Türkiye, which has a complex climate structure, is shown among the countries that will be most affected by climate change with the effect of global warming. These effects will differ in different regions due to their topographic structure, location, and orographic characteristics. Trend analyzes are used to determine the direction and magnitude of this variability. In this study, trend analysis was carried out by using hydrometeorological data obtained from streamflow and meteorology stations located in the Karasu Sub-Basin located in the Euphrates-Tigris Basin. Three innovative trend methods, namely the Şen-Innovative Trend Analysis, Onyutha Trend Test, Combination of Wilcoxon Test and Scatter Diagram trend tests, were used together with the classical Mann-Kendall method in the monthly scale analysis of hydrometeorological data of the 1979-2020 period. Both graphical and statistical trend analysis can be done with innovative methods. The results obtained in the study, in which trend analyses were evaluated at the α=0.05 significance level, reveal significant and insignificant decreasing trends in the parameters of mean streamflow, maximum precipitation, total precipitation, mean and minimum relative humidity. Significant and insignificant increasing trends were determined in the maximum, minimum, and mean temperature, maximum relative humidity, and mean wind speed data. The trends obtained in the methods used in general are consistent with each other. The findings of this study could lead to a better knowledge of the region's hydrology and contribute sustainable water management. The trend analysis methods used in the study are thought to be quite helpful in the analysis of hydro-meteorological time series.