Gill Oxidative Stress Protection through the Use of Phytogenics and Galactomannan Oligosaccharides as Functional Additives in Practical Diets for European Sea Bass (Dicentrarchus labrax) Juveniles

Abstract

The aim of the present study is to evaluate the potential of two functional additives as gill endogenous antioxidant capacity boosters in European sea-bass juveniles fed low-FM/FO diets when challenged against physical and biological stressors. For that purpose, two isoenergetic and isonitrogenous diets with low FM (10%) and FO (6%) contents were supplemented with 5000 ppm plant-derived galactomannan–oligosaccharides (GMOS) or 200 ppm of a mixture of garlic and labiate plant essential oils (PHYTO). A control diet was void from supplementation. Fish were fed the experimental diet for nine weeks and subjected to a confinement stress challenge (C challenge) or a confinement stress challenge combined with an exposure to the pathogen Vibrio anguillarum (CI challenge). Both GMOS and PHYTO diets attenuated fish stress response, inducing lower circulating plasma cortisol and down-regulating nfkβ2 and gr relative gene-expression levels in the gill. This attenuated stress response was associated with a minor energetic metabolism response in relation to the down-regulation of nd5 and coxi gene expression.

The Two Genomes of Gilthead Sea Bream (Sparus aurata): a Multi-Omics and Holobiont Approach

“…Phytobiotics in livestock have been often related with the transcriptional modification of several markers of lipid metabolism (Aanyu et al, 2020;Flees et al, 2021;Liu et al, 2022) and mucosal integrity (Firmino et al, 2021a;Serradell et al, 2022;Torrecillas et al, 2021;Wendner et al, 2023). In this work, PHY additive drove a considerable shift of intestinal transcriptional patterns of GS and REF fish, although as indicated before, they mostly uncorrelated with gut microbiota (Fig.…”

Supplementation of Bacillus subtilis and Lactobacillus casei to increase growth performance and immune system of catfish (Clarias gariepinus) due to Aeromonas hydrophila infection

“…The addition of probiotics (either live or dead) to an aquaculture system can have a beneficial impact [17]. The benefits of using probiotics for fish include improving the immune system [18], increasing fish growth and productivity [19,20], improving water quality, reducing ammonia residues and bioremediation agents [21,22], increasing feed efficiency [23], increasing resistance to infectious diseases [5,24], improving the balance of microflora in the fish digestive tract [25,26], and increasing the expression of genes related to immunity, growth, and nutrient metabolism [27].…”