Genotypic characterization of Streptococcus mutans in child-mother pair—A PCR based study

Ravikumar, Dhanalakshmi; Ramakrishnan, Mahesh; Ningthoujam, Sharna; Robindro, Waikhom; Gayathri, R.; Priya, V. Vishnu

doi:10.1016/j.jobcr.2017.09.002

Cited by 7 publications

(7 citation statements)

References 23 publications

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“…25,26 According to many studies conducted, there is a positive correlation between the amounts of S. mutans in a mother and a child’s oral flora. 2,17,27 The findings of our study are also consistent with these findings.…”

Section: Discussionsupporting

confidence: 92%

Vertical and Horizontal Transmission of Streptococcus Mutans and Effective Factors: An In Vivo Study

Kızılcı

Özalp

Yılmaz

et al. 2020

Journal of Advanced Oral Research

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Aim: The degree of vertical (intrafamilial) and horizontal (extrafamilial) transfer of Streptococcus mutans ( S. mutans) to a child shows differences according to cultures. The wrong care habits may result in transfer of S. mutans. The aim of this study was to determine the vertical and horizontal transmission of S. mutans to a child and effective factors. Materials and Methods: Mothers and kindergarten staff of 37 children aged between 4 and 5 years who initially started in two kindergartens (Group 1, n = 19; Group 2, n = 18) were included in this in vivo study according to 95% reliability and 80% theoretical power. Intraoral examination of mothers and children made according to the World Health Organization criteria (dmf-t/DMF-T) was done, following which mothers were asked to fill questionnaire forms including knowledge and attitudes of them about oral/dental health care. 1 mL saliva samples received from children/mothers/staff were examined microbiologically in order to isolate and quantify S. mutans. Arbitrarily primed polymerase chain reaction analysis was done to determine the genetic characteristics of S. mutans. All examinations were repeated to evaluate the horizontal transmission in the fifth month. Parametric tests ( t-test and two-way analysis of variance) were employed for comparison of the variables that meet the normality assumption and nonparametric tests (Mann–Whitney) were also used. Results: It was observed that there is a statistically significant correlation between mother DMF-T values and mother–child S. mutans similarity level ( P < .05). There is a statistically positive correlation between the habits of using a common fork, spoon, glass, and mother–child similarity level. Conclusion: Vertical and horizontal transfers occurred in Turkish families in order to prevent the transfer of S. mutans; wrong baby care habits that may lead to the transfer of S. mutans, which is the main factor in case of tooth decay, should be avoided.

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Section: Discussionsupporting

confidence: 92%

Vertical and Horizontal Transmission of Streptococcus Mutans and Effective Factors: An In Vivo Study

Kızılcı

Özalp

Yılmaz

et al. 2020

Journal of Advanced Oral Research

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“…The procedure for DNA extraction was based on the study by Ravikumar et al [ 17 ]. From each subject, about three colonies that resembled S mutans were transferred to brain heart infusion (BHI) broth (Difco, USA).…”

Section: Methodsmentioning

confidence: 99%

Estimating Genotypic Diversity of Streptococcus mutans Isolated From Caries-Active and Caries-Free Individuals Among Indian Population

Poorni¹,

Nivedhitha²,

Srinivasan³

et al. 2022

Cureus

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To identify the genetic characterization of S mutans strains isolated from the caries-free and caries-active population using arbitrarily primed -polymerase chain reaction (AP-PCR). Materials and methodsThirty-five subjects with a decayed missing and filled teeth (DMFT) score of 0 were allocated to the cariesfree group and 35 subjects with a DMFT score greater than four were allocated to the caries-active group and salivary samples were collected. The samples were processed and the colony forming units (CFU) of S mutans were determined for all the samples. About three colonies resembling S mutans from each subject were subjected to deoxyribonucleic acid (DNA) isolation, a purification process was performed, and DNA was analyzed by AP-PCR. ResultsAmong the 210 isolates from 35 caries-active and 35 caries-free subjects that were analyzed by AP-PCR, 41.9% had a single genotype, 25.8% had two genotypes, 19.4% had three genotypes, 9.7% had four genotypes and 3.2% had more than four genotypes among the caries-free group. Among the caries-active group, 54.3% had a single genotype, 25.7% had two genotypes, 14.3% had three genotypes and 5.7% had four genotypes. ConclusionThe study concluded that different genotypic pattern was present in caries-free and caries-active subjects which indicate the occurrence of genetic polymorphism among the S mutans strains. There was no particular genotype of S mutans that was uniquely present in subjects in caries-active and caries-free individuals.

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“…Studies indicate that the main cariesforming bacterial species -Streptococcus mutans, can be transmitted from the caregiver to the child through a contact with saliva. Mothers are most likely to be the main source of a bacterial infection (5).…”

Section: Introductionmentioning

confidence: 99%

“…Badania wskazują na to, że główny próchnicotwórczy gatunek bakterii -Streptococcus mutans może być przenoszony od opiekuna na dziecko poprzez kontakt ze śliną. Najprawdopodobniej to przede wszystkim matki są głównym źródłem infekcji bakteryjnej u swoich dzieci (5).…”

Section: Introductionunclassified

Reasons for dental visits of children and their parents at the dental clinics in Cracow

2022

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INTRODUCTION. Caries is a social disease that affects both children and adults. Parents have a significant influence on the formation of pro-health habits and the state of their children’s oral health. AIM OF THE PAPER. The main aim of the study was to assess the reasons why children and their parents come to the dental office. In addition, the condition of the teeth, the type of performed procedures and the correlation of the oral health between children and their parents were assessed. MATERIAL AND METHODS. During the study, 162 patient’s charts were analyzed from two different dental offices in Crakow. There were 81 parent-child pairs (one child per parent) who were treated in the same dental office between 2004 and 2018. During the first patient visit (parent or child) in the office, the reason for the visit and condition of patient teeth were evaluated. This analysis was based on the dental diagram using the DMF/dmf (Decay-Missing-Filled) Index. Data regarding performed medical procedures during each office visit were collected and documented. RESULTS. In most cases, children came to the dental clinic because of a toothache or cavities caused by caries (75.3%). None of children were ever seen in the dental office for an initial adaptation visit under the age of two. During the first visit, caries was confirmed in 72% of children and 95% of parents. Correlation between child’s DMF/DMF+dmf/dmf and its parent’s DMF indicated a positive linear relationship between these two variables (r = 0.348, p = 0.001). The increase in the total number of children visits to the dental clinic was associated with the increase in the number of visits of their parents (r = 0.327, p = 0.003). The most often performed medical procedures were check-ups (98.8% children and 53.1% parents) and fillings of the permanent teeth (65.4% children and 85.2% adults). CONCLUSIONS. The majority of patients came to the dental clinic because of caries and its complications, not prophylactic procedures. Condition of the tooth hard tissue for both children and parents was less than satisfactory. There is a positive linear correlation between the state of patient dental health and the regularity and frequency of visits to the dental clinic for children and their parents.

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Genotypic characterization of Streptococcus mutans in child-mother pair—A PCR based study

Cited by 7 publications

References 23 publications

Vertical and Horizontal Transmission of Streptococcus Mutans and Effective Factors: An In Vivo Study

Vertical and Horizontal Transmission of Streptococcus Mutans and Effective Factors: An In Vivo Study

Estimating Genotypic Diversity of Streptococcus mutans Isolated From Caries-Active and Caries-Free Individuals Among Indian Population

Reasons for dental visits of children and their parents at the dental clinics in Cracow

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