Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov.

Banat, Ibrahim M.; Marchant, Roger; Rahman, Thahira

doi:10.1099/ijs.0.63231-0

Cited by 95 publications

(55 citation statements)

References 19 publications

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“…The fatty acid pattern was also quite different from those of Geobacillus species (Table 2) with C 16 : 0 as the major fatty acid (50.4 %). We conclude that G. debilis is a member of a genus that is separate from Geobacillus, and we supplement the description given by Banat et al (2004) in our proposal to transfer G. debilis to the novel, single-species genus Caldibacillus as Caldibacillus debilis gen. nov., comb. nov.…”

Section: Bacillus Thermantarcticusmentioning

confidence: 78%

“…Nazina et al (2001) proposed that the six species of that lineage, namely B. stearothermophilus, B. kaustophilus, B. thermoglucosidasius, B. thermocatenulatus, B. thermoleovorans and B. thermodenitrificans, should be accommodated in a new genus, Geobacillus, along with two novel species, Geobacillus subterraneus and Geobacillus uzenensis. Subsequently, Bacillus pallidus (Scholz et al, 1987), Saccharococcus caldoxylosilyticus (Ahmad et al, 2000) and Bacillus vulcani (Caccamo et al, 2000) were transferred to Geobacillus (Fortina et al, 2001;Banat et al, 2004;Nazina et al, 2004) and then Miñana-Galbis et al (2010) proposed the further transfer of Geobacillus pallidus to the new genus Aeribacillus. Subsequently, six further species, Geobacillus toebii (Sung et al, 2002), G. gargensis (Nazina et al, 2004), G. debilis , G. lituanicus (Kuisiene et al, 2004), G. tepidamans (Schäffer et al, 2004) and G. jurassicus (Nazina et al, 2005) have been proposed and the names 'Geobacillus caldoproteolyticus' (Chen et al, 2004) and the subspecies 'G.…”

Section: Introductionmentioning

confidence: 99%

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Taxonomic revision of the genus Geobacillus : emendation of Geobacillus , G. stearothermophilus , G. jurassicus , G. toebii , G. thermodenitrificans

Coorevits

Dinsdale

Halket

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

161

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Sixty-two strains of thermophilic aerobic endospore-forming bacteria were subjected to polyphasic taxonomic study including 16S rRNA gene sequence analysis, polar lipid and fatty acid analysis, phenotypic characterization, and DNA-DNA hybridization experiments. Distinct clusters of the species Geobacillus stearothermophilus, Geobacillus thermodenitrificans, Geobacillus toebii and Geobacillus thermoglucosidasius were formed, allowing their descriptions to be emended, and the distinctnesses of the poorly represented species Geobacillus jurassicus, Geobacillus subterraneus and Geobacillus caldoxylosilyticus were confirmed. It is proposed that the name Geobacillus thermoglucosidasius be corrected to G. thermoglucosidans. Bacillus thermantarcticus clustered between Geobacillus species on the basis of 16S rRNA gene sequence analysis, and its transfer to Geobacillus is proposed. The above-mentioned species, together with Geobacillus thermoleovorans and Geobacillus thermocatenulatus, form a monophyletic cluster representing the genus Geobacillus. The distinctness of 'Geobacillus caldoxylosilyticus' was confirmed and it is proposed that it be accommodated, along with Geobacillus tepidamans, in Anoxybacillus. The type strain of Geobacillus debilis was not closely related to any members of Anoxybacillus and Geobacillus, and it is proposed that this species be placed in the new genus Caldibacillus

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Section: Bacillus Thermantarcticusmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Taxonomic revision of the genus Geobacillus : emendation of Geobacillus , G. stearothermophilus , G. jurassicus , G. toebii , G. thermodenitrificans

Coorevits

Dinsdale

Halket

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

161

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“…The isolated bacteria may respond directly to ambient condition or to temporal changes in stimulus intensity as reported by Wu et al (2003). According to literature, Bacillus was gram-variable and endospore-forming bacteria (Banat et al, 2004). It was reported that Bacillus subtillis was moved in a whirl and jet motion neither individually nor in cell groups (Mendelson et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Thermophilic Cellulase-Producing Bacteria from Empty Fruit Bunches-Palm Oil Mill Effluent Compost

Baharuddin

Razak

Hock

et al. 2010

American Journal of Applied Sciences

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Problems statement: Lack of information on locally isolated cellulase-producing bacterium in thermophilic compost using a mixture of Empty Fruit Bunch (EFB) and Palm Oil Mill Effluent (POME) as composting materials. Approach: The isolation of microbes from compost heap was conducted at day 7 of composting process where the mixture of composting materials consisted of 45.8% cellulose, 17.1% hemicellulose and 28.3% lignin content. The temperature, pH and moisture content of the composting pile at day 7 treatment were 58.3, 8.1 and 65.5°C, respectively. The morphological analysis of the isolated microbes was conducted using Scanning Electron Microscope (SEM) and Gram stain method. The congo red test was conducted in order to detect 1% CMC agar degradation activities. Total genomic DNAs were extracted from approximately 1.0 g of mixed compost and amplified by using PCR primers. The PCR product was sequent to identify the nearest relatives of 16S rRNA genes. The localization of bacteria chromosomes was determined by Fluorescence In Situ Hybridization (FISH) analysis. Results: Single isolated bacteria species was successfully isolated from Empty Fruit Bunch (EFB)-Palm Oil Mill Effluent (POME) compost at thermophilic stage. Restriction fragment length polymorphism profiles of the DNAs coding for the 16S rRNAs with the phylogenetic analysis showed that the isolated bacteria from EFB-POME thermophilic compost gave the highest homology (99%) with similarity to Geobacillus pallidus. The strain was spore forming bacteria and able to grow at 60°C with pH 7. Conclusion: Thermophilic bacteria strain, Geobacillus pallidus was successfully isolated from Empty Fruit Bunch (EFB) and Palm Oil Mil Effluent (POME) compost and characterized.

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“…(-) (Nazina et al, 2001), 9: G. caldoxylosilyticus DSM 12041T (Fortina et al, 2001), 10: G. toebii DSM 13551 (Sung et al, 2002), 11: G. gargensis DSM 15378 ve 12: G. vulcani DSM 13174 (Nazina et al, 2004), 13: G. jurassicus DSM 15726T (Nazina et al, 2005), 14: G. debilis DSM 16016T ve 15: G. palidus (Banat et al, 2004), 16: G. lituanicus DSM 15325T (Kuisiene et al, 2004), 17: G. tepidamans (Schaffer et al, 2004), 18: G. thermoantarcticus DSM 9572T (Coorevits et al, 2012), 19: G. galactosidasius DSM 18751 (Poli et al, 2011), 20: G. icigianus (Bryanskaya et al, 2015) Türkiye'deki Dibekli Sıcak Su Kaynağından Termofilik Geobacillus sp. DB2 Suşunun Fizyo-Biyokimyasal Karakterizasyonu ve 16S rRNA Sekanslaması DB2 suşunun yapılan kısmi 16S rRNA sekans analizlerine göre Geobacillus türleri içinde %98.97 benzerlik oranıyla G. subterraneus subsp.…”

Section: şEkil 2a: Db2'nin Kültüvasyon Zamanıunclassified

Physio-Biochemical Characterization and 16S rRNA Sequencing of Thermophilic Geobacillus sp. stain DB2 from Dibekli Hot Spring, Turkey

Bekler¹

2017

jist

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Jeotermal kaynaklar termofillerin habitatlarındandır. Bu çalışmada aerobik, çubuk şeklinde, hareketli ve Gram-pozitif termofilik DB2 suşu Türkiye'deki Diyadin ilçesi Dibekli sıcak su kaynağından izole edilerek morfolojik, fizyolojik, biyokimyasal testler ve 16S rRNA gen sekanslamayla tanımlandı. Optimal kültivasyon pH 6.0-6.5'da, 60 o C'de elde edildi. DB2 suşunun katalaz, nitrat redükleme, Metil red testi ve nişasta hidrolizi pozitif, oksidaz, üreaz aktivitesi, jelatin ve kazein hidrolizi negatif olarak belirlendi. 16S rRNA gen sekansına bağlı filogenetik analizler DB2 suşunun %98.97 sekans benzerliği ile Geobacillus subterraneus subsp. aromaticivorans Ge1(T)'e oldukça yakın olduğunu göstermektedir. Bu yeni tür, biyoteknolojik uygulamalarda kullanılabilir özelliktedir.

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Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov.

Abstract: Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov.

Cited by 95 publications

References 19 publications

Taxonomic revision of the genus Geobacillus : emendation of Geobacillus , G. stearothermophilus , G. jurassicus , G. toebii , G. thermodenitrificans

Taxonomic revision of the genus Geobacillus : emendation of Geobacillus , G. stearothermophilus , G. jurassicus , G. toebii , G. thermodenitrificans

Isolation and Characterization of Thermophilic Cellulase-Producing Bacteria from Empty Fruit Bunches-Palm Oil Mill Effluent Compost

Physio-Biochemical Characterization and 16S rRNA Sequencing of Thermophilic Geobacillus sp. stain DB2 from Dibekli Hot Spring, Turkey

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