Germline development from human pluripotent stem cells toward disease modeling of infertility

Hayashi, Yohei; Saitou, Mitinori; Yamanaka, Shinya

doi:10.1016/j.fertnstert.2012.04.037

Cited by 52 publications

(39 citation statements)

References 104 publications

(113 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Perhaps the ultimate approach would involve the differentiation of pluripotent stem cells into primordial germ cells (PGCs) and ultimately into mature oocytes (Daley, 2007;Handel et al, 2014;Hayashi et al, 2012;Mathews et al, 2009). However, the complexity of establishing an in vitro system for producing functional haploid human oocytes currently limits this application (Irie et al, 2015;Sasaki et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Functional Human Oocytes Generated by Transfer of Polar Body Genomes

O’Neil

Gutiérrez

et al. 2017

Cell Stem Cell

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Functional Human Oocytes Generated by Transfer of Polar Body Genomes

O’Neil

Gutiérrez

et al. 2017

Cell Stem Cell

View full text Add to dashboard Cite

“…Human induced pluripotent stem (hiPS) cells provide a valuable source for generating patientspecific stem cells for regenerative medicine and disease modeling [1,2]. hiPS cells can be generated by reprogramming somatic cells using viral-based methods to deliver various reprogramming factors [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Efficient Generation of Integration-Free Human Induced Pluripotent Stem Cells From Keratinocytes by Simple Transfection of Episomal Vectors

Piao

Hung

Lim

et al. 2014

Stem Cells Translational Medicine

View full text Add to dashboard Cite

Keratinocytes represent an easily accessible cell source for derivation of human induced pluripotent stem (hiPS) cells, reportedly achieving higher reprogramming efficiency than fibroblasts. However, most studies utilized a retroviral or lentiviral method for reprogramming of keratinocytes, which introduces undesirable transgene integrations into the host genome. Moreover, current protocols of generating integration-free hiPS cells from keratinocytes are mostly inefficient. In this paper, we describe a more efficient, simple-to-use, and cost-effective method for generating integrationfree hiPS cells from keratinocytes. Our improved method using lipid-mediated transfection achieved a reprogramming efficiency of ∼0.14% on average. Keratinocyte-derived hiPS cells showed no integration of episomal vectors, expressed stem cell-specific markers and possessed potentials to differentiate into all three germ layers by in vitro embryoid body formation as well as in vivo teratoma formation. To our knowledge, this represents the most efficient method to generate integrationfree hiPS cells from keratinocytes. STEM CELLS TRANSLATIONAL MEDICINE 2014;3:787-791

show abstract

“…First, patient-specific iPSC-derived gametes will permit the characterization of germline disease at the cellular and molecular levels (10). Second, fully functional autologous gametes of iPSC origin might substitute for lost or impaired germ cell function, for example, in the case of cancer survivors.…”

Section: Translational Possibilitiesmentioning

confidence: 99%