Gentamicin and amikacin adversely affect male infertility indicated by pharmacological, andrological and pathological evidence

Elsawah, Hozaifa; Kandiel, Mohamed M.M.; Amin, Alaa S.; Mokhimar, Haitham; Mahmoudy, AbuBakr M. El

doi:10.18203/2319-2003.ijbcp20200167

Cited by 6 publications

(13 citation statements)

References 17 publications

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“…The serum LH and FSH levels decreased after PIO treatment. These results were in line with other previous studies which found that serum testosterone level was significantly decreased in gentamycin-treated groups [ 6 , 38 , 42 , 57 ]. The serum FSH and LH levels were both significantly elevated in all gentamycin-treated groups [ 38 ].…”

Section: Discussionsupporting

confidence: 93%

“…These results were in line with other previous studies which found that serum testosterone level was significantly decreased in gentamycin-treated groups [ 6 , 38 , 42 , 57 ]. The serum FSH and LH levels were both significantly elevated in all gentamycin-treated groups [ 38 ]. On the other hand, the serum FSH level was measured in an experimental study that examined the effects of gentamycin.…”

Section: Discussionsupporting

confidence: 93%

“…This was associated with a decrease in Johnsen’s score to 3. Hand in hand with our study, several studies found histopathological signs of testicular cell loss together with germ cell loss [ 6 , 38 , 42 , 43 ].…”

Section: Discussionsupporting

confidence: 88%

“…In the present study, the gentamycin group resulted in altered sperm parameters such as a significant decrease in the count, motility, and viability of the sperm compared to the control group ( p < 0.01) with significant reduction of Johnsen’s score to 3. This negative effect of gentamycin on sperm parameters was also reported by others [ 3 , 6 , 38 , 40 ]. Reduction in sperm parameters due to gentamycin treatment could be attributed to a decreased level of serum testosterone, production of sperms in testis, and maturation of sperms in epididymis.…”

Section: Discussionsupporting

confidence: 86%

“…These findings are also confirmed with a study using doses of 60, 80, and 100 mg/kg for 10 days ( p < 0.05), which reported a significant reduction in relative testicular weight with the higher two doses compared to control groups ( p < 0.05) [ 6 ]. Several studies reported the same effect on testicular weight [ 38 , 39 ]. Another study used gentamycin in a dose of 100 mg/kg/day for six days and found no significant difference in testis and seminal vesicle weight ( p > 0.05) [ 40 ].…”

Section: Discussionmentioning

confidence: 84%

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Prophylactic and Ameliorative Effects of PPAR-γ Agonist Pioglitazone in Improving Oxidative Stress, Germ Cell Apoptosis and Inflammation in Gentamycin-Induced Testicular Damage in Adult Male Albino Rats

et al. 2022

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Peroxisome proliferator-activated receptor gamma (PPAR-γ) is ubiquitously expressed in testicular tissue and plays a crucial role in regulating various physiological processes. Pioglitazone (PIO) is one of the PPAR-γ agonists, having anti-oxidant and anti-inflammatory effects. Patients on gentamycin treatment may undergo serious side effects such as testicular damage. To the best of our knowledge, this was the first study to investigate the possible protective anti-inflammatory and anti-apoptotic effects of PIO on gentamycin-induced testicular damage. Fifty adult male Wistar albino rats included in the study as the control group (CTL) received normal saline; a gentamycin-induced testicular damage group (GM) received gentamycin (100 mg/kg); PIO5, PIO10, PIO20 groups received PIO at a dose of 5, 10, and 20 mg/ kg, respectively, for 21 days, and gentamycin was started at day 15 of the experiment for 6 days. The parameters of spermatozoa and histopathological alterations in the testes were significantly improved in the PIO20 group. Moreover, MDA levels, inflammatory mediators, and apoptotic Bax expression were decreased. The activity of glutathione peroxidase, catalase, total antioxidant capacity, and anti-apoptotic Bcl-2 genes expression were increased. It was concluded that PIO20 could protect against gentamycin-induced testicular damage in Wistar rats through its anti-oxidant, anti-inflammatory, and antiapoptotic effects.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 84%

See 3 more Smart Citations

Prophylactic and Ameliorative Effects of PPAR-γ Agonist Pioglitazone in Improving Oxidative Stress, Germ Cell Apoptosis and Inflammation in Gentamycin-Induced Testicular Damage in Adult Male Albino Rats

et al. 2022

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Assessment ameliorative role of fenugreek seeds and germinated fenugreek seeds on pancreatic and testicular gentamicin toxicity of male Swiss albino mice

Hamad

Anwar

Diab

2023

JoBAZ

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Background Gentamicin is one of aminoglycoside antibiotic used for treatment of many infections due to its availability and less cost. The aim of this study aimed to assess the modulation effect of fenugreek seed and its germinated seeds on pancreatic and testicular toxicity induced by gentamicin in male Swiss albino mice. Forty male albino mice were divided into four treatment groups as follows: (1) control group, (2) gentamicin treated group, (3) gentamicin-fenugreek treated group and (4) gentamicin-germinated fenugreek treated group. Pancreatic and testicular tissues were collected for histopathological examinations, histochemical, and biochemical analysis as well as genetic study. Results Administration of gentamicin resulted in histopathological damage in pancreatic and testicular tissues as well as decreased glutathione peroxides, catalase and total antioxidant activity content in both pancreatic and testicular tissues compared to control group. Histopathological changes and antioxidant/oxidative alterations as well as DNA damage observed in gentamicin treated animals found were moderate improvement by fenugreek seeds administration and marked improvement by treatment with germinated fenugreek seeds. Conclusions Treated with gentamicin induced histopathological lesions, antioxidant/oxidant imbalance and DNA damage in the pancreatic and testicular. Treatment with germinated fenugreek seeds was more effective than fenugreek seeds in amelioration of pancreatic and testicular lesions, preventing high appearance of carbohydrate and accumulation of collagen fibers as well as oxidative damage and genotoxicity induced by gentamicin administration.

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The ameliorative effect of α-lipoic acid on testicular dysfunction induced by gentamicin.

Elsawah

Mokhimar²,

Kandiel³

et al. 2022

Benha Veterinary Medical Journal

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Drug exposure is a common risk factor for male infertility and should be investigated carefully. This study aimed to evaluate the possible protective effect of α-lipoic acid against gentamicininduced-testicular damage. Sixty Wister male rats aged 8 weeks and weighted 200±20 gm were randomly assigned to six groups of ten. Groups 1 and 2 were treated with 18.25 and 36.5mg/kg gentamicin, respectively. Groups 3, 4, and 5 were treated with 36.5mg/kg gentamicin plus 100mg/Kg/day α-lipoic acid (ALA), 200mg/Kg/day ALA, and 100mg/kg vitamin E, respectively. Group 6 served as control. All treatments were administered once daily for 14 days. Half the animals in each group were sacrificed on the 15th day, while the second half was sacrificed on the 60th day of the experiment. Blood samples were collected from the retroorbital venous plexus just before sacrifice. Immediately after the sacrifice, reproductive organs were removed and weighed, and semen was collected for analysis. Groups treated with gentamicin and either ALA or vitamin E showed preserved reproductive organs weight and sperm parameters compared to gentamicin alone on both sacrifice days (p<0.05). Testosterone level was also preserved with either ALA or vitamin-E co-administrated with gentamicin compared to gentamicin alone (p<0.05). However, compared to gentamicin alone, gonadotropin levels showed variable levels with the cotreatments. The hormone levels of all groups were approximately normalized on the second sacrifice day. Pathological lesions induced by gentamicin were markedly reduced upon cotreatment with ALA. Adding α-lipoic acid or vitamin E to gentamicin therapy protects from gentamicin-induced reproductive system impairment in experimental animals.

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Gentamicin and amikacin adversely affect male infertility indicated by pharmacological, andrological and pathological evidence

Cited by 6 publications

References 17 publications

Prophylactic and Ameliorative Effects of PPAR-γ Agonist Pioglitazone in Improving Oxidative Stress, Germ Cell Apoptosis and Inflammation in Gentamycin-Induced Testicular Damage in Adult Male Albino Rats

Prophylactic and Ameliorative Effects of PPAR-γ Agonist Pioglitazone in Improving Oxidative Stress, Germ Cell Apoptosis and Inflammation in Gentamycin-Induced Testicular Damage in Adult Male Albino Rats

Assessment ameliorative role of fenugreek seeds and germinated fenugreek seeds on pancreatic and testicular gentamicin toxicity of male Swiss albino mice

The ameliorative effect of α-lipoic acid on testicular dysfunction induced by gentamicin.

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