Global detection and identification of components from Yunnan Baiyao based on liquid chromatography hybrid ion trap time‐of‐flight mass spectrometry

Abstract

Yunnan Baiyao is a widely used herbal prescription in traditional medicine for the treatment of bleeding and hematological diseases, while its chemical profile remains elusive. In this work, a novel methodology combining polarity-directed extraction technique with a diagnostic ion filtering strategy based on LC hybrid ion trap TOF-MS analysis was developed for global, efficient, and rapid characterization of components in Yunnan Baiyao. Di-ethyl ether, n-butanol, and ethanol/water (70:30, v/v) covering low-to-high polarity ranges were chosen as the extraction solvent, respectively. The results clearly showed that, compared with conventional single extraction solvent, collaboratively using extraction solvents with different polarities can effectively increase the number of detected peaks and enrich the product ions information in multistage mass spectra analysis. By further matching diagnostic ions and fragmental pathways, a total of 34 components were successfully identified. Our work clearly demonstrates that integrating polarity-directed extraction and diagnostic ion filtering techniques is a powerful and reliable strategy for global detection and identification of complex chemicalome from herbal prescriptions, and may open new avenues for chemical analysis in other complex mixtures.

Chitosan-Based Composite Materials for Prospective Hemostatic Applications

“…Yunnan Baiyao , a well-known herb prescription used in oriental countries for more than 100 years, is an effective surgical sealant and hemostat. However, it is not suitable for direct intraluminal application due to the lack of an ideal substrate [21,65,66]. Lu et al [29] prepared chitosan/sodium alginate- Yunnan Baiyao composite hemostatic films by blending Yunnan Baiyao with CS and sodium alginate.…”

Yunnan Baiyao Conditioned Medium Promotes the Odonto/Osteogenic Capacity of Stem Cells from Apical Papilla via Nuclear Factor Kappa B Signaling Pathway

“…Traditional Chinese medicine (TCM) plays a significant role in the treatment of tooth ache, bone fracture, and osteoarthritis in many oriental countries for a very long lapse of time [9, 10]. Yunnan Baiyao (YNB), which was formulated in 1902, has complex components including radix notoginseng, forest musk, radix aconiti kusnezoffii, and rhizoma paridis chonglou [11, 12]. It is well-known in China for its numerous clinical applications such as vasodilation, anti-inflammation, wound healing, and regeneration of damaged bones [13].…”

Herb Sanqi-Derived Compound K Alleviates Oxidative Stress in Cultured Human Melanocytes and Improves Oxidative-Stress-Related Leukoderma in Guinea Pigs

“…In addition, it has been used extensively in medical research or clinical settings for a number of disorders, including hypertension, atherosclerosis, diabetes, acute lung injury, cancer, and cardiovascular diseases. Injections and capsules of Sanqi extract have become commercially available and are widely used in clinical practice [30,31]. This agent attracted our attention because Sanqi-CK has been recently reported to be beneficial in various oxidative-stress-related conditions.…”