Glial fibrillary acidic protein increases in Müller cells after retinal detachment

Erickson, Page A.; Fisher, Steven K.; Guérin, Christopher J.; Anderson, Don H.; Kaska, Deborah D.

doi:10.1016/s0014-4835(87)80023-4

Cited by 164 publications

(98 citation statements)

References 24 publications

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“…49,50 Mü ller cells that do not express GFAP under physiological conditions are known to express GFAP in pathological situations. 51,52 The present results indicate that the injection of LPS provoked a significant alteration in retinal Mü ller cells, as shown by an increase in GFAP immunoreactivity that was prevented by melatonin.…”

Section: Discussionsupporting

confidence: 59%

Therapeutic Effect of Melatonin in Experimental Uveitis

Sande

Fernandez

Marcos

et al. 2008

The American Journal of Pathology

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Uveitis is a common ophthalmic disorder that can be induced in hamsters by a single intravitreal injection of bacterial lipopolysaccharide (LPS). To examine the therapeutic effects of melatonin on uveitis, a pellet of melatonin was implanted subcutaneously 2 hours before the intravitreal injection of either vehicle or LPS. Both 24 hours and 8 days after the injection, inflammatory responses were evaluated in terms of i) the integrity of the blood-ocular barrier, ii) clinical signs, iii) histopathological studies, and iv) retinal function. Melatonin reduced the leakage of proteins and cells in the anterior segment of LPS-injected eyes, decreased clinical signs such as dilation of the iris and conjunctival vessels, and flare in the anterior chamber, and protected the ultrastructure of the blood-ocular barrier. A remarkable disorganization of rod outer segment membranous disks was observed in animals injected with LPS, whereas no morphological changes in photoreceptor outer segments were observed in animals treated with melatonin. Furthermore, melatonin prevented a decrease in LPS-induced electroretinographic activity. In addition, melatonin significantly abrogated the LPS-induced increase in retinal nitric-oxide synthase activity, tumor necrosis factor-␣, and nuclear factor B p50 and p65 subunit levels. These results indicate that melatonin prevents the clinical, biochemical, histological, ultrastructural, and functional consequences of experimental uveitis, likely through a nuclear factor B-dependent mechanism, and support the use of melatonin as a new therapeutic strategy for the treatment of uveitis. (Am J Pathol

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Section: Discussionsupporting

confidence: 59%

Therapeutic Effect of Melatonin in Experimental Uveitis

Sande

Fernandez

Marcos

et al. 2008

The American Journal of Pathology

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“…27,28 Staining for cellular retinaldehyde-binding protein (a marker for Muller cells 29 ) showed no differences between rho/ PDGF-A2 or -A3 transgene-positive mice and wild-type mice (not shown).…”

Section: Rho/pdgf-a Mice Develop Retinal Gliosismentioning

confidence: 95%

Platelet-Derived Growth Factor-A-Induced Retinal Gliosis Protects against Ischemic Retinopathy

Yamada

Andõ

et al. 2000

The American Journal of Pathology

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“…23 Another effect of choroidal ischemia derived from the PGS implantation is GFAP upregulation in Mü ller cells, a sign of activation and glial remodeling known from vitrectomy procedures as well as from detachment. 24,25 After 7 days, GFAP-labeled Mü ller cells were normally arranged, but after 28 days, some Mü ller cells in the implantation area had undergone hypertrophy with processes extending into the subretinal space. A gliotic reaction in the subretinal space is not advantageous for retinal transplantation and may warrant modifications of the PGS membrane in future experiments.…”

Section: Discussionmentioning

confidence: 99%

Selective Removal of Photoreceptor CellsIn VivoUsing the Biodegradable Elastomer Poly(Glycerol Sebacate)

Ghosh

Neeley

Arnér

et al. 2011

Tissue Engineering Part A

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Retinal transplantation experiments have advanced considerably during recent years, but remaining diseased photoreceptor cells in the host retina physically obstruct the development of graft-host neuronal contacts that are required for vision. We here report selective removal of photoreceptors using the biodegradable elastomer poly(glycerol sebacate) (PGS). A 1Â3 mm PGS membrane was implanted in the subretinal space of normal rabbit eyes, and morphologic specimens were examined with hematoxylin and eosin staining and a panel of immunohistochemical markers. Seven days postoperatively, a patent separation of the neuroretina and retinal pigment epithelium was found as well as loss of several rows of photoreceptors in combination with massive terminal transferase-mediated dUTP nick-end labeling (TUNEL) staining for apoptosis in the outer nuclear layer. After 28 days, the neuroretina was reattached, the PGS membrane had degraded, and photoreceptors were absent in the implantation area. Activated Mü ller cells were found in the entire retina in 7-day specimens, and in the implantation area after 28 days. AII amacrine and rod bipolar cell morphology was not affected, except for disrupted dendritic branching, which was present in rod bipolar cells in 28-day specimens. We conclude that retinal detachment induced by the biodegradable PGS membrane creates a permissive environment in which graft-host neuronal connections may be facilitated in future retinal transplantation experiments.

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Glial fibrillary acidic protein increases in Müller cells after retinal detachment

Cited by 164 publications

References 24 publications

Therapeutic Effect of Melatonin in Experimental Uveitis

Therapeutic Effect of Melatonin in Experimental Uveitis

Platelet-Derived Growth Factor-A-Induced Retinal Gliosis Protects against Ischemic Retinopathy

Selective Removal of Photoreceptor CellsIn VivoUsing the Biodegradable Elastomer Poly(Glycerol Sebacate)

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