Global microRNA profiling for diagnostic appraisal of melanocytic Spitz tumors

Latchana, Nicholas; Regan, Kelly; Howard, John H.; Aldrink, Jennifer H.; Ranalli, Mark; Peters, Sara; Zhang, Xiaoli; Gru, Alejandro A.; Payne, Philip R. O.; Suarez‐Kelly, Lorena P.; Carson, William E.

doi:10.1016/j.jss.2016.06.085

Cited by 19 publications

(15 citation statements)

References 40 publications

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“…In this study, we observed that the expression of miR‐21‐5p is up‐regulated in melanoma tissues. Consistently, increased miR‐21 expression has been observed in malignant melanocytic skin tissues compared with benign tumors [12‐14]. Different from the study by Martin del Campo et al .…”

Section: Discussionmentioning

confidence: 70%

“…Interestingly, increased miR‐21 expression has been observed during the transition from a benign melanocytic lesion to malignant melanoma [12]. Similarly, miR‐21‐5p has been reported to be overexpressed in malignant melanocytic skin tissues compared with benign tumors by global miRNA profiling [13], as well as next‐generation sequencing by Babapoor et al [14] and Latchana et al [2]. Functionally, miR‐21 has been demonstrated to enhance the invasiveness of melanoma cells by inhibition of tissue inhibitor of metalloproteinases 3 [15].…”

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confidence: 99%

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miR‐21‐5p promotes cell proliferation and G1/S transition in melanoma by targeting CDKN2C

et al. 2020

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Human melanoma is a highly malignant tumor originating from cutaneous melanocytes. The noncoding RNA microRNA (miR)‐21‐5p has been reported to be expressed at high levels in malignant melanocytic skin tissues, but its potential functional role in melanoma remains poorly understood. Here, we explored the cellular effects of miR‐21‐5p on melanoma in vitro and the underlying mechanisms. Quantitative real‐time PCR was used to show that miR‐21‐5p is significantly up‐regulated in clinical samples from patients with melanoma as compared with adjacent noncancerous tissues. Overexpression of miR‐21‐5p significantly enhanced, whereas knockdown attenuated, cell proliferation and G1/S transition in melanoma cell lines (A375 and M14). Luciferase reporter assays were used to show that the cyclin‐dependent kinase inhibitor 2C (CDKN2C) is a downstream target of miR‐21‐5p. Furthermore, miR‐21‐5p mimics resulted in a decrease in CDKN2C expression, and CDKN2C expression was observed to be inversely correlated with miR‐21‐5p expression in melanoma tissues. Rescue experiments were performed to show that overexpression of CDKN2C partially reversed the effects of miR‐21‐5p up‐regulation on A375 cells. Consistently, knockdown of CDKN2C abolished the effects of miR‐21‐5p down‐regulation on A375 cells. Overall, our studies demonstrate that miR‐21‐5p can promote the growth of melanoma cells by targeting CDKN2C, which may induce G0/G1 phase arrest of melanoma cells.

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Section: Discussionmentioning

confidence: 70%

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confidence: 99%

miR‐21‐5p promotes cell proliferation and G1/S transition in melanoma by targeting CDKN2C

et al. 2020

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“…In addition, miR-1260b was investigated in malignant melanoma patients, and miR-1260a was reported to show a different expression pattern in the surgical resection follow up of melanoma [64]. mi1260a expression was shown to have higher expression in atypical Spitz lesions compared with the levels in benign Spitz tumors [65]. mi1260b was detected to decrease the cell migration, and invasion in nonsmall cell lung cancer cell strains (A549).…”

Section: Discussionmentioning

confidence: 99%

High Expression Level of miR-1260 Family in the Peripheral Blood of Patients with Ovarian Carcinoma

Ghafour

Ödemiş

Tunçer

et al. 2020

Preprint

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The most common gynecologic cancers detected in women in Turkey are uterine cancer, ovarian cancer, and cervical cancer. These data reported that a mean of 3800 individuals were diagnosed with uterine cancer, 2790 were diagnosed with ovarian cancer, and 1950 were diagnosed with cervical cancer, and 400 individuals were diagnosed with other gynecologic cancers each year in Turkey. A mean of 14.270 individuals were detected to have been diagnosed with gynecologic cancers each year in the United States of America(USA). Ovarian cancer treatment is generally composed of chemotherapy, and surgery. In general, chemotherapy is administered after surgery. The identification of the molecular pathogenesis of ovarian cancer, and discovery of new moleculer biomarkers which facilitate the ovarian cancer treatment are required for an effective ovarian cancer treatment in clinics. miRNAs are reported to be the possible biologic indicators for various cancer types. We aimed to investigate 2 miRNAs which were suggested to have effect in ovarian cancer in our (previous)monozygotic twin study from miR-1260 microRNA family whose association with ovarian cancer yet has not been reported in the literature. We investigated the expression levels of miR-1260a, and miR-1260b miRNAs, in the peripheral blood lymphocytes of 150 familial and sporadic ovarian cancer patients, and of 100 healthy individuals of the control group who were matched for age, sex, and ethnicity with the patient group, and investigated their possible property of being a biologic indicator for ovarian cancer. The expression results of ovarian cancer patients were evaluated by comparison of the results of the control group in the study. The expression levels of miR-1260a, and miR-1260b in ovarian cancer patients were found highly increased compared with the levels in the control group. miR-1260a expression level in ovarian cancer patients was detected to have increased approximately 17 fold compared with the control group, and miR-1260b expression level in ovarian cancer patients was detected to have increased approximately 33 fold compared with the levels in the control group. The String Analyses showed that the miR-1260a was associated with the ribosomal protein family which was known to be effective in the translation stage of cell and that miR-1260b was associated with CHEK2 protein which was a member of the serine/threonine-protein kinase family. It should be investigated for larger cohorts in benign ovarian diseases and in different stages of patients receiving ovarian cancer treatment whether these two molecules are a noninvasive biomarker and therapeutic target to be used especially in the early diagnosis and prognosis of ovarian cancer in future.

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“…Another miRNA, hsa-miR-484, is involved in apoptosis suppression [49]. Moreover, we decided to include in our quantitative analysis hsa-miR-1260a because of its prognostic value in a few cancer types [50][51][52].…”

Section: Overexpression Of Mcpip1 Protein Does Not Significantly Altementioning

confidence: 99%

MCPIP1 ribonuclease can bind and cleave AURKA mRNA in MYCN -amplified neuroblastoma cells

et al. 2020

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The role of the inflammation-silencing ribonuclease, MCPIP1 (monocyte chemoattractant proteininduced protein 1), in neoplasia continuous to emerge. The ribonuclease can cleave not only inflammation-related transcripts but also some microRNAs (miRNAs) and viral RNAs. The suppressive effect of the protein has been hitherto suggested in breast cancer, clear cell renal cell carcinoma, osteosarcoma, and neuroblastoma. Our previous results have demonstrated a reduced levels of several oncogenes, as well as inhibited growth of neuroblastoma cells upon MCPIP1 overexpression. Here, we investigate the mechanisms underlying the suppression of MYCN proto-oncogene, bHLH transcription factor (MYCN)amplified neuroblastoma cells overexpressing the MCPIP1 protein. We showed that the levels of several transcripts involved in cell cycle progression decreased in BE(2)-C and KELLY cells overexpressing MCPIP1 in a ribonucleolytic activity-dependent manner. However, RNA immunoprecipitation indicated that only AURKA mRNA (encoding for Aurora A kinase) interacts with the ribonuclease. Furthermore, the application of a luciferase assay suggested MCPIP1-dependent destabilization of the transcript. Further analyses demonstrated that the entire conserved region of AURKA seems to be indispensable for the interaction with the MCPIP1 protein. Additionally, we examined the effect of the ribonuclease overexpression on the miRNA expression profile in MYCN-amplified neuroblastoma cells. However, no significant alterations were observed. Our data indicate a key role of the binding and cleavage of the AURKA transcript in an MCPIP1-dependent suppressive effect on neuroblastoma cells.

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Global microRNA profiling for diagnostic appraisal of melanocytic Spitz tumors

Cited by 19 publications

References 40 publications

miR‐21‐5p promotes cell proliferation and G1/S transition in melanoma by targeting CDKN2C

miR‐21‐5p promotes cell proliferation and G1/S transition in melanoma by targeting CDKN2C

High Expression Level of miR-1260 Family in the Peripheral Blood of Patients with Ovarian Carcinoma

MCPIP1 ribonuclease can bind and cleave AURKA mRNA in MYCN -amplified neuroblastoma cells

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