Genotypes of Japanese Encephalitis Virus Isolated in Three States in Malaysia

Tsuchie, Hideaki; Oda, Kazumasa; Vythilingam, Indra; Thayan, Ravindran; Vijayamalar, B; Sinniah, M; Singh, Jasbir; Wada, T.; Tazaki, Hiroshi; Kurimura, Takashi; Igarashi, Akira

doi:10.4269/ajtmh.1997.56.153

Cited by 29 publications

(24 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[17][18][19][20] Tembusu virus is one of such viruses that was isolated from mosquitoes in Kuala Lumpur in 1955. 17,21 Since then, Tembusu virus has been isolated from mosquitoes of Culex species in Peninsular Malaysia, East Malaysia (Sarawak), and Thailand.…”

Section: Discussionmentioning

confidence: 99%

Encephalitis and retarded growth of chicks caused by Sitiawan virus, a new isolate belonging to the genus Flavivirus.

Kono¹,

Tsukamoto²,

Hamid³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

100

View full text Add to dashboard Cite

Abstract.A new virus named Sitiawan virus (SV) was isolated from sick broiler chicks in chicken embryos. The virus replicated well with cytopathogenic effect (CPE) in the chicken B-lymphocyte cell line LSCC-BK3. The virus was an enveloped RNA virus of approximately 41 nm in size with hemagglutinating activity (HA) to goose erythrocytes. It was cross-reactive with Japanese encephalitis virus (JEV), a member of flaviviruses by HA inhibition tests but not by cross-virus neutralization tests. The cDNA fragment of NS5 gene was amplified with primers corresponding to NS5 gene of flaviviruses. The nucleotide sequences were 92% homologous to Tembusu virus, a member of the mosquito-borne virus cluster of the genus Flavivirus. In cross-neutralization tests with Tembusu virus, antiserum to SV did not neutralize Tembusu virus, and antiserum to Tembusu virus neutralized more weakly to SV than against homologous virus. These results indicate that SV is a new virus which can be differentiated serologically from Tembusu virus but is otherwise similar with respect to nucleotide sequence. The virus causes encephalitis, growth retardation, and increased blood glucose levels in inoculated chicks.

show abstract

Section: Discussionmentioning

confidence: 99%

Encephalitis and retarded growth of chicks caused by Sitiawan virus, a new isolate belonging to the genus Flavivirus.

Kono¹,

Tsukamoto²,

Hamid³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

100

View full text Add to dashboard Cite

show abstract

“…Genotype I is found primarily in Laos, northern Thailand, Cambodia, and Malaysia; Genotype II consists of isolates from southern Thailand, Malaysia, and Indonesia; Genotype III appears to be the most widely distributed and includes isolates from Japan, Korea, China, Malaysia, Philippines, Indonesia, India, and Sri Lanka; and Genotype IV appears to be restricted to Indonesia. [8][9][10] Comparison of the genetic sequence of the 1995 isolates of JE virus in the prM region revealed they probably had a common source and were most closely related to a 1970 isolate from Malaysia and a 1981 isolate from Indonesia, both of which belonged to Genotype II. 5 Sequence comparison of an overlapping region of the non-structural and 3Ј untranslated region (NS5-3Ј UTR) confirmed the relationship between the Australian, Indonesian, and Malaysian isolates, and also revealed the Australian isolates had a unique eleven base deletion immediately following the stop codon in the 3Ј untranslated region.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Japanese encephalitis virus from mosquitoes (Diptera: Culicidae) collected in the Western Province of Papua New Guinea, 1997-1998.

Johansen¹,

Hurk²,

Ritchie³

et al. 2000

Am J Trop Med Hyg

104

109

View full text Add to dashboard Cite

After Japanese encephalitis (JE) virus emerged in the Torres Strait in Australia in 1995, investigations were initiated into the origin of the incursion. New Guinea was considered the most likely source, given its proximity to islands of the Torres Strait. Almost 400,000 adult mosquitoes were processed for virus isolation from 26 locations in the Western Province of Papua New Guinea (PNG) between February 1996 and February 1998, yielding three isolates of JE virus. Two isolates of Murray Valley encephalitis, 17 isolates of Sindbis, and 1 each of Sepik and Ross River viruses were also obtained. Nucleic acid sequences of the PNG JE isolates were determined in the prM region, and in a region overlapping a part of the fifth nonstructural protein and the 3' untranslated region. The PNG isolates belonged to genotype II, and shared > 99.2% identity with isolates from humans and mosquitoes from the Torres Strait, suggesting that PNG is the source of incursions of JE virus into Australia.

show abstract

“…Nucleotide sequences in the C/preM region of cluster 1, cluster 2, and cluster 3 isolates were compared with those of isolates from Asia that have been studied previously. [13][14][15][16][17][18] Phylogenetic analysis showed that cluster 1 is more specific to Taiwan than cluster 2 and cluster 3. The Japanese virus, JaOArS982, isolated in 1982, was closer to isolates of cluster 1 (homology 96.5-98.6%) than those of the Osaka-79 (homology 95-97.1%), and India 6309-82 (homology 95-96.7%) strains (dendrogram not shown).…”

Section: Resultsmentioning

confidence: 99%

“…Strain variation among JE virus isolates has been demonstrated by serological tests, [29][30][31] nucleotide fingerprinting, 32,33 and nucleotide sequencing techniques. [13][14][15][16][17][18] Primer-extension sequencing of genomic RNA has provided information on the geographic distribution, origin, and evolution of JE viruses. Chen and others found genetic divergence in the C/preM region and arbitrarily chose 12% as a cut-off point to discriminate JE viral isolates into four genotypic groups.…”

Section: Discussionmentioning

confidence: 99%

“…The data suggested that genetic variation occurred among strains collected at different time periods in the same region. [13][14][15][16][17][18] The purpose of the current study was to assess the genetic relationship of JE virus strains isolated in different areas of Taiwan during the period 1983-1994. We examined a 280-nucleotide sequence from the C/preM gene region of the Taiwanese JE virus strains and compared it with those of strains isolated in Asia by direct sequencing of polymerase chain reaction (PCR)-amplified products.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic variation of Japanese encephalitis virus in Taiwan.

Jan

Yueh²,

Wu³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. A 280-nucleotide sequence from the capsid-premembrane (C/preM) gene region of 44 Japanese encephalitis (JE) virus strains isolated in Taiwan from mosquitoes from 1983 to 1994, and 3 strains, (Ling [1965], Chang [1965], and HV1 [1958]) isolated from human brain were analyzed by direct sequencing of reverse transcription-polymerase chain reaction (RT-PCR) amplified products and compared with the corresponding sequences of reference strains. The overall sequence homology of the 47 isolates was Ն 93.3%. Taking 12% nucleotide divergence as a cut-off value, all isolates fell into genotype 3, which included strains from Japan, China, the Philippines, Sri Lanka, India, and Nepal. High nucleotide homology was observed among isolates from different regions of Taiwan and different time periods; on the other hand, high variation existed among isolates from the same region and time period. Phylogenetic analysis showed that the 47 Taiwan isolates fell into three clusters. Twenty-five isolates formed cluster 1, 18 isolates cluster 2, and four isolates cluster 3. Isolates in cluster 1 showed greater (Յ 2.9%) intragroup divergence compared to those in cluster 2 (Յ 1.1%) or cluster 3 (Յ 0.7%). The majority of isolates from northern (73.3%) and central (60%) Taiwan belonged to cluster 1, whereas most isolates (66.7%) from southern Taiwan belonged to cluster 2. Comparison with other Asian JE virus strains showed that isolates of cluster 1 were more specific to Taiwan than isolates of cluster 2 and cluster 3.

show abstract

Genotypes of Japanese Encephalitis Virus Isolated in Three States in Malaysia

Abstract: Abstract.Two

Cited by 29 publications

References 6 publications

Encephalitis and retarded growth of chicks caused by Sitiawan virus, a new isolate belonging to the genus Flavivirus.

Encephalitis and retarded growth of chicks caused by Sitiawan virus, a new isolate belonging to the genus Flavivirus.

Isolation of Japanese encephalitis virus from mosquitoes (Diptera: Culicidae) collected in the Western Province of Papua New Guinea, 1997-1998.

Genetic variation of Japanese encephalitis virus in Taiwan.

Contact Info

Product

Resources

About