Giant Catalytic DNA Particles for Simple and Intuitive Detection of Pb2+

Abstract

DNAzymes have been extensively studied as biosensors because of their unique functionality of cleaving substrate in the presence of metal ion cofactors. However, there are only a few reports on visual detection using gold nanoparticles. Here, we synthesized the DNAzyme microparticle (DzMP) (~1 μm) via rolling circle amplification for detection of Pb2+ without the help of other materials. Then, the substrate strands were labeled with two different fluorophores (6-carboxyfluorescein and Cy5) to visualize the DzMPs and to monitor the separation of substrate strands. Because of their large size, the decline in the number of fluorescent particles in the presence of Pb2+ could be successfully demonstrated by a fluorescence microscopy, presenting a new platform for heavy metal detection.Electronic supplementary materialThe online version of this article (doi:10.1186/s11671-016-1462-1) contains supplementary material, which is available to authorized users.

Erratum to: Giant Catalytic DNA Particles for Simple and Intuitive Detection of Pb2+

“…Unfortunately, the original version of this article [ 1 ] did not contain the following acknowledgement:…”