Glial Cell Line–Derived Neurotrophic Factor Enhances Human Islet Posttransplantation Survival

Mwangi, Simon M.; Usta, Yousef; Shahnavaz, Nikrad; Joseph, Irene; Ávila, José G.; Cano, Jose; Chetty, V. K.; Larsen, Christian; Sitaraman, Shanthi V.; Srinivasan, Shanthi

doi:10.1097/tp.0b013e31822bc95a

Cited by 14 publications

(21 citation statements)

References 18 publications

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“…All studies included in this systematic review used the Ricordi's semi-automated technique for islet isolation. 54 Twenty-three studies (62.2%) used CRML 1066 as the islet culture media after isolation, 16,17,19,24,25,27,[32][33][34][35][37][38][39][41][42][43][47][48][49][50][51][52][53] 4 studies used Miami media, 22,23,29,30 3 used M199 media, 21,31,44 6 of them did not report the media used, 18,26,28,36,40,45 and one study used Ham's/F10 media. 46 All studies had an experimental group where modifications Table 1.…”

Section: Literature Searchmentioning

confidence: 99%

“…1st Author (year) Ref "Antiapoptotic/anti-inflammatory/antioxidant" 23 9 ---Pure: >90; Impure: 40-60 Moriscot (2007) 24 -----Mwangi (2011) 34 37 4 ----Daoud (2010) 38 ---<8 >80 Daoud (2011) 39 ---<8 >80 Kitzmann (2014) 40 ---low purity Maillard (2011) 41 8 2 4 -61 -5.5-9 -Matsushima (2016) 42 -----Marchioli (2015) 43 -----Murray (2009) 44 -----Papas (2005) 45 3 --->90 Zhang (2012) 46 ----»90…”

Section: Groups Of Additives N Of Donorsmentioning

confidence: 99%

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Use of additives, scaffolds and extracellular matrix components for improvement of human pancreatic islet outcomes in vitro: A systematic review

Lemos

Brondani

Dieter

et al. 2017

Islets

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Pancreatic islet transplantation is an established treatment to restore insulin independence in type 1 diabetic patients. Its success rates have increased lately based on improvements in immunosuppressive therapies and on islet isolation and culture. It is known that the quality and quantity of viable transplanted islets are crucial for the achievement of insulin independence and some studies have shown that a significant number of islets are lost during culture time. Thus, in an effort to improve islet yield during culture period, researchers have tested a variety of additives in culture media as well as alternative culture devices, such as scaffolds. However, due to the use of different categories of additives or devices, it is difficult to draw a conclusion on the benefits of these strategies. Therefore, the aim of this systematic review was to summarize the results of studies that described the use of medium additives, scaffolds or extracellular matrix (ECM) components during human pancreatic islets culture. PubMed and Embase repositories were searched. Of 5083 articles retrieved, a total of 37 articles fulfilled the eligibility criteria and were included in the review. After data extraction, articles were grouped as follows: 1) "antiapoptotic/anti-inflammatory/antioxidant," 2) "hormone," 3) "sulphonylureas," 4) "serum supplements," and 5) "scaffolds or ECM components." The effects of the reviewed additives, ECM or scaffolds on islet viability, apoptosis and function (glucose-stimulated insulin secretion - GSIS) were heterogeneous, making any major conclusion hard to sustain. Overall, some "antiapoptotic/anti-inflammatory/antioxidant" additives decreased apoptosis and improved GSIS. Moreover, islet culture with ECM components or scaffolds increased GSIS. More studies are needed to define the real impact of these strategies in improving islet transplantation outcomes.

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Section: Literature Searchmentioning

confidence: 99%

Section: Groups Of Additives N Of Donorsmentioning

confidence: 99%

Use of additives, scaffolds and extracellular matrix components for improvement of human pancreatic islet outcomes in vitro: A systematic review

Lemos

Brondani

Dieter

et al. 2017

Islets

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“…The islets were encapsulated in alginate with disc‐shaped geometry (5 mm × 0.5 mm) to provide optimal diffusion of nutrients and oxygen to encapsulated islets and efficient removal of islet‐derived hormones and metabolic waste products. In addition, islets produce and secrete various neurotrophic factors (Lucini et al, ; Rosenbaum et al, ; Vidaltamayo et al, ) playing an important role in pancreatic beta‐cell survival and function (Mwangi et al, ; Yamanaka et al, ). The exchange of glucose, insulin, neurotrophic factors, oxygen and waste products between encapsulated islets and surrounding tissues and fluids was obviously accelerated by vascularization of a thin layer of fibrotic tissue found around the islet‐containing alginate discs.…”

Section: Discussionmentioning

confidence: 99%

Insulin delivery to the brain using intracranial implantation of alginate-encapsulated pancreatic islets

Bloch

Vanichkin

Gil-Ad

et al. 2017

J Tissue Eng Regen Med

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There is increasing evidence supporting a link between cognitive dysfunctions and impaired brain insulin signalling. Insulin therapy has previously been tested as an approach to ameliorate brain insulin resistance and deficiency in patients with various brain disorders. However, current strategies for insulin delivery to the brain may induce severe hypoglycaemia when injected peripherally or show poor uptake when delivered intranasally. Recently, we have shown that intracranial transplantation of naked pancreatic islets increased insulin content in the brain and attenuated cognitive dysfunctions without altering peripheral glucose homeostasis in rats with schizophrenia-like syndrome. In this study, we show that intracranial implantation of 50 pancreatic islets encapsulated in disc-shaped alginate is sufficient to elevate insulin content in the rat brain. Three weeks after implantation, the islets displayed intact morphology, intensive hormone staining and glucose-sensitive insulin release. The ultrapure alginate with high guluronic acid content used for islet encapsulation demonstrated good biocompatibility and stability after intracranial implantation. All implanted animals were normoglycaemic and normoinsulinaemic. In conclusion, the intracranial implantation of a small amount of alginate-encapsulated islets is an efficient tool for metabolically regulated insulin delivery to the brain. Copyright © 2017 John Wiley & Sons, Ltd.

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“…By the same mechanism, Pefabloc as a serine protease inhibitor, can inhibit serine proteases that affect islets during the isolation procedure (85). Supplementation of human islet culture medium with glial cell line-derived neurotrophic factor (GDNF) has been shown to improve human islet survival and post-transplantation function in diabetic mice (86). In addition, treatment of islets prior to transplantation by heparin and fusion proteins such as soluble TNF-a, which inhibit the inflammatory response, can improve engraftment and islet survival (79,87,88).…”

Section: Improvement Of Pancreas Procurement Islet Isolation and Culmentioning

confidence: 99%

THERAPY OF ENDOCRINE DISEASE: Islet transplantation for type 1 diabetes: so close and yet so far away

Khosravi‐Maharlooei¹,

Hajizadeh‐Saffar²,

Tahamtani³

et al. 2015

European Journal of Endocrinology

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Over the past decades, tremendous efforts have been made to establish pancreatic islet transplantation as a standard therapy for type 1 diabetes. Recent advances in islet transplantation have resulted in steady improvements in the 5-year insulin independence rates for diabetic patients. Here we review the key challenges encountered in the islet transplantation field which include islet source limitation, sub-optimal engraftment of islets, lack of oxygen and blood supply for transplanted islets, and immune rejection of islets. Additionally, we discuss possible solutions for these challenges.

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Glial Cell Line–Derived Neurotrophic Factor Enhances Human Islet Posttransplantation Survival

Cited by 14 publications

References 18 publications

Use of additives, scaffolds and extracellular matrix components for improvement of human pancreatic islet outcomes in vitro: A systematic review

Use of additives, scaffolds and extracellular matrix components for improvement of human pancreatic islet outcomes in vitro: A systematic review

Insulin delivery to the brain using intracranial implantation of alginate-encapsulated pancreatic islets

THERAPY OF ENDOCRINE DISEASE: Islet transplantation for type 1 diabetes: so close and yet so far away

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