Ginsenoside Rb 1: A novel therapeutic agent in Staphylococcus aureus-induced Acute Lung Injury with special reference to Oxidative stress and Apoptosis

Shaukat, Aftab; Yang, Chao; Yang, Ye; Guo, Yanxia; Jiang, Kangfeng; Guo, Shanchun; Liu, Junfeng; Zhang, Tao; Zhao, Gan; Ma, Xiaofei; Wu, Zhiming; Zhou, Qingqing; Akhtar, Muhammad Faheem; Zahoor, Arshad; Umar, Talha; Shaukat, Irfan; Hanif, Sana; Rajput, Shahid Ali; Hassan, Mubashar; Mehmood, Khalid; Zhang, Hua; Wang, Xiaoyan; Yin, Nannan; Deng, Ganzhen

doi:10.1016/j.micpath.2020.104109

Cited by 17 publications

(11 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S. aureus activates Toll-like receptor 2-NF-Kb /MAPK signalling and apoptosis. 50,51 CpG-ODN promotes S. aureus-stimulated phagocytosis and autophagy through JNK/P38 signal pathway in macrophage. 12 In our study, MG pretreatment only obviously inhibited JNK signal pathway, which protected RAW264.7 cells from apoptosis and autophagy induced by S. aureus.…”

Section: Discussionmentioning

confidence: 99%

Mangiferin Inhibits Apoptosis and Autophagy Induced by Staphylococcus aureus in RAW264.7 Cells

Xu¹,

Yao²,

Wang³

et al. 2020

JIR

View full text Add to dashboard Cite

Purpose Staphylococcus aureus ( S. aureus ) is an important bacterial pathogen, which creates infective inflammation to human being and animals. Mangiferin (MG) is one of the natural flavonoids with anti-inflammatory, anti-bacterial, and anti-oxidative properties. However, the anti-apoptosis and anti-autophagy of MG are unknown. Hence, this study was aimed to research the inhibition of MG on S. aureus -induced apoptosis and autophagy in RAW264.7 cells. Methods The RAW264.7 cells were pretreated with MG, or pretreated with SP600125 or anisomycin synchronously, and then infected with S. aureus (MOI=100:1). The viability and proliferation status of RAW264.7 cells were detected by MTT and EdU assay. The relative expression of TNF-α, IL-6 and IL-10 protein was tested with ELISA. The levels of Bax, Bcl-2, caspase-3, c-Jun N-terminal kinase (JNK), extracellular-regulated protein kinase (ERK), p38, LC3, Beclin-1, p62, phosphorylated JNK, phosphorylated p38 and phosphorylated ERK in cells were detected by Western blotting. The apoptosis rate of RAW264.7 cells was analyzed by flow cytometric assay. Results The study showed that MG significantly attenuated RAW264.7 cells apoptosis and autophagy caused by S. aureus . MG alleviated S. aureus -induced apoptosis by down-regulating the protein level of active caspase-3 and Bax and up-regulating the level of Bcl-2. MG also inhibited S. aureus -induced autophagy via decreasing the protein level of LC3-II/LC3-I and Beclin-1 or increasing the protein expression of p62. This protective role was dependent on the up-regulation of JNK signal pathway, which was confirmed by using JNK agonist and inhibitor. Conclusion Our results demonstrated that MG might protect RAW264.7 cells from S. aureus -induced apoptosis and autophagy via inhibiting JNK/Bax-dependent signal pathway. Therefore, MG may be a potential agent against pathological cell damage induced by S. aureus infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Mangiferin Inhibits Apoptosis and Autophagy Induced by Staphylococcus aureus in RAW264.7 Cells

Xu¹,

Yao²,

Wang³

et al. 2020

JIR

View full text Add to dashboard Cite

show abstract

“…Normally, Nrf2 is located in bound form in the cytosol with reduced kelch‐like ECH‐associated protein 1 (Keap1). Dissociated Nrf2 translocates to the nucleus, where it triggers the transcription of phase II or other adaptive response genes, including enzymes involved in GSH metabolism, NQO1,2, and HO‐1 226,550,553,554,558,561–577 …”

Section: Core Rational Of the Adaptogenic Conceptmentioning

confidence: 99%

“…Dissociated Nrf2 translocates to the nucleus, where it triggers the transcription of phase II or other adaptive response genes, including enzymes involved in GSH metabolism, NQO1,2, and HO-1. 226,550,553,554,558,[561][562][563][564][565][566][567][568][569][570][571][572][573][574][575][576][577] The efficacy of adaptogens in the treatment of acute respiratory tract diseases is possibly also partially associated with the downregulation of proinflammatory NF-kB signaling in various cells and tissues involved in the acute inflammatory response.…”

Section: Aging-associated Disordersmentioning

confidence: 99%

Evolution of the adaptogenic concept from traditional use to medical systems: Pharmacology of stress‐ and aging‐related diseases

Panossian¹,

Efferth

Shikov

et al. 2020

Medicinal Research Reviews

209

124

View full text Add to dashboard Cite

Adaptogens comprise a category of herbal medicinal and nutritional products promoting adaptability, resilience, and survival of living organisms in stress. The aim of this review was to summarize the growing knowledge about common adaptogenic plants used in various traditional medical systems (TMS) and conventional medicine and to provide a modern rationale for their use in the treatment of stress‐induced and aging‐related disorders. Adaptogens have pharmacologically pleiotropic effects on the neuroendocrine‐immune system, which explain their traditional use for the treatment of a wide range of conditions. They exhibit a biphasic dose‐effect response: at low doses they function as mild stress‐mimetics, which activate the adaptive stress‐response signaling pathways to cope with severe stress. That is in line with their traditional use for preventing premature aging and to maintain good health and vitality. However, the potential of adaptogens remains poorly explored. Treatment of stress and aging‐related diseases require novel approaches. Some combinations of adaptogenic plants provide unique effects due to their synergistic interactions in organisms not obtainable by any ingredient independently. Further progress in this field needs to focus on discovering new combinations of adaptogens based on traditional medical concepts. Robust and rigorous approaches including network pharmacology and systems pharmacology could help in analyzing potential synergistic effects and, more broadly, future uses of adaptogens. In conclusion, the evolution of the adaptogenic concept has led back to basics of TMS and a new level of understanding of holistic approach. It provides a rationale for their use in stress‐induced and aging‐related diseases.

show abstract

“…GnRb1 is a glycoside from the class of triterpenoid saponins, found in Panax ginseng, that has a long history of being used in China to cure various ailments. Its main biopharmacological properties includes cardioprotective [ 26 , 27 ], neuroprotective [ 28 , 29 ], nephroprotective [ 30 ], immunoregulatory [ 31 ], and anti-inflammatory properties [ 15 , 32 , 33 ]. GnRb1 was proved to suppress LPS-induced ALI [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…The characteristic pathological features of LPS-stimulated endometritis include infiltration of neutrophils, secretion of chemokines and cytokines, and edema [ 34 ]. MPO activity is a biomarker of the accumulation of neutrophils during acute inflammation caused by S. aureus [ 32 , 33 ] and E. coli [ 5 , 8 ]. The results indicated that MPO activity was increased in the LPS group.…”

Section: Discussionmentioning

confidence: 99%

Ginsenoside Rb1 Mitigates Escherichia coli Lipopolysaccharide-Induced Endometritis through TLR4-Mediated NF-κB Pathway

Shaukat

Rajput

et al. 2021

Molecules

Self Cite

View full text Add to dashboard Cite

Endometritis is the inflammatory response of the endometrial lining of the uterus and is associated with low conception rates, early embryonic mortality, and prolonged inter-calving intervals, and thus poses huge economic losses to the dairy industry worldwide. Ginsenoside Rb1 (GnRb1) is a natural compound obtained from the roots of Panax ginseng, having several pharmacological and biological properties. However, the anti-inflammatory properties of GnRb1 in lipopolysaccharide (LPS)-challenged endometritis through the TLR4-mediated NF-κB signaling pathway has not yet been researched. This study was planned to evaluate the mechanisms of how GnRb1 rescues LPS-induced endometritis. In the present research, histopathological findings revealed that GnRb1 ameliorated LPS-triggered uterine injury. The ELISA and RT-qPCR assay findings indicated that GnRb1 suppressed the expression level of pro-inflammatory molecules (TNF-α, IL-1β and IL-6) and boosted the level of anti-inflammatory (IL-10) cytokine. Furthermore, the molecular study suggested that GnRb1 attenuated TLR4-mediated NF-κB signaling. The results demonstrated the therapeutic efficacy of GnRb1 in the mouse model of LPS-triggered endometritis via the inhibition of the TLR4-associated NF-κB pathway. Taken together, this study provides a baseline for the protective effect of GnRb1 to treat endometritis in both humans and animals.

show abstract

Ginsenoside Rb 1: A novel therapeutic agent in Staphylococcus aureus-induced Acute Lung Injury with special reference to Oxidative stress and Apoptosis

Cited by 17 publications

References 60 publications

<p>Mangiferin Inhibits Apoptosis and Autophagy Induced by <em>Staphylococcus aureus</em> in RAW264.7 Cells</p>

<p>Mangiferin Inhibits Apoptosis and Autophagy Induced by <em>Staphylococcus aureus</em> in RAW264.7 Cells</p>

Evolution of the adaptogenic concept from traditional use to medical systems: Pharmacology of stress‐ and aging‐related diseases

Ginsenoside Rb1 Mitigates Escherichia coli Lipopolysaccharide-Induced Endometritis through TLR4-Mediated NF-κB Pathway

Contact Info

Product

Resources

About