Global DNA methylation variations after short-term heat shock treatment in cultured microspores of Brassica napus cv. Topas

Li, Jun; Huang, Qian; Sun, Mingwei; Wang, Ziyang; Li, Hao; Chen, Biyun; Xu, Kun; Gao, Guizhen; Li, Feng; Yan, Guiqin; Qiao, Jiangwei; Yongping, Cai; Wu, Xiaoming

doi:10.1038/srep38401

Cited by 66 publications

(50 citation statements)

References 51 publications

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“…For all the sequences, the methylation density under the CG context was the highest, followed by that under the CHG and CHH contexts, which is consistent with two other reports on rapeseed [ 1 , 35 ]. The DNA methylation level varies among different tissues and organs.…”

Section: Discussionsupporting

confidence: 91%

“…Chalhoub et al observed that C n genes presented 4% to 8% higher methylation than their homologous A n genes from the methyl bisulfite sequencing of roots and leaves [ 1 ]. A similar result was obtained in cultured microspores by Li et al: the amount of methylated cytosines distributed on the C n subgenome was obviously larger than that distributed on the A n subgenome [ 35 ]. It was hypothesized that the different DNA methylation levels between the A n and C n subgenomes can be attributed to the asymmetrical distribution of TE density in the genome of B. napus .…”

Section: Discussionsupporting

confidence: 82%

“…Compared with roots and leaves [ 1 ], the average methylation level was lower in young floral buds, especially under the CHH context ( Figure S3 ). Li et al determined the mean methylation level in cultured microspores under all three contexts [ 35 ], which was also lower than that in the roots and leaves. Considering the general role of DNA methylation in silencing genes, the lower methylation level indicated active gene expression in the reproductive organs.…”

Section: Discussionmentioning

confidence: 99%

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Genome-Wide DNA Methylation Comparison between Brassica napus Genic Male Sterile Line and Restorer Line

Wang

et al. 2018

IJMS

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DNA methylation is an essential epigenetic modification that dynamically regulates gene expression during plant development. However, few studies have determined the DNA methylation profiles of male-sterile rapeseed. Here, we conducted a global comparison of DNA methylation patterns between the rapeseed genic male sterile line 7365A and its near-isogenic fertile line 7365B by whole-genome bisulfite sequencing (WGBS). Profiling of the genome-wide DNA methylation showed that the methylation level in floral buds was lower than that in leaves and roots. Besides, a total of 410 differentially methylated region-associated genes (DMGs) were identified in 7365A relative to 7365B. Traditional bisulfite sequencing polymerase chain reaction (PCR) was performed to validate the WGBS data. Eleven DMGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. In particular, Bnams4 was hypo-methylated in 7365A, and its expression was up-regulated, which might affect other DMGs and thus control the male sterility. This study provided genome-wide DNA methylation profiles of floral buds and important clues for revealing the molecular mechanism of genic male sterility in rapeseed.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

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Genome-Wide DNA Methylation Comparison between Brassica napus Genic Male Sterile Line and Restorer Line

Wang

et al. 2018

IJMS

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“…oleracea methylome [ 49 ]. Further, only a limited number of studies have assessed the potential involvement of altered DNA methylation under heat stress [ 50 , 51 , 52 ].…”

Section: Introductionmentioning

confidence: 99%

The DNA Methylome and Association of Differentially Methylated Regions with Differential Gene Expression during Heat Stress in Brassica rapa

Liu

Xia

Hou

et al. 2018

IJMS

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Cytosine DNA methylation is a critical epigenetic mechanism in the silencing of transposable elements, imprinting and regulating gene expression. However, little is known about the potential role of mC in response to heat stress. To determine and explore the functions of the dynamic DNA methylome during heat stress, we characterized single-base resolution methylome maps of Brassica rapa and assessed the dynamic changes of mC under heat stress using whole genome bisulfite sequencing. On average, the DNA methylation levels of CG, CHG and CHH are 39.3%, 15.38% and 5.24% in non-heading Chinese cabbage (NHCC), respectively. We found that the patterns of methylation are similar to other eudicot plants, but with higher CHH methylation levels. Further comparative analysis revealed varying patterns for three sequence contexts (mCG, mCHG and mCHH) under heat stress indicating context- and position-dependent methylation regulation. DNA methylation near the TSS and TES may be closely associated with methylation-dependent transcriptional silencing. Association analysis of differential methylation and differential gene expression revealed a different set of methDEGs involved at early and late stages under heat stress. The systemic characterization of the dynamic DNA methylome during heat stress will improve our understanding of the mechanism of epigenetic regulation under heat stress.

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“…Methylation enhanced more in the heat-sensitive genotype than in the heat tolerant genotype under heat treatments. It is reported that more changes in the DNA methylation status of cultured microspores were observed under high temperature [259].…”

Section: Epigenetics and Heat Tolerancementioning

confidence: 99%

Unraveling Field Crops Sensitivity to Heat Stress: Mechanisms, Approaches, and Future Prospects

Nadeem

Wang

et al. 2018

Agronomy

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The astonishing increase in temperature presents an alarming threat to crop production worldwide. As evident by huge yield decline in various crops, the escalating drastic impacts of heat stress (HS) are putting global food production as well as nutritional security at high risk. HS is a major abiotic stress that influences plant morphology, physiology, reproduction, and productivity worldwide. The physiological and molecular responses to HS are dynamic research areas, and molecular techniques are being adopted for producing heat tolerant crop plants. In this article, we reviewed recent findings, impacts, adoption, and tolerance at the cellular, organellar, and whole plant level and reported several approaches that are used to improve HS tolerance in crop plants. Omics approaches unravel various mechanisms underlying thermotolerance, which is imperative to understand the processes of molecular responses toward HS. Our review about physiological and molecular mechanisms may enlighten ways to develop thermo-tolerant cultivars and to produce crop plants that are agriculturally important in adverse climatic conditions. 128 2 of 34 and osmotic adjustment and re-establish the redox balance of cell and homeostasis by modify the antioxidant system [10,11]. A plant in defense from HS causes modifications at the molecular level in the expression of genes [12]. In HS conditions, change in biochemical and physiological activities by gene expression alter gradually, resulting in the development of thermotolerance [13]. In order to successfully produce HS-tolerant crop varieties in the light of global climate change, there is need of knowledge and investigations about HS-tolerance mechanisms at physiological, biochemical, and molecular levels.At present, investigations into selection strategy and breeding for thermotolerant cultivars and understanding of heat tolerance mechanisms are more required today than ever before. Molecular and genetic mechanisms for avoiding HS-induced harmful changes play a crucial role in plant survival under such circumstances. In the present scenario of global warming, the major challenge for plant scientists is to develop new crop varieties tolerant to HS [14]. In the coming years, agricultural production will have to deal with growing crops under sub-optimal conditions accompanied by increased food demand, creating a gap between the current yield achievements and yield potential [15]. Developing genetically modified plants through target genes manipulation, QTLs, and omics techniques are widely studied molecular approaches in recent years. Our study about sensitivity, adaptations, mechanisms, and approaches may uncover ways to develop thermo-tolerant cultivars and to produce crop plants that are agriculturally important in adverse climatic conditions. Plant Sensitivity to Heat StressPlant sensitivity to HS varies with duration, plant type, and the degree of temperature. Plant growth and development are greatly influenced by the series of morphological, physiological, and biochemical changes resul...

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Global DNA methylation variations after short-term heat shock treatment in cultured microspores of Brassica napus cv. Topas

Cited by 66 publications

References 51 publications

Genome-Wide DNA Methylation Comparison between Brassica napus Genic Male Sterile Line and Restorer Line

Genome-Wide DNA Methylation Comparison between Brassica napus Genic Male Sterile Line and Restorer Line

The DNA Methylome and Association of Differentially Methylated Regions with Differential Gene Expression during Heat Stress in Brassica rapa

Unraveling Field Crops Sensitivity to Heat Stress: Mechanisms, Approaches, and Future Prospects

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