Global population genetic structure of<i>Bean common mosaic virus</i>

Abadkhah, Mahsa; Hajizadeh, Mohammad Reza; Koolivand, Davoud

doi:10.1080/03235408.2020.1743525

Cited by 9 publications

(5 citation statements)

References 33 publications

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“…Sequences obtained by Sanger were used for assignment to pathotypes/subgroups according to recent comprehensive studies of BCMV [ 27 ], CMV [ 28 ], and PSV [ 29 ]. The results revealed that 79% of BCMV sequences fall into pathotype VII, sharing 97.7–99.2% identity with NL-4 strain (DQ666332) [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

“…To compare with previous phylogenetic works in the literature, reference sequences of strains detected in this investigation were aligned and analyzed with those included in the most recent phylogenetic studies on BCMV [ 27 ], CMV [ 28 ] and PSV [ 29 ]. Phylogeny of BCMV, PSV and CMV isolates infecting Lamon bean plants was reconstructed by adopting the maximum likelihood (ML) method as implemented in Geneious—R10 (Biomatters Ltd., Auckland, New Zealand).…”

Section: Methodsmentioning

confidence: 99%

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The Virome of ‘Lamon Bean’: Application of MinION Sequencing to Investigate the Virus Population Associated with Symptomatic Beans in the Lamon Area, Italy

Tarquini

Martini

Maestri

et al. 2022

Plants

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‘Lamon bean’ is a protected geographical indication (PGI) for a product of four varieties of bean (Phaseolus vulgaris L.) grown in a specific area of production, which is located in the Belluno district, Veneto region (N.E. of Italy). In the last decade, the ‘Lamon bean’ has been threatened by severe virus epidemics that have compromised its profitability. In this work, the full virome of seven bean samples showing different foliar symptoms was obtained by MinION sequencing. Evidence that emerged from sequencing was validated through RT-PCR and ELISA in a large number of plants, including different ecotypes of Lamon bean and wild herbaceous hosts that may represent a virus reservoir in the field. Results revealed the presence of bean common mosaic virus (BCMV), cucumber mosaic virus (CMV), peanut stunt virus (PSV), and bean yellow mosaic virus (BYMV), which often occurred as mixed infections. Moreover, both CMV and PSV were reported in association with strain-specific satellite RNAs (satRNAs). In conclusion, this work sheds light on the cause of the severe diseases affecting the ‘Lamon bean’ by exploitation of MinION sequencing.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The Virome of ‘Lamon Bean’: Application of MinION Sequencing to Investigate the Virus Population Associated with Symptomatic Beans in the Lamon Area, Italy

Tarquini

Martini

Maestri

et al. 2022

Plants

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“…Plant RNA viruses have the indisputable potential for genetic variety, fast development, and adaptability to new environmental conditions ( Abadkhah et al, 2020 ). BCMV, a typical member of Potyviruses , has all the features of the genus, such as high mutation ability, recombination, and formation of various strains ( Gibbs et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

A novel study on bean common mosaic virus accumulation shows disease resistance at the initial stage of infection in Phaseolus vulgaris

et al. 2023

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Accurate and early diagnosis of bean common mosaic virus (BCMV) in Phaseolus vulgaris tissues is critical since the pathogen can spread easily and have long-term detrimental effects on bean production. The use of resistant varieties is a key factor in the management activities of BCMV. The study reported here describes the development and application of a novel SYBR Green-based quantitative real-time PCR (qRT-PCR) assay targeting the coat protein gene to determine the host sensitivity to the specific NL-4 strain of BCMV. The technique showed high specificity, validated by melting curve analysis, without cross-reaction. Further, the symptoms development of twenty advanced common bean genotypes after mechanical BCMV-NL-4 infection was evaluated and compared. The results showed that common bean genotypes exhibit varying levels of host susceptibility to this BCMV strain. The YLV-14 and BRS-22 genotypes were determined as the most resistant and susceptible genotypes, respectively, in terms of aggressiveness of symptoms. The accumulation of BCMV was analyzed in the resistant and susceptible genotypes 3, 6, and 9 days following the inoculation by the newly developed qRT-PCR. The mean cycle threshold (Ct) values showed that the viral titer was significantly lower in YLV-14, which was evident in both root and leaf 3 days after the inoculation. The qRT-PCR thus facilitated an accurate, specific, and feasible assessment of BCMV accumulation in bean tissues even in low virus titers, allowing novel clues in selecting resistant genotypes in the early stages of infection, which is critical for disease management. To the best of our knowledge, this is the first study of a successfully performed qRT-PCR to estimate BCMV quantification.

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“…It is well known that mutation is the key factor driving the rapid evolution of RNA viruses and generating genetic diversity to adopt different hosts and environments for survival and transmission [113][114][115][116][117][118]. The situation is the same as that of BCMV: it has been shown that mutation is the major factor allowing BCMV to quickly overcome multiple resistance genes in the common bean [109,119,120].…”

Section: Evolutionmentioning

confidence: 99%

Bean Common Mosaic Disease: Etiology, Resistance Resource, and Future Prospects

Tang

Feng

2022

Agronomy

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Bean common mosaic disease is one of the most destructive diseases of the common bean, which is one of the most important legumes worldwide. It is caused by two closely related potyviruses: bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV). Both viruses have spread to all the common bean-growing areas worldwide and have become a major challenge in bean production. In this review, we summarized the biology and diversity of BCMV and BCMNV, discussed the current knowledge on the resistance genes of BCMV, and finally pointed out the future prospects for the control of bean common mosaic disease.

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Global population genetic structure ofBean common mosaic virus

Cited by 9 publications

References 33 publications

The Virome of ‘Lamon Bean’: Application of MinION Sequencing to Investigate the Virus Population Associated with Symptomatic Beans in the Lamon Area, Italy

The Virome of ‘Lamon Bean’: Application of MinION Sequencing to Investigate the Virus Population Associated with Symptomatic Beans in the Lamon Area, Italy

A novel study on bean common mosaic virus accumulation shows disease resistance at the initial stage of infection in Phaseolus vulgaris

Bean Common Mosaic Disease: Etiology, Resistance Resource, and Future Prospects

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