Although the skies over Serbian territory are heavily used by air traffic, and this type of traffic, i.e. airplanes, can have a significant impact on the environment and the health of the population, relatively little attention is paid to this issue both at the level of the Republic of Serbia and at the level of local self-government units. In this work, using Geographic Information Systems, the flights of the national airline Air Serbia over the territory of Serbia in September 2023 were analyzed based on data downloaded from the Flightradar24.com portal. The analysis was carried out at the level of local self-government units in Serbia and for fields of 10 x 10 kilometers size in the territory of the Republic of Serbia. For each local self-government unit and each field, data on the total number of Air Serbia flights over its territory, as well as data on the average flight altitude, flight speed over their territories, total and average flight length over the territory of the local self-government units and the average flight length per unit area of the local self-government units were obtained. The data obtained, together with the data on the flights of other airlines over the territory of Serbia over a long period of time, should represent the basis for the implementation of appropriate environmental protection measures, primarily with the aim of reducing air and noise pollution and the negative impact on human health.