Genotypic Variation in Geographical Isolates of Oryctes Baculovirus

Crawford, A. M.; Zelazny, B.; Alfiler, A. R.

doi:10.1099/0022-1317-67-5-949

Cited by 27 publications

(15 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…12 isolates have been reported, with some even co-existing within the same geographical area e.g. Luzon, Philippines [6]. And in the Maldive Islands, 3 isolates were released, & after 4 years, 3 new types of genomic change were recovered [17].…”

Section: Commentary and Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Possibility of Evolution in Culture of the Oryctes Nudivirus of the Coconut Rhinoceros Beetle Oryctes rhinoceros (Coleoptera: Scarabaeidae: Dynastinae)

Bedford¹

2018

View full text Add to dashboard Cite

It is suggested that the isolates of Oryctes Nudivirus (OrNV), cultured for decades in cells of Heteronychus arator (F.) (HA), be checked to verify genomic changes have not occurred which adapt them to culture but reduce or cancel their ability to infect the target pest, the coconut rhinoceros beetle (CRB), Oryctes rhinoceros (L.). Full genomes of field-caught OrNV isolates, and their infectivity against larvae and adults, could be compared with those of HA-cultured isolates. Further data to correlate OrNV dosage indices with doses in number of virions/ml could be advantageous so as to explore if CRB larvae or adults may resist infection by a sub-threshold dose. Also the possibility of changes in the HA culture cells which alter the outer coat of the resulting virion, hence perhaps its infectivity towards CRB cells, could be checked. Might it be possible to move beyond HA-culture and develop tissue culture of Oryctes rhinoceros cells for mass production of OrNV as this beetle species is the target? Nuclear genomes of OrNV-resistant and OrNV-susceptible strains of the CRB could be examined for changes perhaps correlated with resistance. The possibility of endosymbiotic bacteria affecting CRB susceptibility to OrNV might be checked.

show abstract

Section: Commentary and Discussionmentioning

confidence: 99%

“…in India [4] [5], the Philippines [6], and Malaysia [7]. The OrNV virion is the infective unit which must survive in the environment and be transmitted naturally or by artificial dosages [1] [2] [8].…”

Section: Introductionmentioning

confidence: 99%

Possibility of Evolution in Culture of the Oryctes Nudivirus of the Coconut Rhinoceros Beetle Oryctes rhinoceros (Coleoptera: Scarabaeidae: Dynastinae)

Bedford¹

2018

View full text Add to dashboard Cite

show abstract

“…X2B is a highly virulent strain originally isolated in 1983 from a field population of infected coconut rhinoceros beetles on Bugsuk Island, Palawan, Philippines (Crawford et al 1986;Zelazny et al 1990).…”

Section: Virus and Virus Quantificationmentioning

confidence: 99%

Characterization of growth and Oryctes rhinoceros nudivirus production in attached cultures of the DSIR-HA-1179 coleopteran insect cell line

et al. 2013

View full text Add to dashboard Cite

The DSIR-HA-1179 coleopteran cell line is a susceptible and permissive host to the Oryctes rhinoceros nudivirus (OrNV), which has been used as a biocontrol agent against the coconut rhinoceros beetle (Oryctes rhinoceros); a pest of palms in the Asia-Pacific region. However, little is known about growth and metabolism of this cell line, knowledge of which is necessary to develop an in vitro large-scale OrNV production process. The strong anchoragedependent characteristics of the cell line, its particular fragility and its tendency to form dense clumps when manipulated, are the most likely reasons that have precluded further development of the cell line. In order to characterize DSIR-HA-1179 cells, there was first a need for a reliable technique to count the cells. A homogenous cell suspension suitable for enumeration could be produced by treatment with TrypLE Express TM with optimum mean time for cell release calculated as 30 min. The cell line was adapted to grow in four serum-supplemented culture media namely TC-100, IPL-41, Sf-900 II and Sf-900 III and cell growth, glucose consumption, lactate and ammonia production were assessed from static-batch cultures. The maximum viable cell density was reached in Sf-900 II (17.9 9 10 5 cells/ml), with the maximum specific growth rate observed in this culture medium as well (0.0074 h -1 ). Higher production of OrNV was observed in IPL-41 and TC-100 (4.1 9 10 7 TCID 50 /ml) than in cultures infected in Sf-900 III (2.0 9 10 7 TCID 50 /ml) and Sf-900 II (1.4 9 10 7 TCID 50 /ml). At the end of the growth period, glucose was completely consumed in cultures grown in TC-100, while remained in excess in the other three culture media. The cell line produced lactate and ammonia to very low levels in the TC-100 culture medium which is a promising aspect for its cultivation at large-scale.

show abstract

“…Mohan and K. P. Gopinathan biological control of rhinoceros beetles in field trials [ M o h a n and Pillai, unpublished observations] and is of considerable economic importance. Oryctes baculovirus has also been isolated from the wild population of O. rhinoceros in Seychelles and the islands of Indonesia and the Philippines [11,33].…”

Section: Introductionmentioning

confidence: 99%

Characterization of viral proteins ofOryctes baculovirus and comparison between two geographical isolates

Mohan

Gopinathan

1989

Archives of Virology

View full text Add to dashboard Cite

Bacilliform Oryctes baculovirus particles have been visualized in electron micrographs of midgut sections from virus infected Oryctes rhinoceros beetles. Morphologically the Indian isolate (Oryctes baculovirus, KI) resembled the previously reported Oryctes baculovirus, isolate PV505. The constituent proteins of baculovirus KI have been analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and by Western blots using polyclonal antibodies raised against the complete viral particles, as probes. A total of forty eight viral proteins have been identified. Fourteen viral proteins were located on the viral envelope. Among the proteins constituting the nucleocapsid, three were located internally within the capsid. A 23.5 kDa protein was tightly associated with viral DNA in the nucleocapsid core. Two envelope and seven capsid proteins of KI and PV505 revealed differences in SDS-PAGE profiles and glycosylation patterns. Immunoblotting of KI and PV505 proteins with anti KI antiserum demonstrated antigenic differences between the two viral isolates.

show abstract

Genotypic Variation in Geographical Isolates of Oryctes Baculovirus

Cited by 27 publications

References 5 publications

Possibility of Evolution in Culture of the <i>Oryctes</i> Nudivirus of the Coconut Rhinoceros Beetle <i>Oryctes rhinoceros</i> (Coleoptera: Scarabaeidae: Dynastinae)

Possibility of Evolution in Culture of the <i>Oryctes</i> Nudivirus of the Coconut Rhinoceros Beetle <i>Oryctes rhinoceros</i> (Coleoptera: Scarabaeidae: Dynastinae)

Characterization of growth and Oryctes rhinoceros nudivirus production in attached cultures of the DSIR-HA-1179 coleopteran insect cell line

Characterization of viral proteins ofOryctes baculovirus and comparison between two geographical isolates

Contact Info

Product

Resources

About

Genotypic Variation in Geographical Isolates of Oryctes Baculovirus

Cited by 27 publications

References 5 publications

Possibility of Evolution in Culture of the &lt;i&gt;Oryctes&lt;/i&gt; Nudivirus of the Coconut Rhinoceros Beetle &lt;i&gt;Oryctes rhinoceros&lt;/i&gt; (Coleoptera: Scarabaeidae: Dynastinae)

Possibility of Evolution in Culture of the &lt;i&gt;Oryctes&lt;/i&gt; Nudivirus of the Coconut Rhinoceros Beetle &lt;i&gt;Oryctes rhinoceros&lt;/i&gt; (Coleoptera: Scarabaeidae: Dynastinae)

Characterization of growth and Oryctes rhinoceros nudivirus production in attached cultures of the DSIR-HA-1179 coleopteran insect cell line

Characterization of viral proteins ofOryctes baculovirus and comparison between two geographical isolates

Contact Info

Product

Resources

About

Possibility of Evolution in Culture of the <i>Oryctes</i> Nudivirus of the Coconut Rhinoceros Beetle <i>Oryctes rhinoceros</i> (Coleoptera: Scarabaeidae: Dynastinae)

Possibility of Evolution in Culture of the <i>Oryctes</i> Nudivirus of the Coconut Rhinoceros Beetle <i>Oryctes rhinoceros</i> (Coleoptera: Scarabaeidae: Dynastinae)