Ginger facilitates cell migration and heat tolerance in mouse fibroblast cells

Abstract

The components of ginger root ( Zingiber officinale Roscoe) are widely used for various medicinal purposes. Several bioactive compounds have been identified in ginger, including 6-, 8- and 10-gingerols, and 6-shogaol, which are agonists of the thermo-sensors transient receptor potential (TRP) cation channel subfamily V member 1 and TRP ankyrin 1. Our previous study demonstrated that ginger powder may affect human metabolism in vivo . However, the effects of the bioactive compounds of ginger on cells have not been completely elucidated. The present study investigated whether ginger powder extracts could modify cell functions in mouse fibroblast cells. The active components of ginger powder extracts were characterized using high-performance liquid chromatography. The activation of protein kinases, actin assembly, cell migration, expression levels of heat shock proteins (HSPs) and cell viability after heat shock were analyzed in NIH3T3 mouse fibroblast cells. Subsequently, 6-, 8-, 10- and 12-gingerols, as well as 6-, 8- and 10-shogaols, were detected in ginger powder extracts. The levels of phosphorylated Akt, mTOR, ERK and p38 MAPK increased after a 10-min stimulation with ginger powder extracts. In addition, HSP expression levels, lamellipodia formation occurring at cell edges, cell migration and tolerance against heat shock were facilitated following ginger powder extract stimulation. These results suggest that ginger modified cell functions, including actin assembly and heat tolerance, in vitro .

Potential effects of gingerol topical application on components of the innate immunity in lactating goat mammary glands

“…Gingerol exhibits anti-inflammatory effects 4 , 5 and heat stimulation-like effects. 24 In a previous study using cultured mouse MECs, a high temperature (41°C) led to a reduction in the amount of claudin-4 and pSTAT3 compared to the normal temperature (37°C), 44 which is consistent with the results of the present study. The lactating MECs during inflammation due to lipopolysaccharide treatment also show increased pSTAT3 and claudin-4 levels.…”

“… 23 In addition, ginger powder extracts containing gingerol regulate cell migration and heat shock proteins in mouse fibroblasts. 24 Furthermore, ginger extract facilitates heat tolerance in mouse fibroblast cells and broilers. 24 , 25 These observations indicate that gingerol has the potential to induce heat treatment-like effects.…”

“… 24 Furthermore, ginger extract facilitates heat tolerance in mouse fibroblast cells and broilers. 24 , 25 These observations indicate that gingerol has the potential to induce heat treatment-like effects. However, oral administration of polyphenols often causes a decrease in biological activities in target organs through conjugation and metabolic conversion.…”