Ginkgolide B Suppresses Intercellular Adhesion Molecule-1 Expression via Blocking Nuclear Factor-κB Activation in Human Vascular Endothelial Cells Stimulated by Oxidized Low-Density Lipoprotein

Abstract

Abstract. Atherosclerosis is a complex inflammatory arterial disease. Oxidized low-density lipoprotein (ox-LDL) is directly associated with chronic vascular inflammation. In the current study, we tested the hypothesis that ginkgolide B, a component of traditional Chinese herbal medicine for heart disorder, may affect ox-LDL-induced inflammatory responses in human umbilical vein endothelial cells (HUVECs). The results showed that the ox-LDL treatment caused a significantly increase in the expression of intercellular adhesion molecule-1 (ICAM-1) in HUVECs, which was associated with a dramatic augmentation in phosphorylation of IκB and relocation of nuclear factor-κB (NF-κB) into the nuclei. Interestingly, the ox-LDL-induced ICAM-1 expression and NF-κB relocation could be attenuated by addition of ginkgolide B. Moreover, ginkgolide B significantly reduces ox-LDL-induced generation of reactive oxygen species (ROS). In conclusion, ginkgolide B may decrease inflammatory responses induced by ox-LDL via blocking NF-κB signaling and inhibiting ROS generation in HUVECs.

Ginkgolide B inhibits renal cyst development in in vitro and in vivo cyst models

“…Ginkgolide B, the major bioactive component of G. biloba extracts, has been reported to play multiple pharmacological roles as mentioned above (11,14,19,21,38,40). Recent studies have found that ginkgolide B may have anticancer properties in molecular, cellular, and whole animal models.…”

Inhibitory effects of casticin on migration of eosinophil and expression of chemokines and adhesion molecules in A549 lung epithelial cells via NF-κB inactivation

“…These findings suggest that the inhibitory effects of casticin on the release of eotaxin, RANTES, VCAM-1, and ICAM-1 in lung epithelial cells and subsequent eotaxin chemotaxis are associated with the suppression of the NF-B signaling pathway. The expression of eotaxin, RANTES, VCAM-1, ICAM-1 and activation of eosinophilic inflammation involved in the pathogenesis of asthma is known to be mediated by the NF-B signaling cascade (Wong et al, 2002(Wong et al, , 2005Kudo et al, 2005;Kletsas et al, 2006;Zerfaoui et al, 2008;Li et al, 2009).…”

Paeonol Inhibits Oxidized Low Density Lipoprotein-Induced Monocyte Adhesion to Vascular Endothelial Cells by Inhibiting the Mitogen Activated Protein Kinase Pathway

“…Oxidized Low-Density Lipoprotein (ox-LDL) plays an important role in the progression of atherosclerosis. 2) Ox-LDL can reduce endothelial regeneration 3) and activate endothelial adhesive properties. In addition, ox-LDL initiates monocyte invasion in subendothelial space.…”