Gibberellins modulate auxin responses during tomato (<i>Solanum lycopersicum</i> L.) fruit development

Mignolli, Francesco; Vidoz, María Laura; Picciarelli, Piero; Mariotti, Lorenzo

doi:10.1111/ppl.12770

Cited by 21 publications

(10 citation statements)

References 52 publications

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“…We found significantly increased expression levels of genes encoding GA and CK biosynthetic key enzymes, such as GA 20-oxidase 1 (GA20ox1) and cytokinin phosphoribohydrolase 'Lonely guy' (LOG3) ( Table 1). These results were similar to previous reports that upregulation of GA20oxs contributed to active GAs accumulation, which is required for fruit development in tomato [47,48]. The expression of SlLOG2, a CK biosynthetic gene, significantly increased after anthesis [49].…”

Section: Plos Onesupporting

confidence: 91%

Transcriptome sequencing and endogenous phytohormone analysis reveal new insights in CPPU controlling fruit development in kiwifruit (Actinidia chinensis)

Lan

Xiang

et al. 2020

PLoS ONE

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Kiwifruit (Actinidia chinensis) is a rich nutritious fruit crop owing to a markedly higher content of vitamin C and minerals. To promote fruit set and to increase the yield of kiwifruit, forchlorfenuron (CPPU) has been widely applied. However, the molecular details regarding CPPU controlling kiwifruit development, especially at the fastest fruit growth stage, remain unknown. In the present study, we measured the effect of CPPU on developmental regulation in red-fleshed kiwifruit (Actinidia chinensis 'Hongyang'). Additionally, a cytological analysis was performed to clarify the precise changes in the cell structure of the CPPU-treated kiwifruits. Moreover, the concentration of endogenous phytohormones, including indoleacetic acid (IAA), zeatin (ZT), gibberellic acid 3 (GA 3), and abscisic acid (ABA), were measured by Enzyme-linked Immunosorbent Assay (ELISA). Furthermore, RNA-Seq was performed to dissect the complicated molecular mechanisms, with a focus on biosynthesis, metabolism, and signaling compounds, such as endogenous hormones, sugars, and L-ascorbic acid. Our results demonstrated that CPPU treatment not only regulates the size and weight of a single fruit but also improves the quality in 'Hongyang' kiwifruit through the accumulation of both soluble sugar and vitamin C. It was also seen that CPPU regulates kiwifruit development by enhancing cell expansion of epidermal cells and parenchyma cells, while, promoting cell division of subepidermal cells. Additionally, CPPU significantly increased the gibberellin and cytokinin biosynthetic pathway and signaling, while repressing auxin and ABA biosynthetic pathway; thus, signaling plays an essential role in CPPU controlling kiwifruit development. Notably, transcriptomic analysis revealed that a total of 2244 genes, including 352 unannotated genes, were differentially expressed in kiwifruits because of CPPU treatment, including 127 transcription factors. These genes are mainly enriched in plant hormone signal transduction, photosynthesis, MAPK signaling pathway, starch and sucrose metabolism, and phenylpropanoid biosynthesis. Overall, our results highlight that CPPU regulation of kiwifruit development is mainly associated with an antagonistic and/or

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Section: Plos Onesupporting

confidence: 91%

Transcriptome sequencing and endogenous phytohormone analysis reveal new insights in CPPU controlling fruit development in kiwifruit (Actinidia chinensis)

Lan

Xiang

et al. 2020

PLoS ONE

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“…In tomato, gibberellins interact with auxins to drive fruit growth and development 57 . Several members of the auxin signalling pathways (ARFs and Aux/IAA) are involved in the control of cell division and expansion during early fruit development 16,58 .…”

Section: Discussionmentioning

confidence: 99%

The targeted overexpression of SlCDF4 in the fruit enhances tomato size and yield involving gibberellin signalling

Renau‐Morata

Carrillo

Cebolla-Cornejo

et al. 2020

Sci Rep

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Tomato is one of the most widely cultivated vegetable crops and a model for studying fruit biology. Although several genes involved in the traits of fruit quality, development and size have been identified, little is known about the regulatory genes controlling its growth. In this study, we characterized the role of the tomato SlCDF4 gene in fruit development, a cycling DOF-type transcription factor highly expressed in fruits. The targeted overexpression of SlCDF4 gene in the fruit induced an increased yield based on a higher amount of both water and dry matter accumulated in the fruits. Accordingly, transcript levels of genes involved in water transport and cell division and expansion during the fruit enlargement phase also increased. Furthermore, the larger amount of biomass partitioned to the fruit relied on the greater sink strength of the fruits induced by the increased activity of sucrose-metabolising enzymes. Additionally, our results suggest a positive role of SlCDF4 in the gibberellin-signalling pathway through the modulation of GA4 biosynthesis. Finally, the overexpression of SlCDF4 also promoted changes in the profile of carbon and nitrogen compounds related to fruit quality. Overall, our results unveil SlCDF4 as a new key factor controlling tomato size and composition.

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“…Parthenocarpy, another physiological event occurring in the absence of pollination, is characterized by intensive alterations of phytohormones such as auxin, gibberellin (GA), cytokinin or combinations thereof during fruit set ( McAtee et al, 2013 ). In fact, exogenous application of these phytohormones alone can trigger fruit development including fruit set and fruit growth, to a certain extent, and their combinations would induce a normal fruit growth in the absence of fertilization ( Srivastava and Handa, 2005 ; Mignolli et al, 2018 ). Accordingly, increased GA content or perception are associated with parthenocarpic fruits in tomato ( Solanum lycopersicum L.) mutants such as pat ( Mazzucato et al, 1998 ), pat-2 ( Fos et al, 2003 ), pat-3/4 ( Fos et al, 2001 ), whereas facultative parthenocarpic fruits are observed in the procera mutant ( Carrera et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Modifications in Organic Acid Profiles During Fruit Development and Ripening: Correlation or Causation?

et al. 2018

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The pivotal role of phytohormones during fruit development and ripening is considered established knowledge in plant biology. Perhaps less well-known is the growing body of evidence suggesting that organic acids play a key function in plant development and, in particular, in fruit development, maturation and ripening. Here, we critically review the connection between organic acids and the development of both climacteric and non-climacteric fruits. By analyzing the metabolic content of different fruits during their ontogenetic trajectory, we noticed that the content of organic acids in the early stages of fruit development is directly related to the supply of substrates for respiratory processes. Although different organic acid species can be found during fruit development in general, it appears that citrate and malate play major roles in this process, as they accumulate on a broad range of climacteric and non-climacteric fruits. We further highlight the functional significance of changes in organic acid profile in fruits due to either the manipulation of fruit-specific genes or the use of fruit-specific promoters. Despite the complexity behind the fluctuation in organic acid content during fruit development and ripening, we extend our understanding on the importance of organic acids on fruit metabolism and the need to further boost future research. We suggest that engineering organic acid metabolism could improve both qualitative and quantitative traits of crop fruits.

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Gibberellins modulate auxin responses during tomato (Solanum lycopersicum L.) fruit development

Cited by 21 publications

References 52 publications

Transcriptome sequencing and endogenous phytohormone analysis reveal new insights in CPPU controlling fruit development in kiwifruit (Actinidia chinensis)

Transcriptome sequencing and endogenous phytohormone analysis reveal new insights in CPPU controlling fruit development in kiwifruit (Actinidia chinensis)

The targeted overexpression of SlCDF4 in the fruit enhances tomato size and yield involving gibberellin signalling

Modifications in Organic Acid Profiles During Fruit Development and Ripening: Correlation or Causation?

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