Glial cell line-derived neurotrophic factor improves intrastriatal graft survival of stored dopaminergic cells

TGF-β Family Signaling in Neural and Neuronal Differentiation, Development, and Function

“…TGF-b and Shh cooperatively induce DNs from ventral midbrain floor cells in vitro and in chick embryo in vivo Farkas et al 2003;, whereas Tgfb2 2/ ; Tgfb3 2/2 mice have a severe reduction in DNs at E14.5 (Roussa et al 2006). Glialderived neurotrophic factor (GDNF) promotes survival of embryonic DNs and inhibits the apoptotic death in postnatal midbrain DNs in vitro (Apostolides et al 1998;Burke 2003). Together, TGF-b2 and TGF-b3 synergize with GDNF to facilitate its signaling (Peterziel et al 2002), whereas TGF-b neutralizing antibodies abolish the survival-promoting effects of GDNF in vitro and in vivo (Krieglstein et al 1998;Schober et al 2007).…”

Neurospheres modified to produce glial cell line‐derived neurotrophic factor increase the survival of transplanted dopamine neurons

“…When this is done, GDNF can support the survival and outgrowth of DA neurons following transplantation (Johansson et al, 1995). In addition, GDNF added to cell suspensions of embryonic ventral mesencephalic (VM) tissue improves the survival of DA neurons following grafting into the denervated striatum (Apostolides et al, 1998;Mehta et al, 1998;Sullivan et al, 1998). Other studies have shown that intermittent injections of GDNF in the vicinity of intrastriatal nigral cell suspension grafts have similar effects on improving the survival and/or fibre outgrowth of transplanted DA neurons (Rosenblad et al, 1996;Sinclair et al, 1996;Yurek, 1998).…”

The Potential of Neurotrophic Factors for the Treatment of Parkinson’s Disease

“…Rosenblad and colleagues reported that repeated injections of this factor adjacent to embryonic rat ventral midbrain grafts in the 6-OHDA-lesioned rat striatum improved the survival of the grafted dopaminergic cells and induced earlier recovery of motor function than untreated grafts (Rosenblad et al, 1996). Improvements in the survival of grafted dopaminergic neurones and their integration into the host striatum were also reported after pre-incubation of the grafts with GDNF (Apostolides et al, 1998;Granholm et al, 1997;Yurek, 1998). Injection of GDNF along the nigrostriatal tract stimulated the outgrowth of dopaminergic fibres from intranigral grafts towards the striatum (Sinclair et al, 1996;Tang et al, 1998;Wang, Y. et al, 1996).…”