Ghrelin Administration Increases the Bax/Bcl-2 Gene Expression Ratio in the Heart of Chronic Hypoxic Rats

Aliparasti, Mohammad Reza; Alipour, Mohammad Reza; Almasi, Shohreh; Feizi, Hadi

doi:10.15171/apb.2015.027

Cited by 17 publications

(10 citation statements)

References 27 publications

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“…The Bcl-2 gene inhibits apoptosis in three ways ( 13 – 15 ): First, through its action as an antioxidant; second, through its inhibition of the production of endoplasmic reticulum calcium ions; and finally, through its interaction with other apoptosis factors like Bax. Activated Bcl-2 can promote the migration and proliferation of endothelial cells and inhibit apoptosis ( 16 – 18 ). It is generally believed that the pathogenesis of neonatal RDS is due to the large-area of damage to the alveolar-capillary membrane, which in turn causes atelectasis and pulmonary edema.…”

Section: Discussionmentioning

confidence: 99%

Correlation of expression levels of caspase-3 and Bcl-2 in alveolar lavage fluid in neonatal respiratory distress syndrome and prognosis

Lin

Wang

2018

Exp Ther Med

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This study was designed to investigate the correlation between expression levels of cysteine aspartic protease-3 (caspase-3) and B-cell lymphoma gene-2 (Bcl-2) proteins in alveolar lavage fluid and the prognosis of infants with neonatal respiratory distress syndrome (RDS). A total of 150 infants with neonatal RDS undergoing alveolar lavages were divided into four groups: RDS1 (group A, n=42), RDS2 (group B, n=38), RDS3 (group C, n=38) and RDS4 (group D, n=32) according to their thoracic X-ray film grading. The oxygen uptake score, oxygenation saturation, mean airway pressure and expression levels of caspase-3 and Bcl-2 in alveolar lavage fluid of the infants in the four groups were measured and compared. Our results showed higher grading by thoracic X-rays in patients with increased oxygen uptake score, oxygenation index, mean airway pressure, caspase-3 expression level, hospital stay, complications and death rates in all groups; however, the expression levels of Bcl-2 were decreased in those cases, and the differences had statistical significance among the four groups (P<0.05). Analyses for correlation showed a caspase-3 positive area that was positively correlated with oxygen uptake score, oxygenation index and mean airway pressure (P<0.05); and a Bcl-2 expression level that was negatively correlated with oxygen uptake score, oxygenation index and mean airway pressure (P<0.05). Based on our findings, the severity of neonatal RDS is positively correlated with the concentration of caspase-3 in alveolar lavage fluid, and negatively correlated with the expression level of Bcl-2.

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Section: Discussionmentioning

confidence: 99%

Correlation of expression levels of caspase-3 and Bcl-2 in alveolar lavage fluid in neonatal respiratory distress syndrome and prognosis

Lin

Wang

2018

Exp Ther Med

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“…These parameters reflected the cellular condition and iron status assessed in the states of different iron availability in culture medium of H9C2 and L6 lines. These parameters were looking at the expression levels of various genes in the following processes: i) Apoptosis, including B-cell lymphoma (Bcl)-2-associated X protein (Bax; inductor) (33), Bcl-2 (inhibitor) (34) and the Bax/Bcl-2 gene expression ratio (35); ii) proteolysis, including Atrogin-1, which is a marker of protein degradation in myocytes and a marker of muscle atrophy (36); iii) glycolysis, including pyruvate kinase (PKM2; marker of non-oxidative metabolism) (37); iv) intracellular iron metabolism, including transferrin receptor type 1 (TfR1; cellular iron importer) (38), ferroportin (FPN1; cellular iron exporter) (39), FTH heavy chain (iron storage protein) (40) and hepcidin (HAMP; iron metabolism regulator protein) (41).…”

Section: Modulation Of Iron Concentration In the Cellular Environmentmentioning

confidence: 99%

Influence of the availability of iron during hypoxia on the genes associated with apoptotic activity and local iron metabolism in rat H9C2 cardiomyocytes and L6G8C5 skeletal myocytes

Dzięgała

Kasztura

Kobak

et al. 2016

Molecular Medicine Reports

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The differential availability of iron during hypoxia is presumed to affect the functioning of cardiac and skeletal myocytes. Rat H9C2 cardiomyocytes and L6G8C5 myocytes were cultured for 48 h in normoxic or hypoxic conditions at the optimal, reduced or increased iron concentration. The mRNA expression levels of markers of apoptosis [B‑cell lymphoma‑2 (Bcl2; inhibition) and Bcl‑2‑activated X protein (Bax; induction)], atrophy (Atrogin), glycolysis (pyruvate kinase 2; PKM2) and iron metabolism [transferrin receptor 1 (TfR1; iron importer), ferroportin 1 (FPN1; iron exporter), ferritin heavy chain (FTH; iron storage protein) and hepcidin (HAMP; iron regulator)] were determined using reverse transcription‑quantitative polymerase chain reaction, and cell viability was measured using an tetrazolium reduction assay. Cardiomyocytes and myocytes, when exposed to hypoxia, demonstrated an increased Bax/Bcl‑2 gene expression ratio (P<0.05). Additional deferoxamine (DFO) treatment resulted in further increases in Bax/Bcl‑2 in each cell type (P<0.001 each) and this was associated with the 15% loss in viability. The analogous alterations were observed in both cell types upon ammonium ferric citrate (AFC) treatment during hypoxia; however, the increased Bax/Bcl‑2 ratio and associated viability loss was lower compared with that in case of DFO treatment (P<0.05 each). Under hypoxic conditions, myocytes demonstrated an increased expression of PKM2 (P<0.01). Additional DFO treatment caused an increase in the mRNA expression levels of PKM2 and Atrogin‑1 (P<0.001 and P<0.05, respectively), whereas AFC treatment caused an increased mRNA expression of PKM2 (P<0.01) and accompanied decreased mRNA expression of Atrogin‑1 (P<0.05). The expression augmentation of PKM2 during hypoxia was greater upon low iron compared with that of ferric salt treatment (P<0.01). Both cell types upon DFO during hypoxia demonstrated the increased expression of TfR1 and HAMP (all P<0.05), which was associated with the increased Bax/Bcl‑2 ratio (all R>0.6 and P<0.05). In conclusion, during hypoxia iron deficiency impairs the viability of cardiomyocytes and myocytes more severely compared with iron excess. In myocytes, during hypoxia iron may act in a protective manner, since the level of atrophy is decreased in the iron‑salt‑treated cells.

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“…Aliparasti et al apoptosis mechanisms of Wistar rats were investigated and showed an increase of the Bax/Bcl-2 ratio in the hypoxic condition [42]. Similar research carried out by Ciora et al demonstrated the possibility of using probiotics as radioprotective agents in man was raised [43].…”

Section: Discussionmentioning

confidence: 82%

The Effect of Lactobacillus Casei and Lactobacillus Acidophilus Probiotic Isolates on the Expression of BCL2, BAX and CASPASE3 Genes in Wistar Breeding Rat Albindor to evaluate Gamma Irradiation Induced Apoptosis

Changizi

Azadbakht²,

Khanzadeh

et al. 2020

Preprint

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Background: Radiation therapy is one of the most essential components of cancer treatment, so that more than half of cancer patients receive that treatment modality. Radiotherapy of pelvic, spine, or testicles could cause infertility and oligospermia due to direct effect on the gonads. The testicle, as a high radiosensitive tissue, faces functional disorder with the lowest dose of 0.15 Gy and oligospermia with 0.3–0.5 Gy. Since protection against adverse effects of Radiation is essential, studies are being done to find out radioprotectors with the non-toxic properties. Probiotics are living microorganisms, the effects of which on the intestinal microbial flora can boost the immune system.Methods: Male Wistar albino rats were subjected to dietary supplements, including Lactobacillus Casei and Lactobacillus acidophilus, in four groups, for 17 weeks. After being exposed by 2 Gy gamma radiations, the testicular tissue was extracted, and the expression of the apoptotic genes of Bax, Bcl2, and Cspase3 was evaluated.Results: No significant difference was seen in the expression of Bax, and Bcl24-2 genes among the studied groups. However, Caspase3 expression has increased in the group which hasn’t received Radiation but used Lactobacillus Acidophilus.Conclusion: The present results suggested that the use of the aforementioned probiotics mentioned above would reduce the Radiation-induced apoptosis, which then prevented cell death from oligospermia.

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Ghrelin Administration Increases the Bax/Bcl-2 Gene Expression Ratio in the Heart of Chronic Hypoxic Rats

Cited by 17 publications

References 27 publications

Correlation of expression levels of caspase-3 and Bcl-2 in alveolar lavage fluid in neonatal respiratory distress syndrome and prognosis

Correlation of expression levels of caspase-3 and Bcl-2 in alveolar lavage fluid in neonatal respiratory distress syndrome and prognosis

Influence of the availability of iron during hypoxia on the genes associated with apoptotic activity and local iron metabolism in rat H9C2 cardiomyocytes and L6G8C5 skeletal myocytes

The Effect of Lactobacillus Casei and Lactobacillus Acidophilus Probiotic Isolates on the Expression of BCL2, BAX and CASPASE3 Genes in Wistar Breeding Rat Albindor to evaluate Gamma Irradiation Induced Apoptosis

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