Giemsa versus acridine orange staining in the fish micronucleus assay and validation for use in water quality monitoring

In the present study, the acute toxicity of Chromium in fingerlings of Catla catla, an Indian major carp, was evaluated with renewal bioassay method. In vivo studies were designed to assess the extent of Micronucleus Assay, Comet Assay under the exposure of common heavy-metal compounds, namely, Chromium Nitrate, using Catla catla (2n = 20), as a test model. The laboratory acclimatized fishes were divided into four groups. Group I served as positive control and the other three as exposed groups for three different time durations of 7, 14 and 21 days and were subjected to uninterrupted sub lethal concentrations (50% of 96 h LC 50 ). The experiments were planned in such a way that fish from all the groups were sacrificed on the same day. The frequencies of micronuclei and bi-nuclei were evaluated comparatively in peripheral erythrocytes. As a result, it was observed that, the fishes and different tissues showed differential sensitivity to the heavy-metal treatment. A significant increase in the frequencies of micronucleated and binucleated cells and percentage increase in DNA tail (p<0.001) through Alkaline Comet Assay were observed after 21 days of exposure to chromium. Our results also showed decrease in enzyme Superoxide Dismutase (SOD) activity and increase in catalase enzyme due to increasing chromium concentration. The bio-concentration factor profiles of Chromium in Catla catla during sub lethal toxicity study was also calculated.

show abstract

“…This difference can be explained by a lower mitotic rate caused by a lower average temperature and by older fish (Polard et al, 2011).…”

Section: Genotoxicity Of Chromium In Catla Catla By Micronucleus Assaymentioning

confidence: 99%

IN-VIVO EVALUATION OF HEXAVALENT CHROMIUM INDUCED DNA DAMAGE BY ALKALINE COMET ASSAY AND OXIDATIVE STRESS IN CATLA CATLA

Arunachalam

Annamalai

Kuruva

2013

American Journal of Environmental Sciences

View full text Add to dashboard Cite

show abstract

“…Durante la aplicación de los plaguicidas, las lluvias de corta duración durante la época seca facilitan el transporte de residuos a las aguas superficiales (Ferenczi, Ambrus, Wauchope, & Sumner 2002, Polard et al 2011b, Schulz, 2001. En las corrientes de agua dulce con alta contaminación, está descrito el incremento diez veces superior en la concentración de algunos herbicidas y fertilizantes y en la frecuencia de EMN en peces luego de su aplicación en la época seca (Taghavi et al, 2010, Polard et al, 2011a.…”

Section: Fig 2 Frecuencia Media De Emn En Ejemplares Deunclassified

“…Los micronúcleos (MN) se forman durante la división celular como resultado de ruptura cromosómica (clastogenicidad) y/o disfunción del huso mitótico (aneugenicidad) (Al-Sabti & Metcalfe, 1995), por lo que su detección da cuenta de posibles efectos a causa de daño en el ADN. Este biomarcador ha sido ampliamente utilizado en peces porque permite la detección temprana de problemas ambientales en los ecosistemas acuáticos (Al-Sabti & Metcalfe, 1995, Ergene et al, 2007Grisolia & Starling, 2001;Lopez-Barea, 1996;Polard et al, 2011b;Talapatra et al, 2007).…”

unclassified

Evaluación genotóxica del agua del Río Grande (Antioquia, Colombia) mediante frecuencia de eritrocitos micronucleados de Brycon henni (Characiformes: Characidae)

Restrepo

Jiménez

Cardona

et al. 2016

RBT

View full text Add to dashboard Cite

Genotoxic evaluation of Río Grande (Antioquia, Colombia) water using micronucleus frequency in erythrocytes of Brycon henni (Characiformes: Characidae). The permanent monoculture of bananas and plantains farming in the middle of Río Grande (Turbo -Antioquia) requires the application of a variety of pesticides. Inappropriate banana production practices in this region, have often led to waterbody pollution by agrochemicals from leachate and runoff processes. Currently, fish are the most common vertebrates used as bioindicators of water quality, because they are very sensitive to the presence of contaminants. Our main goal with this study was to compare the frequency of micronucleated erythrocytes (MNE) in peripheral blood specimens of fish Brycon henni, from two locations (polluted and unpolluted) in the Rio Grande. We evaluated the frequency of MNE in peripheral blood samples of fish B. henni from each location during two rainy seasons in 2010 and two dry seasons in 2011. Blood samples were collected, fixed for 24 h, and then were stained with Giemsa. Among results, we found that the median frequency of MNE was higher in the polluted site by agrochemical discharges (0.15±0.18), than in the unimpacted site (0.06±0.08). Furthermore, the frequency of MNE in B. henni during the dry season was highly significant for both locations. The results of this study indicated that the analysis of MNE in B. henni could be recommended as a suitable method for in situ detection of environmental genotoxins. Rev. Biol. Trop. 65 (1): 405-414. Epub 2017 March 01.

show abstract

“…In this way, erythrocytes of fish have been shown to be a safe tool for the micronucleus test [4,5]. The micronucleus test is considered an advantageous technique whose analysis is relatively simple.…”

Section: Introductionmentioning

confidence: 99%

Genotoxic Biomarkers in Fishes of the Chapada Das Mesas National Park, Brazil

Stragliotto¹,

Paz²,

Paz³

et al. 2018

National Parks - Management and Conservation

View full text Add to dashboard Cite

Genotoxic and hematological parameters in Hypostomus pusarum and Mylossoma duriventre were used as biomarkers to assess the exposure to environmental stressors within the Chapada das Mesas National Park (PNCM). Fishes were sampled at two sites in PNCM: São Romão and Prata Waterfall. Biometric data (length and weight) were recorded, and blood was collected from all fishes for analysis. The abiotic variables were measured in each region: pH, temperature and dissolved oxygen. A drop of blood from each fish was placed on two microscope slides and smeared. The slides were left to dry at room temperature for 24 h and then fixed in absolute ethanol for 30 min. Means and standard deviations (SDs) of the biometric data of H. pusarum showed length and total weight bigger than M. duriventre. Nuclear morphological changes (NMAs) were identified in the two sampled species for the two collection points. Among the NMAs found, binucleated nucleus (BN), vacuolated nucleus (VC) and micronucleus (MN) were also found in both species; however, in M. duriventre, the frequency of MN and NMA was higher than H. pusarum. The presented data show that methodologies based on biomarkers will be used in the future park management programs.

show abstract

Giemsa versus acridine orange staining in the fish micronucleus assay and validation for use in water quality monitoring

Cited by 31 publications

References 53 publications

<i>IN-VIVO</i> EVALUATION OF HEXAVALENT CHROMIUM INDUCED DNA DAMAGE BY ALKALINE COMET ASSAY AND OXIDATIVE STRESS IN <i>CATLA CATLA</i>

<i>IN-VIVO</i> EVALUATION OF HEXAVALENT CHROMIUM INDUCED DNA DAMAGE BY ALKALINE COMET ASSAY AND OXIDATIVE STRESS IN <i>CATLA CATLA</i>

Evaluación genotóxica del agua del Río Grande (Antioquia, Colombia) mediante frecuencia de eritrocitos micronucleados de Brycon henni (Characiformes: Characidae)

Genotoxic Biomarkers in Fishes of the Chapada Das Mesas National Park, Brazil

Contact Info

Product

Resources

About