Global gene expression changes in BV2 microglial cell line during rabies virus infection

Zhao, Pingsen; Yang, Yujiao; Feng, Hao; Zhao, Lili; Qin, Junling; Zhang, Tao; Wang, Hualei; Wang, Cuiling; Xia, Xianzhu

doi:10.1016/j.meegid.2013.09.016

Cited by 25 publications

(20 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…24,25 Several in vivo or in vitro studies have demonstrated that CXCL10 is present and upregulated after RABV infection. 6,22,46 This chemokine has been shown to play an important role in BBB permeability and seems to be essential in the clearance of RABV from the CNS because it allows the entry of immune effector cells. 40 Although studies 6,40 have described enhanced expression of CXCL10 in models infected with attenuated RABV, but not in those infected with wild-type RABV, the results of this study revealed a statistically significant upregulation of CXCL10 and TNF-α in animals infected with wild-type strains.…”

Section: Resultsmentioning

confidence: 99%

Profile of Cytokines and Chemokines Triggered by Wild-Type Strains of Rabies Virus in Mice

Appolinário

Allendorf

Peres

et al. 2016

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Rabies is a lethal infectious disease that causes 55,000 human deaths per year and is transmitted by various mammalian species, such as dogs and bats. The host immune response is essential for avoiding viral progression and promoting viral clearance. Cytokines and chemokines are crucial in the development of an immediate antiviral response; the rabies virus (RABV) attempts to evade this immune response. The virus's capacity for evasion is correlated with its pathogenicity and the host's inflammatory response, with highly pathogenic strains being the most efficient at hijacking the host's defense mechanisms and thereby decreasing inflammation. The purpose of this study was to evaluate the expression of a set of cytokine and chemokine genes that are related to the immune response in the brains of mice inoculated intramuscularly or intracerebrally with two wild-type strains of RABV, one from dog and the other from vampire bat. The results demonstrated that the gene expression profile is intrinsic to the specific rabies variant. The prompt production of cytokines and chemokines seems to be more important than their levels of expression for surviving a rabies infection.

show abstract

Section: Resultsmentioning

confidence: 99%

Profile of Cytokines and Chemokines Triggered by Wild-Type Strains of Rabies Virus in Mice

Appolinário

Allendorf

Peres

et al. 2016

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

“…Concomitant activation of JAK/STAT and AKT signaling pathways has a pro-survival function in neurons (Junyent et al, 2010). In a time-course study, Zhao et al studied the gene expression profile of infected microglial cells and indicated some affected signaling pathways at different time points (Zhao et al, 2013). The MAPK, chemokine, and JAK-STAT signaling were also shown to be implicated in rabies infection along with other innate and adaptive immune response pathways.…”

Section: Resultsmentioning

confidence: 99%

“…Gene expression analysis of a number of randomly selected DEGs has been performed previously based on RT-qPCR as a routine validation method of microarray-based expression profiles (Zhao et al, 2011, 2012a,b, 2013) or fluorescent bead immunoassay (Sugiura et al, 2011). In all cases, the former experimentally confirmed DEGs identified in SHIDEGs were STAT1, STAT3, SOCS2, IRF1, IRF3, IRF7, IFNAR2, SH2D1A, CCL3, CCL5, CCRL2, CXCL10, Mx1, IFIT3, OASL2, USP18, IL6, IL10, IL23A , and RTP4 .…”

Section: Resultsmentioning

confidence: 99%

“…Elucidating the essential biological processes involved in rabies progression has been based mainly on analyzing gene expression alterations. Zhao et al reported expression profiling of mRNA and microRNA of rabies-infected cell (Zhao et al, 2011, 2012a,b, 2013). Suigiura et al analyzed the gene expression profile of CNS tissue infected with CVS-11 (Sugiura et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Systems Biomedicine of Rabies Delineates the Affected Signaling Pathways

et al. 2016

View full text Add to dashboard Cite

The prototypical neurotropic virus, rabies, is a member of the Rhabdoviridae family that causes lethal encephalomyelitis. Although there have been a plethora of studies investigating the etiological mechanism of the rabies virus and many precautionary methods have been implemented to avert the disease outbreak over the last century, the disease has surprisingly no definite remedy at its late stages. The psychological symptoms and the underlying etiology, as well as the rare survival rate from rabies encephalitis, has still remained a mystery. We, therefore, undertook a systems biomedicine approach to identify the network of gene products implicated in rabies. This was done by meta-analyzing whole-transcriptome microarray datasets of the CNS infected by strain CVS-11, and integrating them with interactome data using computational and statistical methods. We first determined the differentially expressed genes (DEGs) in each study and horizontally integrated the results at the mRNA and microRNA levels separately. A total of 61 seed genes involved in signal propagation system were obtained by means of unifying mRNA and microRNA detected integrated DEGs. We then reconstructed a refined protein–protein interaction network (PPIN) of infected cells to elucidate the rabies-implicated signal transduction network (RISN). To validate our findings, we confirmed differential expression of randomly selected genes in the network using Real-time PCR. In conclusion, the identification of seed genes and their network neighborhood within the refined PPIN can be useful for demonstrating signaling pathways including interferon circumvent, toward proliferation and survival, and neuropathological clue, explaining the intricate underlying molecular neuropathology of rabies infection and thus rendered a molecular framework for predicting potential drug targets.

show abstract

“…Efforts to explore the underlying mechanisms have been reported, and several research groups have conducted multi-omics studies, including transcriptomics, microRNomics, and proteomics analyses, to elucidate the major host factors associated with the pathogenesis of different strains [7–11]. Zhao et al performed a microarray analysis to reveal the different transcript and microRNA profiles of mouse brains infected with street or laboratory-fixed RABV [9, 10, 12, 13]. Through two-dimensional electrophoresis, Hu et al demonstrated that the overexpression of certain cytoskeletal proteins in neuroblastoma (NA) cells is positively correlated with the virulence of RABV [11].…”

Section: Introductionmentioning

confidence: 99%

Autophagy is highly targeted among host comparative proteomes during infection with different virulent RABV strains

Jin

Wang

et al. 2017

Oncotarget

Self Cite

View full text Add to dashboard Cite

Rabies virus (RABV) is a neurotropic virus that causes serious disease in humans and animals worldwide. It has been reported that different RABV strains can result in divergent prognoses in animal model. To identify host factors that affect different infection processes, a kinetic analysis of host proteome alterations in mouse brains infected with different virulent RABV strains was performed using isobaric tags for a relative and absolute quantification (iTRAQ)-liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomics approach, and this analysis identified 147 differentially expressed proteins (DEPs) between the pathogenic challenge virus standard (CVS)-11 strain and the attenuated SRV9 strain. Bioinformatics analyses of these DEPs revealed that autophagy and several pathways associated with autophagy, such as mammalian target of rapamycin (mTOR) signaling, p70S6K signaling, nuclear factor erythroid 2-related factor 2 (NRF2)-mediated oxidative stress and superoxide radical degradation, were dysregulated. Validation of the proteomic data showed that attenuated SRV9 induced more autophagosome accumulation than CVS-11 in an in vitro model. Our findings provide new insights into the pathogenesis of RABV and encourage further studies on this topic.

show abstract

Global gene expression changes in BV2 microglial cell line during rabies virus infection

Cited by 25 publications

References 76 publications

Profile of Cytokines and Chemokines Triggered by Wild-Type Strains of Rabies Virus in Mice

Profile of Cytokines and Chemokines Triggered by Wild-Type Strains of Rabies Virus in Mice

Systems Biomedicine of Rabies Delineates the Affected Signaling Pathways

Autophagy is highly targeted among host comparative proteomes during infection with different virulent RABV strains

Contact Info

Product

Resources

About