Genotyping and antifungal susceptibility testing of <i>Sporothrix brasiliensis</i> isolates from Southern Brazil

Bombassaro, Amanda; Spruijtenburg, Bram; Medeiros, Fernanda; Lima, Bruna Jacomel Favoreto de Souza; Ballardin, Luana Beatriz; Farias, Marconi Rodrigues de; Vicente, Vânia Aparecida; Queiroz‐Telles, Flávio; Meis, Jacques F.; Groot, Theun de

doi:10.1111/myc.13584

Cited by 11 publications

(7 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…where the genetic diversity in the S. brasiliensis population observed in human and animal outbreaks suggests independent introductions of the species over time. 58,61 These data reinforce the results observed in the study. The…”

Section: Discussionsupporting

confidence: 90%

See 1 more Smart Citation

Emergence of zoonotic sporotrichosis due to Sporothrix brasiliensis in Minas Gerais, Brazil: A molecular approach to the current animal disease

Colombo

Bicalho

Oliveira

et al. 2023

Mycoses

View full text Add to dashboard Cite

Sporotrichosis is a neglected fungal zoonosis with significant impacts on human and animal health. Accurate diagnosis, treatment, and understanding of the transmission dynamics of Sporothrix species are essential for mitigating the spread of sporotrichosis. This study aimed to identify the Sporothrix species involved in the ongoing outbreaks of animal sporotrichosis in the metropolitan region of Belo Horizonte, Minas Gerais, Brazil, and analyse the phylogenetic relationships between pathogenic species to investigate the outbreak origin. Additionally, to better understand the evolution of the disease, we conducted a retrospective survey of positive feline and canine cases from November 2017 to July 2021 with proven cultures for Sporothrix. A significant increase in animal cases over the last 4 years was observed, with cats being the most affected host. Sporothrix brasiliensis was the predominant agent in 100% of the clinical isolates (n = 180) molecularly identified. Phylogenetic and haplotype analysis points towards the cases isolated from Minas Gerais sharing the haplotype originating from a long‐lasting outbreak of cat‐transmitted sporotrichosis in Rio de Janeiro, however, with a secondary contribution from genotypes circulating in other outbreaks in Brazil. Thus, we present clear evidence of the circulation of different S. brasiliensis genotypes associated with animal sporotrichosis in the metropolitan region of Belo Horizonte. Genetic monitoring can contribute to understanding the causal agent for zoonotic sporotrichosis in epidemiological processes and help to implement disease prevention and control measures.

show abstract

Section: Discussionsupporting

confidence: 90%

“…Amplified fragment length polymorphism markers and simple sequence repeats (SSRs) revealed cryptic diversity in S. brasiliensis, which is directly associated with the distribution of sexual idiomorphs, 17,[56][57][58] revealing a critical phylogeographic structure in S.…”

Section: Discussionmentioning

confidence: 99%

Emergence of zoonotic sporotrichosis due to Sporothrix brasiliensis in Minas Gerais, Brazil: A molecular approach to the current animal disease

Colombo

Bicalho

Oliveira

et al. 2023

Mycoses

View full text Add to dashboard Cite

show abstract

“…Olorofim was also tested against other dimorphic fungi such as Sporothrix brasiliensis by Bombassaro et al and Coccidioides spp. by Wiederhold et al [22,23]. For S. brasiliensis, the GMIC was 0.026 mg/L among 52 strains; as for Coccidioides spp., the GMIC was 0.011 mg/L among 59 strains in total.…”

Section: In Vitro Studiesmentioning

confidence: 83%

The Role of Olorofim in the Treatment of Filamentous Fungal Infections: A Review of In Vitro and In Vivo Studies

Feuss,

Bougnoux,

Dannaoui

2024

JoF

View full text Add to dashboard Cite

Invasive fungal infections have recently been recognized by the WHO as a major health, epidemiological, and economic issue. Their high mortality rates and the emergence of drug resistance have driven the development of new molecules, including olorofim, an antifungal belonging to a new family of compounds, the orotomides. A review was conducted on the PubMed database and the ClinicalTrials.gov website to summarize the microbiological profile of olorofim and its role in the treatment of filamentous fungal infections. Twenty-four articles were included from the search and divided into two groups: an “in vitro” group focusing on minimum inhibitory concentration (MIC) results for various fungi and an “in vivo” group evaluating the pharmacokinetics (PK), pharmacodynamics (PD), efficacy, and tolerability of olorofim in animal models of fungal infection and in humans. Olorofim demonstrated in vitro and in vivo activity against numerous filamentous fungi, including azole-resistant Aspergillus fumigatus, various dermatophytes, and endemic and dimorphic fungi. in vitro results showed higher MICs for certain Fusarium species and dematiaceous fungi Alternaria alternata and Exophiala dermatitidis; further in vivo studies are needed. Published PK-PD data in humans are limited. The results of the ongoing phase III clinical trial are eagerly awaited to evaluate olorofim’s clinical impact.

show abstract

“…a collaborative, multisectoral, and transdisciplinary approach for veterinary, public health and environmental sectors. In Brazil, during the period from 2011 to 2022, 13,475 cases of human sporotrichosis were reported in the metropolitan region of Rio de Janeiro and cases are currently reported in most states [ 10 , 11 ]. Between 2011 and 2014, the first report of zoonotic sporotrichosis in Argentina involved four cat-related cases of human sporotrichosis in Buenos Aires [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Genotyping and antifungal susceptibility testing of Sporothrix brasiliensis isolates from Southern Brazil

Cited by 11 publications

References 73 publications

Emergence of zoonotic sporotrichosis due to Sporothrix brasiliensis in Minas Gerais, Brazil: A molecular approach to the current animal disease

Emergence of zoonotic sporotrichosis due to Sporothrix brasiliensis in Minas Gerais, Brazil: A molecular approach to the current animal disease

The Role of Olorofim in the Treatment of Filamentous Fungal Infections: A Review of In Vitro and In Vivo Studies

Genotyping and clonal origin of Sporothrix brasiliensis in human sporotrichosis cases in Argentina

Contact Info

Product

Resources

About