Ginsenoside-Rd, a purified component from panax notoginseng saponins, prevents atherosclerosis in apoE knockout mice

Li, Jie; Xie, Zhizhong; Tang, Yong-Bo; Zhou, Jia‐Guo; Guan, Yalin

doi:10.1016/j.ejphar.2010.11.017

Cited by 71 publications

(36 citation statements)

References 32 publications

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“…P. notoginseng saponins attenuate atherogenesis in rabbits [104] and radix notoginseng has hypolipidemic and anti-oxidant activities in rats fed a high fat diet [105]. Ginsenoside-Rd prevents atherosclerosis in ApoE-knockout mice [106]. Overall, these results suggest that P. ginseng improves the lipid profile.…”

Section: Efficacy Of Adjusting Blood Lipid Profilementioning

confidence: 84%

Cardiovascular Diseases and Panax ginseng: A Review on Molecular Mechanisms and Medical Applications

Kim

2012

Journal of Ginseng Research

213

142

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Section: Efficacy Of Adjusting Blood Lipid Profilementioning

confidence: 84%

Cardiovascular Diseases and Panax ginseng: A Review on Molecular Mechanisms and Medical Applications

Kim

2012

Journal of Ginseng Research

213

142

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“…Pharmacological studies of LJT have shown antiallergic [2,3], antitumor, immunomodulatory [4], acute toxicity [5], and gastroprotective [6] effects. Recently, the single herbs of LJT, including Ginseng Radix Alba [7], Zingiberis Rhizoma [8], and Glycyrrhizae Radix et Rhizoma [9] were reported to have an inhibitory effects against atherosclerosis. However, studies on the simultaneous analysis and inhibitory effect on low-density lipoprotein (LDL) oxidation by LJT have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification and inhibitory effect on LDL oxidation of the traditional Korean medicine, Leejung-tang

Seo

Kim

et al. 2014

BMC Complement Altern Med

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BackgroundLeejung-tang (LJT) is a traditional Korean herbal medicine for the treatment of gastrointestinal disorders. In this study, we performed quantification analysis of five marker components, liquiritin (1), ginsenoside Rg1 (2), ginsenoside Rb1 (3), glycyrrhizin (4), and 6-gingerol (5) in LJT using a high performance liquid chromatography-photodiode array (HPLC–PDA). In addition, we investigated the inhibitory effect on low-density lipoprotein (LDL) oxidation by the LJT sample.MethodsCompounds 1–5 were separated within 35 min using a Gemini C18 column. The mobile phase used gradient elution with 1.0% (v/v) aqueous acetic acid (A) and 1.0% (v/v) acetic acid in acetonitrile (B). The flow rate was 1.0 mL/min and the detector was a photodiode array (PDA) set at 203 nm, 254 nm, and 280 nm. The inhibitory effect on LDL oxidation conduct an experiment on thiobarbituric acid reactive substance (TBARS) assay, relative electrophoretic mobility (REM) assay, and electrophoresis of ApoB fragmentation of LJT.ResultsCalibration curves of compounds 1–5 showed good linearity (r2 ≥0.9995) in different concentration ranges. The recoveries of compounds 1–5 were in the range of 98.90–103.39%, with relative standard deviations (RSD) below 3.0%. The RSDs (%) of intra-day and inter-day precision were 0.10–1.08% and 0.29–1.87%, respectively. The inhibitory effect of LJT on Cu2+-induced LDL oxidation was defined by TBARS assay (IC50: 165.7 μg/mL) and REM of oxLDL (decrease of 50% at 127.7 μg/mL). Furthermore LJT reduced the fragmentation of ApoB of oxLDL in a dose-dependent manner.ConclusionsThe established HPLC-PDA method will be helpful to improve quality control of LJT. In addition, LJT is a potential LDL oxidation inhibitor.

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“…It has been found that PNS have an anti-inflammatory activity beyond its antioxidant property [14,15,19,20]. The pro-inflammatory factors including VCAM-1, ICAM-1 and MCP-1 are responsible for the accumulation of macrophages into the atheroma-prone area.…”

Section: Pns Treatment Inactivates Nf-κb and Inhibits Expression Of Amentioning

confidence: 99%

Panax Notogingseng Saponins Suppress RAGE/ MAPK Signaling and NF-kappaB Activation in Apolipoprotein-E-deficient Atherosclerosis-prone mice

Dou

Lu²,

Shen³

et al. 2012

Cell Physiol Biochem

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Background: Panax notoginseng saponins (PNS) extracted from the roots of panax notoginseng are free radical-scavenger, with an antioxidant property, capable of inhibiting expression of adhesion molecules and chemokines. This study was designed to test the effects of PNS administration in apolipoprotein (apo)-E-deficient mice on the activation of JNK, p38^MAPK, ERK1/2 and NF-ĸB, and the expression of RAGE, adhesion molecules and chemokines in the atherosclerotic lesions. Methods: Wild type and apoE-null mice (male, 10-week-old) were treated with PNS for 4 weeks. Peripheral blood was collected for assessing the serum levels of glucose, lipids and MDA, and activities of SOD and GSH. The sizes of atherosclerotic lesions and numbers of macrophages in the branchiocephalic arteries, and the reactive oxygen species (ROS) production in the aortic root were analyzed. The levels of CD68, Galectin-3, RAGE, JNK, phosphor-JNK, p38^MAPK, phosphor-p38^MAPK, ERK1/2, phosphor-ERK1/2, I-ĸB, phosphor-I-ĸB (Ser32), NF-ĸB, phosphor-NF-ĸB, MCP-1, VCAM-1 and ICAM-1 in the descending arteries were identified by Western blot. Results: The atherosclerotic lesion sizes, and macrophage numbers, but not the smooth muscle cell amounts and the collagen content, were decreased in apoE^-/mice treated with PNS. After PNS administration for 4 weeks, the apoE^-/-mice displayed reduced level of serum MDA and enhanced activity of SOD and GSH, accompanied by impaired ROS generation in the aortic root. Moreover, PNS down-regulated the expression of VCAM-1, ICAM-1 and MCP-1, accompanied by reduced expression of RAGE and suppressed the activation of NF-ĸB, JNK, p38^MAPK and ERK1/2. Conclusion: PNS may inhibit progression of atherosclerotic lesions via their antioxidant/anti-inflammatory biological properties. PNS suppress the RAGE/MAPK signaling pathways, inactivate NF-ĸB, and reduce expression of pro-inflammatory factors, such as VCAM-1, ICAM-1 and MCP-1 in atherosclerotic lesions of apoE^-/-mice.

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Ginsenoside-Rd, a purified component from panax notoginseng saponins, prevents atherosclerosis in apoE knockout mice

Cited by 71 publications

References 32 publications

Cardiovascular Diseases and Panax ginseng: A Review on Molecular Mechanisms and Medical Applications

Cardiovascular Diseases and Panax ginseng: A Review on Molecular Mechanisms and Medical Applications

Simultaneous quantification and inhibitory effect on LDL oxidation of the traditional Korean medicine, Leejung-tang

Panax Notogingseng Saponins Suppress RAGE/ MAPK Signaling and NF-kappaB Activation in Apolipoprotein-E-deficient Atherosclerosis-prone mice

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