Genotyping Approaches for Identification and Characterization of Staphylococcus aureus

Abstract

Genotyping methods are vital epidemiological tools for discriminating different bacterial isolates within same species, which in turn provide useful data in tracing source of infection and disease management. There have been a revolutionary efforts in ways to distinguish between bacterial types and subtypes at molecular level utilizing DNA in the genomes. Notably, the growth of various DNA typing methods has provided innovative apparatuses for improved surveillance and outbreak investigation. Thus, early identification and genotyping are indispensable as resources for managing therapeutic treatment and the control of rapid expansion of clinically important bacteria. Methicillin-resistant Staphylococcus aureus (MRSA) has been in a great attention due to its contagious nature and subjected to various typing analyses. Thus, in this chapter, we aimed to review the contribution of various genotyping methods of commonly used as well as those unique to staphylococci in understanding its epidemiology, infection and dissemination pattern, and to provide an impression of specific advantages and disadvantages of each tool.

Methicillin-Resistant Staphylococcus aureus ST80 Clone: A Systematic Review

“…Molecular typing methods constitute invaluable tools for tracing the spread of particular strains, discovering the route of dissemination and the potential reservoirs. Furthermore, molecular typing contributes to the comprehensive understanding of the epidemiology of infection and facilitates infection control measures, as well as management [120]. From the analyzed articles included in this review, several methods were used for MRSA typing.…”

Review of pork and pork products as a source for transmission of methicillin-resistant Staphylococcus aureus

“…Molecular methods used to examine MRSA have been widely used in several places [80,81]. The standard method used to detect human MRSA (CA-MRSA) is gel electrophoresis [82].…”

First insights into molecular basis identification of 16 s ribosomal RNA gene of Staphylococcus aureus isolated from Sudan

“…Additionally, using (16S rRNA) as individual indication is regarding difficult to evaluate the bacterial diversity and identifying bacterial species [ 45 ], subsequently, the resolution of 16S rRNA that is extremely restricted with relative species but not quite characteristic to distinguish correlated staphylococcal spp. Finally, the highly conserved genomes may not have enough DNA polymorphisms in these restricted sequence proposed to display alleles [ 46 ].…”