Geographical distribution and epidemiological features of Old World<i>Leishmania infantum</i>and<i>Leishmania donovani</i>foci, based on the isoenzyme analysis of 2277 strains

Pratlong, Francine; Lami, Patrick; Ravel, Christophe; Balard, Yves; Dereure, J.; Serres, Ghislaine; Baidouri, Fouad El; Dedet, Jean-Pierre

doi:10.1017/s0031182012001825

Cited by 43 publications

(52 citation statements)

References 44 publications

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“…Out of these, IMT 401 (MCAN/PT/13/IMT401) was sent to the Centre National de Référence des Leishmanioses (Montpellier, France) and typed by multilocus enzyme electrophoresis (MLEE) [14] as L. infantum MON-1, the most common zymodeme in both humans and dogs in the Mediterranean basin [15]. The low sensitivity of cultural parasitological techniques has also been described by other authors [16, 17].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of four molecular methods to detect Leishmania infection in dogs

et al. 2017

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BackgroundCanine leishmaniasis, a zoonotic disease caused by Leishmania infantum vectored by phlebotomine sand flies, is considered a relevant veterinary and public health problem in various countries, namely in the Mediterranean basin and Brazil, where dogs are considered the main reservoir hosts. Not only diseased dogs but also those subclinically infected play a relevant role in the transmission of L. infantum to vectors; therefore, early diagnosis is essential, under both a clinical and an epidemiological perspective. Molecular tools can be a more accurate and sensitive approach for diagnosis, with a wide range of protocols currently in use. The aim of the present report was to compare four PCR based protocols for the diagnosis of canine Leishmania infection in a cohort of dogs from the Douro region, Portugal.ResultsA total of 229 bone marrow samples were collected from dogs living in the Douro region, an endemic region for leishmaniasis. Four PCR protocols were evaluated for Leishmania DNA detection in canine samples, three single (ITS1-PCR, MC-PCR and Uni21/Lmj4-PCR) and one nested (nested SSU rRNA-PCR). Two of the protocols were based on nuclear targets and the other two on kinetoplastid targets. The higher overall percentage of infected dogs was detected with the nested SSU rRNA-PCR (37.6%), which also was able to detect Leishmania DNA in a higher number of samples from apparently healthy dogs (25.3%). The ITS1-PCR presented the lowest level of Leishmania detection.ConclusionsNested SSU rRNA-PCR is an appropriate method to detect Leishmania infection in dogs. Accurate and early diagnosis in clinically suspect as well as apparently healthy dogs is essential, in order to treat and protect animals and public health and contribute to the control and awareness of the disease.

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Section: Resultsmentioning

confidence: 99%

Evaluation of four molecular methods to detect Leishmania infection in dogs

et al. 2017

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“…Leishmania infantum MON-1, found predominantly in the Mediterranean Basin (90% of the typed isolates), 31,48 was *Only data with more than 60 examined dogs per prefecture were taken into account for the analysis of the results. Table 3 Recorded presence of sandflies, Phlebotomus (Figure 2), the rare zymodeme MON-98 was found in patients (24.4%) and dogs (17.1%) but only in the central and southern parts of the country (Figure 2).…”

Section: Discussionmentioning

confidence: 99%

Leishmaniases in Greece

Ntais¹,

Sifaki-Pistola²,

Christodoulou³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. During the last 35 years, visceral leishmaniasis has spread in Greece with autochthonous human cases appearing in 41 of the 54 prefectures. The occurrence of the disease was mapped and related to dog seropositivity, environmental and geospatial risk factors. Average dog seropositivity was 22.1% and positive animals were found in 43 of 54 prefectures. Factors like: altitude, presence of water bodies, land use, wind speed, mean land surface temperature, mean relative humidity, and mean annual rainfall were found to affect dog seropositivity. Cases of cutaneous leishmaniasis, caused by Leishmania tropica are also increasing. Phlebotomus similis believed to be the potential vector of L.

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“…The combined pattern of all these proteins constitutes a so-called zymodeme, which serves as the basis for species assignment as well as for classification below the species level (30,55,58,(86)(87)(88). Nevertheless, different labs make use of other enzymes, leading to several identification systems, such as the MON (Montpellier, France) (26), LON (London, United Kingdom) (89), and IOC (Instituto Oswaldo Cruz, Rio de Janeiro, Brazil) (90) classifications.…”

Section: Mleementioning

confidence: 99%

Species Typing in Dermal Leishmaniasis

2015

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SUMMARY Leishmania is an infectious protozoan parasite related to African and American trypanosomes. All Leishmania species that are pathogenic to humans can cause dermal disease. When one is confronted with cutaneous leishmaniasis, identification of the causative species is relevant in both clinical and epidemiological studies, case management, and control. This review gives an overview of the currently existing and most used assays for species discrimination, with a critical appraisal of the limitations of each technique. The consensus taxonomy for the genus is outlined, including debatable species designations. Finally, a numerical literature analysis is presented that describes which methods are most used in various countries and regions in the world, and for which purposes.

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Geographical distribution and epidemiological features of Old WorldLeishmania infantumandLeishmania donovanifoci, based on the isoenzyme analysis of 2277 strains

Cited by 43 publications

References 44 publications

Evaluation of four molecular methods to detect Leishmania infection in dogs

Evaluation of four molecular methods to detect Leishmania infection in dogs

Leishmaniases in Greece

Species Typing in Dermal Leishmaniasis

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