Genotypic characterisation and antimicrobial resistance of Pseudomonas aeruginosa strains isolated from patients of different hospitals and medical centres in Poland

Abstract

Background Pseudomonas aeruginosa is a Gram-negative bacteria responsible for infections in immunocompromised patients and is one of the most common causes of nosocomial infections particularly in intensive care and burn units. We aimed to investigate the population structure of P. aeruginosa strains isolated from patients at different hospital wards. Methods: We analysed the possible presence of P. aeruginosa epidemic or endemic strains in hospitals of the selected region. A genotyping analysis was performed for P. aeruginosa isolates (n = 202) collected from patients of eleven hospitals in north-western Poland. Collections of P. aeruginosa were genotyped using pulsed-field gel electrophoresis (PFGE). Phenotypic screening for antibiotic susceptibility was performed for the common antimicrobial agents. Results Pseudomonas aeruginosa isolates were distributed among 116 different pulsotype groups. We identified 30 groups of clonally related strains, each containing from 2 to 17 isolates and typed the obtained 13 unique patterns, designated as A, D, E, J, K, M, N, Ó, P, T, X, AC, AD, and AH. The two largest clusters, D and E, contained 17 and 13 isolates, respectively. Strains of these groups were continuously isolated from patients at intensive care units and burn units, indicating transmission of these strains. Conclusions In this study, we demonstrate the clonal relatedness of P. aeruginosa strains and their constant exchange in hospitals over a period of 15 months. The obtained results indicate a predominantly non-clonal structure of P. aeruginosa.

Occurrence of plasmid-mediated quinolone resistance genes in Pseudomonas aeruginosa strains isolated from clinical specimens in southwest Iran: a multicentral study

“…Previous studies from Iran have approved the high resistance rates of the P. aeruginosa to a wide range of antibiotics, which was similar to current results 19 , 20 . In contrast to these findings, Brzozowski et al 21 from Poland reported a lower resistance rates for ciprofloxacin (39.1%), amikacin (30.7%), cefepime (42.6%), ceftazidime (33.2%), gentamycin (37.6%), piperacillin/tazobactam (39.6%), tobramycin (38.1%), and imipenem (67.8%) in clinical P. aeruginosa . These discrepancies could be due to differences in the geographical regions and diversity of antibiotic prescription patterns, as well as the lack of a comprehensive and organized monitoring program for the proper use of antibiotics in several countries.…”

Isolation and characterization of lytic bacteriophages against Pseudomonas aeruginosa isolates from human infections in the north-west of Iran

“…In another study, isolates of P. aeruginosa revealed 50%, 63.9%, 16.7%, 8.3%, and 16.7% resistance to imipenem, cefepime, amikacin, tobramycin, gentamicin, respectively which is lower than the current study ( 31 ). Brzozowski et al reported lower rate of resistance to imipenem and cefepime and higher rate of resistance to tobramycin, amikacin, and gentamicin ( 32 ). Recently, phage therapy was studied as one of the renewed biologic approaches to combat multi-drug resistant bacteria in therapeutic fields.…”

Exogenous lipoid pneumonia associated with oil-based oral and nasal products

“…The history of P. aeruginosa and S. marcescens cultured bronchoscopy in Poland is surprising as these are generally nosocomial respiratory infections. 1,2 The patient did not have risk factors for communityacquired pneumonia with P. aeruginosa or other gram-negative bacteria, such as pulmonary disease or previous hospital admission, antibiotic use 3 or oral steroid use.…”