GhAAO2 was observed responding to NaHCO3 stress in cotton compared to AAO family genes

Liu, Xiaoyu; Cui, Yupeng; Kang, Ruiqin; Zhang, Hong; Huang, Hui; Lei, Yuqian; Fan, Yapeng; Zhang, Yuexin; Wang, Jing; Xu, Nan; Han, Mingge; Feng, Xixian; Ni, Kesong; Jiang, Tiantian; Rui, Cun; Sun, Liangqing; Chen, Xiugui; Lu, Xuke; Wang, Delong; Wang, Junjuan; Wang, Shuai; Zhao, Lingxia; Guo, Lixue; Chen, Chao; Chen, Quanjia; Ye, Wuwei

doi:10.1186/s12870-022-03999-7

Cited by 3 publications

(3 citation statements)

References 63 publications

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“…For determination of proline (Pro) and malondialdehyde (MDA) contents, the leaves of control and NaCl-treated cotton seedlings were collected at each time point and immediately frozen in liquid nitrogen and stored at −80°C to determine the content of Pro and MDA. The content of Pro was determined using the test kit from Nanjing Jiancheng Bioengineering Institute, the content of MDA was determined using the test kit from Beijing Solarbio Science & Technology Co., Ltd. ( Liu et al., 2022 ; Meng et al., 2023 ).…”

Section: Experimental Materials and Methodsmentioning

confidence: 99%

Raffinose degradation-related gene GhAGAL3 was screened out responding to salinity stress through expression patterns of GhAGALs family genes

Chen,

Cui,

et al. 2023

Front. Plant Sci.

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A-galactosidases (AGALs), the oligosaccharide (RFO) catabolic genes of the raffinose family, play crucial roles in plant growth and development and in adversity stress. They can break down the non-reducing terminal galactose residues of glycolipids and sugar chains. In this study, the whole genome of AGALs was analyzed. Bioinformatics analysis was conducted to analyze members of the AGAL family in Gossypium hirsutum, Gossypium arboreum, Gossypium barbadense, and Gossypium raimondii. Meanwhile, RT-qPCR was carried out to analyze the expression patterns of AGAL family members in different tissues of terrestrial cotton. It was found that a series of environmental factors stimulated the expression of the GhAGAL3 gene. The function of GhAGAL3 was verified through virus-induced gene silencing (VIGS). As a result, GhAGAL3 gene silencing resulted in milder wilting of seedlings than the controls, and a significant increase in the raffinose content in cotton, indicating that GhAGAL3 responded to NaCl stress. The increase in raffinose content improved the tolerance of cotton. Findings in this study lay an important foundation for further research on the role of the GhAGAL3 gene family in the molecular mechanism of abiotic stress resistance in cotton.

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Section: Experimental Materials and Methodsmentioning

confidence: 99%

Raffinose degradation-related gene GhAGAL3 was screened out responding to salinity stress through expression patterns of GhAGALs family genes

Chen,

Cui,

et al. 2023

Front. Plant Sci.

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“…In recent years, studies on ABA have shown that there may be two biosynthetic pathways for ABA in higher plants [5]: (1) the direct pathway is the polymerization of three isopentane units to form the C15 precursor farnesyl pyrophosphate (FPP), which is formed directly from 15-carbon ABA by epoxidation and oxidation of FPP [6]; (2) in the indirect pathway, ABA is synthesized using carotenoids as precursors [7]. Zeaxanthin epoxidase (ZEP) catalyzes the formation of zeaxanthin from cyclic zeaxanthin, which is cleaved to flavin aldehyde by 9-cis-epoxycarotenoid dioxygenase (NCED).…”

Section: Introductionmentioning

confidence: 99%

“…As a key gene in the last step of the ABA synthesis pathway, the biological function of AAO genes has been gradually studied in Arabidopisis [10], cotton [5], rice [8], maize [11], pea [12], and so on. AtAAO4, A. thaliana genes, played a critical role in delaying senescence in siliques by catalyzing aldehyde detoxification [13].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Identification of Drought-Responsive ABA-Aldehyde Oxidase (AAO) Genes in Potato (Solanum tuberosum L.)

Yao,

Zhang,

Zhang

et al. 2023

Plants

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Abscisic acid (ABA) is an important stress hormone that affects plants’ tolerance to stress. Changes in the content of abscisic can have an impact on plant responses to abiotic stress. The abscisic acid aldehyde oxidase (AAO) plays a crucial role in the final step in the synthesis of abscisic acid; therefore, understanding the function of the AAO gene family is of great significance for insight into plants’ response to abiotic stresses. In this study, Solanum tuberosum AAO (StAAO) members were exhaustively explored using genome databases, and nine StAAOs were identified. Chromosomal location analysis indicated that StAAO genes mapped to 4 of the 14 potato chromosomes. Further analyses of gene structure and motif composition showed that members of the specific StAAO subfamily showed relatively conserved characteristics. Phylogenetic relationship analysis indicated that StAAOs proteins were divided into three major clades. Promoter analysis showed that most StAAO promoters contained cis-elements related to abiotic stress response and plant hormones. The results of tissue-specific expression analysis indicated that StAAO4 was predominantly expressed in the roots. Analysis of transcriptome data revealed that StAAO2/4/6 genes responded significantly to drought treatments. Moreover, further qRT-PCR analysis results indicated that StAAO2/4/6 not only significantly responded to drought stress but also to various phytohormone (ABA, SA, and MeJA) and abiotic stresses (salt and low temperature), albeit with different expression patterns. In summary, our study provides comprehensive insights into the sequence characteristics, structural properties, evolutionary relationships, and expression patterns of the StAAO gene family. These findings lay the foundation for a deeper understanding of the StAAO gene family and offer a potential genetic resource for breeding drought-resistant potato varieties.

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Exploring the Role of CottonCMFGenes in Salt Stress Tolerance: Insights from Phylogenetic, Expression, and Functional Analyses

Cui,

Shi,

Wang

et al. 2024

Preprint

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Salt stress significantly limits cotton growth and development, making the improvement of salt tolerance critical. CMF genes, containing a single CCT domain, are promising targets for breeding programs. We identified 101 CMF genes from four cotton species. Phylogenetic analysis grouped these genes into six clusters closely related to Arabidopsis. Gene structure and motif analysis showed high conservation, while collinear analysis indicated segmental duplication as a key factor in CMF gene family expansion. Promoter analysis predicted involvement in light, abiotic stress, plant hormone responses, and growth. Expression analysis revealed differential expression of GhCMF genes across tissues, with some induced by abiotic stress. Co-expression network analysis indicated GhCMF14 interacts with other proteins to resist abiotic stress. Subcellular localization showed GhCMF14 in the nucleus. Silencing GhCMF14 enhanced salt tolerance, with higher chlorophyll and proline content, and lower MDA and H2O2 levels. This study provides insights into CMF gene regulation and their role in cotton salt stress tolerance.

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GhAAO2 was observed responding to NaHCO3 stress in cotton compared to AAO family genes

Cited by 3 publications

References 63 publications

Raffinose degradation-related gene GhAGAL3 was screened out responding to salinity stress through expression patterns of GhAGALs family genes

Raffinose degradation-related gene GhAGAL3 was screened out responding to salinity stress through expression patterns of GhAGALs family genes

Characterization and Identification of Drought-Responsive ABA-Aldehyde Oxidase (AAO) Genes in Potato (Solanum tuberosum L.)

Exploring the Role of CottonCMFGenes in Salt Stress Tolerance: Insights from Phylogenetic, Expression, and Functional Analyses

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