 20 publications
(24 citation statements)
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“…These results were consistent with a previous observation that GI virus-like particles failed to cross-protect 10% of vaccinated mice against GIII viral challenge [29]. However, swine immunized with these GI virus-like particles showed no fever, viremia or viral RNA in tissues after GI or GIII viral challenge, demonstrating sterile protection against GI and GIII viral infection in swine [29]. Therefore, more detailed studies are needed to evaluate the cross-protective efficacy of GI-derived vaccines against GIII viral infection.…”
Section: Discussionsupporting
confidence: 93%
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“…These results were consistent with a previous observation that GI virus-like particles failed to cross-protect 10% of vaccinated mice against GIII viral challenge [29]. However, swine immunized with these GI virus-like particles showed no fever, viremia or viral RNA in tissues after GI or GIII viral challenge, demonstrating sterile protection against GI and GIII viral infection in swine [29]. Therefore, more detailed studies are needed to evaluate the cross-protective efficacy of GI-derived vaccines against GIII viral infection.…”
Section: Discussionsupporting
confidence: 93%
“…Given the emergence of the GI virus as the dominant genotype in Asian regions and the partial cross-protection efficacy of GIII-derived vaccines against GI viral infection, development of a GI-derived vaccine has been proposed to control GI viral infection [25,28,29]. We therefore examined the protective efficacy of a GI-derived vaccine against the challenge of GI and GIII viruses.…”
Section: Resultsmentioning
confidence: 99%
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“…Immunization of mice with the mutant VLP elicited similar immunogenicity and protection to those with the native cleavage site. Similar immunogenicity/protection in mice and pigs was respectively observed when plasmids encoding JEV prME genes were stably expressed in rabbit kidney-derived RK13 and CHO cells [40,41]. Following the above discoveries, several other groups also expressed plasmid vectors encoding JEV prME genes to produce JEV VLPs in different mammalian cells, yielding similar immunogenicity/protection in mice [42,43].…”
Section: Japanese Encephalitis Virusmentioning
confidence: 74%
“…However, the expression of toxic E glycoproteins could limit the generation of stable cell lines capable of producing higher yields of VLPs in mammalian cells [24][25][26]. Nonetheless, JEV GI VLPs produced from stable mammalian cell lines have been shown to induce immunity against GI and GIII JEV in mice and swine [27]. The conventional baculovirus expression system has been employed to produce large quantities of JEV VLPs in insect cells [28,29]; however, the antigenicity of Lepidoptera-derived JEV VLPs has yet to be elucidated.…”
Section: Introductionmentioning
confidence: 99%