Genotypic, physiological and biochemical features of Desulfovibrio strains in a sulfidogenic microbial community isolated from the soil of ferrosphere

Abstract

The purpose of this work was the isolation of the predominant representatives of sulfate-reducing bacteria (SRB) of the sulfidogenic microbial community separated from the soil ferrosphere and the examination of their morphological, physiological, biochemical and genotypic peculiarities, the evaluation of some physiological processes under co-culturing with their satellite species Anaerotignum propionicum. During the study two isolates of sulfate-reducing bacteria NUChC SRB1 and NUChC SRB2 were obtained from sulfidogenic microbial community isolated from soil ferrosphere on Postgate's "B" medium and their belonging to different strains (using ISSR-PCR method) was proved. As a result of molecular-genetic analysis of the strains, a 16S rRNA gene fragments of 613 bp and 522 bp were amplified and sequenced. The strains were identified as Desulfovibrio oryzae by the complex of microbiological, physiological and biochemical features and on the basis of 16S rRNA gene sequences (phylogenetic analysis). The 16S rRNA gene sequences were submitted in GenBank as MT102713 (NUChC SRB1) and MT102714 (NUChC SRB2). The co-cultivation of isolated SRB strains with A. propionicum NUChC Sat1 strain (in the absence of electron donors, the presence of sulfates and yeast extract) showed the formation of sulfur-reducing bacteria of hydrogen sulfide, which was not observed during their mono-cultivation. In this case, the phenomenon of syntrophy probably takes place-co-growth on the nutrient substrate, and the electron donor appears due to the use of the yeast extract compounds by the NUChC Sat1 strain. Therefore, in the sulfidogenic community isolated from the soil ferrosphere, there is a mutual growth of the association of bacteria D. oryzae and A. propionicum, which is caused by trophic interaction. Possibly the contribution of these associated bacteria to the corrosion process lies in the utilization of hydrogen (D. oryzae) and the formation of substrate products of SRB metabolism (hydrogen and organic acids), which are both corrosive compounds (A. propionicum). Without a doubt the corrosion process involving this association needs further investigation.ferrosphere, sulfate-reducing bacteria, Desulfovibrio oryzae, 16S rRNA gene, ISSR-PCR.

Research Advances of Soil Corrosion of Grounding Grids

“…The grounding material at a location with a high salt concentration has a lower potential, and the potential of the grounding material at a location with a low salt concentration is higher, which will constitute a galvanic corrosion cell, and the grounding material with low potential will act as anode to accelerate corrosion [ 56 , 57 ]. If the soil contains organic acids or sulfides, the macro cell corrosion can also occur due to changes of soil properties [ 12 , 32 , 33 , 58 , 59 ].…”

Structural and Functional Peculiarities of Outer and Central Cell Layers of Yeast Colony

“…The samples of central and outer cell layers were picked up with tooth sticks and frozen at -20°C before processing. Genomic DNA isolation and amplification with primers to short nucleotide repeats ((GA)9C; (AC)8C; (AG)8) were carried out as described in [3]. RNA was isolated with Trizol reagent, RT-qPCR was performed on qTower 2.2 thermal cycler (Analytik Jena AG, Germany).…”

Biofilms of Some Anaerobic Bacteria on the Polyethylene Terephthalate Surface

“…Five-day pure cultures of A. propionicum NUChC Sat1, D. oryzae NUChC SRB1 and D. oryzae NUChC SRB2 isolated from the sulfidogenic microbial community of soil ferrosphere were used for the research [4][5]. The nucleotide sequences were deposited in the GenBank with accession numbers MG924854.1, MT102713.1 and MT102714.1 respectively.…”

“…Among the microorganisms isolated from the plastic surface, there are representatives of sulfate-reducing bacteria [2]. Previously, anaerobic bacteria were identified as participants in the damage of the metal construction in the soil: organic acid-producing bacteria Anaerotignum propionicum [4] and sulfate-reducing bacteria Desulfovibrio oryzae [5], whose ability to form a biofilm on the surface of PET has not been studied.…”