Global prevalence, characteristics, and future prospects of IncX3 plasmids: A review

Guo, Xia; Chen, Ruyan; Wang, Qian; Li, Chenyu; Ge, Haoyu; Qiao, Jie; Li, Yuan

doi:10.3389/fmicb.2022.979558

Cited by 27 publications

(28 citation statements)

References 62 publications

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“…In our study, most IncX plasmids were IncX3, deemed the primary vehicle for bla NDM transmission. IncX3 plasmids have been found to spread rapidly among different species without tness cost, with excellent conjugation ability, high stability, and the ability to improve bio lm formation in their bacterial hosts [45]. Given that IncX3 plasmids were found among most of the bla NDM -carrying E. coli in our study, we speculate that IncX is the main host for bla NDM [46].…”

Section: Discussionmentioning

confidence: 67%

Genomic characteristics of clinical carbapenem-resistant Escherichia coli isolates in a tertiary hospital, 2013–2021

Xie

Han

et al. 2023

Preprint

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Background: Genomic characterization of carbapenem-resistant Escherichia coli (CREC) isolates is necessary to understand its transmission and implement infection control measures, given its potential threat to public health. Method：A total of 100 CREC isolates collected from 2013–2021 at a Nanjing tertiary hospital were analyzed using whole-genome sequencing (WGS). The distribution of resistant determinants (RTs), virulence factors (VFs), multi-locus sequence type (MLST), and FimH types was determined. Furthermore, a phylogenetic tree was constructed to investigate the genetic relationships. Result:All CREC strains (n=100) carried at least one carbapenem hydrolyzing ß-lactamase (CHßL)-encoding gene, with blaNDM (n=83, 83.0%) being the most common. Multiple RTs were detected with blaCTX (n=83, 83.0%) and blaTEM (n=78, 78.0%) being the most dominant ones. MLST identified 37 distinct STs, with ST167 (n=18, 18.0%) and ST410 (n=13, 13.0%) being the most predominant clones. Multiple plasmid replicons were found. The strains displayed 64 different virulence factors, with terC (n=98, 98.0%) and traT (n=76, 76.0%) being the most frequent. Genetic diversity was observed between the 100 CREC isolates, with clonal dissemination of ST167, ST361, ST2179, ST410, and ST131 being observed. Conclusion: The prevalence of blaNDM-5, a major blaCHßLs, is concerning due to its distribution of various co-carried resistance genes and their potential to spread through multiple plasmid replicons. The isolates exhibit genetic diversity based on STs, Fim typing, serotyping, and phylogenetic tree analysis. ST167, ST361, ST2179, ST410, and ST131 are linked to clonal dissemination, highlighting the need for effective infection control measures to prevent their spread.

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Section: Discussionmentioning

confidence: 67%

Genomic characteristics of clinical carbapenem-resistant Escherichia coli isolates in a tertiary hospital, 2013–2021

Xie

Han

et al. 2023

Preprint

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“…According to Wang et al (2018), IncX3 plasmids had similar or higher conjugation frequencies than IncFII plasmids at 30°C and 37°C, suggesting that the plasmid replicon types are associated with temperature in the horizontal transfer of antibiotic resistance genes (Wang et al, 2018). IncX3 plasmids can be transferred between various Enterobacterial species over a wide temperature range (Guo et al, 2022). Previous studies reported higher frequencies of plasmid delivery to recipients at 30°C than at 25°C or 37°C (Liakopoulos et al, 2018;Walsh et al, 2011), and that a temperature of 30°C increases conjugation (Anaı¨s Potron et al, 2011).…”

Section: Discussionmentioning

confidence: 97%

“…Among the incompatible plasmid groups associated with carbapenemase genes in Enterobacterales (Katlego Kopotsa et al, 2019), the bla KPC gene is harbored by IncF, IncI2, IncX, IncA/C, IncR, and ColE1 , whereas bla NDM is mainly located on plasmids of the IncX3 type (Liu et al, 2021). The IncX3 plasmid transports and spreads carbapenemase genes (bla NDM , bla KPC , and bla OXA−48 -like), particularly bla NDM (Guo et al, 2022). In our study, IncF was dominant for the bla KPC gene, and IncX3 was predominant for bla NDM (Tables 2 and 3).…”

Section: Discussionmentioning

confidence: 99%

Temperature effect on carbapenemase-encoding plasmid transfer

Yang,

Nam,

Lee

et al. 2023

Preprint

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Bacteria that cause human infections can acquire antibiotic resistance, and several factors may play a role. Although temperature is known to affect bacterial growth in vitro and regulate the transfer of genes encoding antibiotic resistance, there is little evidence of changes in antibiotic resistance with ambient temperature. We investigated the distribution of antibiotic resistance at different ambient temperatures and evaluated whether temperature affected the transmission of carbapenemase-producing Enterobacterales (CPE). The study on Klebsiella pneumoniae from eight sentinel hospitals of the Korea Global Antimicrobial Resistance Surveillance System (Kor-GLASS) between 2017 and 2021 was included in the analysis. Conjugation experiments were performed at different temperatures for strains harboring representative carbapenemase genes. The resistance rates to most antibiotics, including carbapenems, varied significantly according to ambient temperature (P < 0.047), except for aminoglycosides. The optimal conjugation temperature for blaKPC-carrying plasmids was 25°C (P = 0.030) and plasmids carrying blaNDM showed the highest conjugation frequency at 30°C (P = 0.007). The blaKPC-IncF showed higher stability at 25°C than at 30°C (P = 0.032) or 37°C (P = 0.047), and the plasmid stability of blaKPC-IncX3 was the lowest at 37°C (P = 0.047). The blaNDM-IncF was very stable at 30°C, and blaNDM-IncX3 was the highest at 30°C and the lowest at 37°C (P = 0.049). In conclusion, we confirmed that carbapenemase gene transmission was optimal at 25–30°C. This suggests that more antibiotic resistance-related genes can be transferred in warmer seasons. Therefore, we suggest that ambient temperature plays an important role in the spread and transfer of CPE.

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“…This suggests that with further study and discovery, PDPs could be exploited to manipulate the dynamics of conjugative plasmid mobility, and thus the spread of antibiotic resistance genes in high-risk environments. PDPs may be particularly applicable to controlling epidemics of plasmid acquired resistance, for example the current epidemic of carbapenem-resistant Enterobacteriaceae mobilized by IncX3 conjugative plasmids [52][53][54] .…”

Section: Discussionmentioning

confidence: 99%

Diverse and abundant phages exploit conjugative plasmids

Quinones-Olvera

Owen

McCully

et al. 2023

Preprint

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Viruses exert profound evolutionary pressure on bacteria by interacting with receptors on the cell surface to initiate infection. While the majority of bacterial viruses, phages, use chromosomally-encoded cell surface structures as receptors, plasmid dependent-phages exploit plasmid-encoded conjugation proteins, making their host range dependent on horizontal transfer of the plasmid. Despite their unique biology and biotechnological significance, only a small number of plasmid-dependent phages have been characterized. Here we systematically search for new plasmid-dependent phages using a targeted discovery platform, and find that they are in fact common and abundant in nature, and vastly unexplored in terms of their genetic diversity. Plasmid-dependent tectiviruses have highly conserved genetic architecture but show profound differences in their host range which do not reflect bacterial phylogeny. Finally, we show that plasmid-dependent tectiviruses are missed by metaviromic analyses, showing the continued importance of culture-based phage discovery. Taken together, these results indicate plasmid-dependent phages play an unappreciated evolutionary role in constraining horizontal gene transfer.

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Global prevalence, characteristics, and future prospects of IncX3 plasmids: A review

Cited by 27 publications

References 62 publications

Genomic characteristics of clinical carbapenem-resistant Escherichia coli isolates in a tertiary hospital, 2013–2021

Genomic characteristics of clinical carbapenem-resistant Escherichia coli isolates in a tertiary hospital, 2013–2021

Temperature effect on carbapenemase-encoding plasmid transfer

Diverse and abundant phages exploit conjugative plasmids

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