Geographically driven adaptation of chilli veinal mottle virus revealed by genetic diversity analysis of the coat protein gene

Gao, Fangluan; Jin, Jing; Zou, Wenchao; Liao, Furong; Shen, Jianguo

doi:10.1007/s00705-016-2761-7

Cited by 25 publications

(23 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here, phylogenetic analysis revealed that MpnDV haplotypes clustered according to geographical origin, except for those found in Liaoning and Shanxi provinces, likely due to long-distance migration of M. persicae nicotianae (Loxdale et al, 1993). Aphids from Liaoning may have crossed the Bohai Sea, subsequently interacting with other populations, while others might have migrated from Shanxi to Yunnan (Gao et al, 2016). From the distribution of haplotypes in the NJ tree and their locations, no significant relationship between haplotype and geographical distance was evident.…”

Section: Discussionmentioning

confidence: 74%

Genetic variability of Myzus persicae nicotianae densovirus based on partial NS and VP gene sequences

Song

Tang

Tang³

et al. 2016

Genet. Mol. Res.

View full text Add to dashboard Cite

We previously described a novel densovirus [Myzus persicae nicotianae densovirus (MpnDV)] infecting M. persicae nicotianae (Hemiptera: Aphididae) with 34% prevalence. This single-stranded DNA virus has a 5480-nucleotide ambisense genome and belongs to the Densovirinae subfamily within the family Parvoviridae. In the present study, we estimated the genetic diversity of MpnDV using partial nonstructural protein (NS) and capsid protein (VP) gene sequences from 10 locations in China. First, we identified MpnDV-positive samples by amplifying a 445-bp fragment with primers MpDVF/MpDVR. Subsequently, we amplified and sequenced COI genes with primers MpCOIF/ MpCOIR, and partial NS and VP sequences with primers MpnDVF1/MpnDVR1. The respective 655-, 1461-, and 423-bp COI, NS, and VP fragments were used to analyze the genetic diversity of MpnDV using MEGA 6.0 and DnaSP 5.0. The high level of identity shared by all COI sequences (>99%) suggested that the aphids sampled were of the same species, and indicated population homogeneity across the 10 locations investigated. The nucleotide diversity of MpnDV sequences (0.0020 ± 0.0025) was significantly higher than that of the COI genes (0.0002 ± 0.0005). The pairwise fixation index for MpnDV was 0.832, and the total gene flow was 0.05. Phylogenetic analysis revealed that the MpnDV haplotypes clustered according to geographical location, except for those from the Liaoning and Shanxi provinces. In conclusion, MpnDV demonstrated a low level of gene flow and high genetic diversity, suggesting that it is vertically transmitted, and implying that endosymbiotic viruses could be used as markers in studies of insect population genetics.

show abstract

Section: Discussionmentioning

confidence: 74%

Genetic variability of Myzus persicae nicotianae densovirus based on partial NS and VP gene sequences

Song

Tang

Tang³

et al. 2016

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…ChiVMV infection greatly inhibits plant growth and causes severe symptoms including mottling, distortion, and systemic necrosis ( Chung et al , 2008 ). The ChiVMV genome is a positive-sense, ssRNA of 9.7 kb, excluding the poly(A) tail ( Vijayapalani et al , 2012 ; Gao et al , 2016 ). Potyviral helper component proteinase (HCPro) is a multifunctional protein mainly involved in aphid transmission and suppression of post-transcriptional gene silencing ( Anandalakshmi et al , 1998 ; Kasschau et al , 1998 ).…”

Section: Introductionmentioning

confidence: 99%

Chilli veinal mottle virus HCPro interacts with catalase to facilitate virus infection in Nicotiana tabacum

Yang

Qiu

Huang

et al. 2020

Journal of Experimental Botany

View full text Add to dashboard Cite

Plant symptoms are derived from specific interactions between virus and host components. However, little is known about viral or host factors that participate in the establishment of systemic necrosis. Here, we showed that helper component proteinase (HCPro) encoded by Chilli veinal mottle virus (ChiVMV), could directly interact with catalase 1 (CAT1) and catalase 3 (CAT3) of Nicotiana tabacum (N. tabacum) in cytoplasm to facilitate viral infection. In vitro, the activities of CAT1 and CAT3 were inhibited by the interaction between HCPro and CATs. The C terminus of HCPro was essential for their interaction and also required for the decrease of enzyme activities. Interestingly, the mRNA and protein level of catalases were up regulated in N. tabacum plants in response to ChiVMV infection. N. tabacum plants with HCPro overexpression or CAT1 knockout were more susceptible to ChiVMV infection, which was similar to that in H2O2 pretreated plants, and the overexpression of CAT1 inhibited ChiVMV accumulation. Besides, neither CAT1 nor CAT3 could affect the RNA silencing suppression (RSS) activity of HCPro. Our results confirmed that the interaction between HCPro and catalase promoted the development of plant systemic necrosis. This work demonstrated a novel role of HCPro in virus infection and pathogenicity.

show abstract

“…The open reading frame (ORF) of ChiVMV encodes a large polyprotein, which is processed into ten functional mature proteins [2,3]. ChiVMV mainly infects solanaceous crops and has caused considerable economic losses to pepper (Capsicum annuum L.) production in Asia and Africa [4][5][6][7][8][9][10][11][12]. Moreover, it has been found highly deleterious to production of tobacco (Nicotiana tabacum L.) production since 2011 in China [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Characterization and a RT-RPA assay for rapid detection of Chilli Veinal mottle virus (ChiVMV) in tobacco

Jiao

et al. 2020

Virol J

View full text Add to dashboard Cite

Background: Chilli veinal mottle virus (ChiVMV), which belongs to the genus Potyvirus of the family Potyviridae, mainly infects solanaceous plants and has caused serious economic losses in Asia and Africa. Tobacco plants infected with ChiVMV suffered from punctate necrosis of leaves, leaf deformation, systemic necrosis of leaves and stems, and eventually plant death. However, ChiVMV infection could not usually be identified given the lack of rapid and efficient detection assays in tobacco plants. Therefore, an isolate of tobacco-infecting ChiVMV (ChiVMV-LZ) was obtained, and a novel isothermal amplification and detection technique, reverse transcription-recombinase polymerase amplification (RT-RPA), was established to detect ChiVMV in tobacco plants. Methods: In this study, the full-length genome of ChiVMV-LZ was obtained using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) assays. The genome sequence of ChiVMV-LZ was characterized by sequence alignment and phylogenetic analysis. Then, a RT-RPA assay was established for rapid and sensitive detection of ChiVMV-LZ in tobacco. Additionally, the established RT-RPA assay was compared to the RT-PCR assay in aspect of sensitivity and application in field-collected tobacco samples. Results: ChiVMV-LZ was isolated from diseased tobacco in Luzhou, Sichuan, China. The tobacco plants inoculated with ChiVMV-LZ showed typical symptoms of yellow and round spots on the leaves, and curled and folded leaf margin, similar to those observed on naturally ChiVMV-infected tobacco in the field. The full-length genomic sequence of ChiVMV-LZ was determined to be 9742 nucleotides. Sequence alignment and phylogenetic analysis showed that ChiVMV-LZ was most closely related to ChiVMV-Yp8 isolated from pepper plants in Sichuan province while distantly related to ChiVMV-YN from tobacco in Yunnan province, indicating a possibly geographical differentiation of ChiVMV isolates. Additionally, a RT-RPA assay was established for rapid detection of ChiVMV in tobacco. The RT-RPA has no cross-reaction with other related tobacco viruses and is about 10-fold more sensitive than conventional RT-PCR method. Conclusion: The characterization of ChiVMV-LZ infecting tobacco was determined, and the established RT-RPA assay provides a reliable and effective method for rapid detection of ChiVMV in tobacco.

show abstract

Geographically driven adaptation of chilli veinal mottle virus revealed by genetic diversity analysis of the coat protein gene

Cited by 25 publications

References 24 publications

Genetic variability of Myzus persicae nicotianae densovirus based on partial NS and VP gene sequences

Genetic variability of Myzus persicae nicotianae densovirus based on partial NS and VP gene sequences

Chilli veinal mottle virus HCPro interacts with catalase to facilitate virus infection in Nicotiana tabacum

Characterization and a RT-RPA assay for rapid detection of Chilli Veinal mottle virus (ChiVMV) in tobacco

Contact Info

Product

Resources

About