Global Spread and Molecular Characterization of CTX-M-Producing Salmonella Typhimurium Isolates

Guo, Lili; Zhao, Yongda

doi:10.3390/antibiotics10111417

Cited by 7 publications

(7 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…23 The main mechanisms underlying resistance to third-generation cephalosporins in Salmonella have been attributed to extended-spectrum β-lactamase (ESBL) genes obtained via horizontal transfer, and CTX-M enzymes include the most common ESBLs that are prevalent worldwide. 41 A previous study by our team showed that the rate of ESBL gene detection in our hospital was 16.38%, and CTX-M was the most common ESBL gene. 42 Our report suggested a continuous and slow increase in the rate of resistance to some antimicrobial agents, with only a significant increase in the rate of resistance to ciprofloxacin in 2013 because the CLSI lowered the ciprofloxacin susceptibility breakpoints in 2013, with the new MIC being applied only to Salmonella spp.…”

mentioning

confidence: 72%

Epidemiology, Serotype and Resistance of Salmonella Isolates from a Children’s Hospital in Hangzhou, Zhejiang, China, 2006–2021

Yue¹,

Liu²,

Li³

et al. 2022

IDR

View full text Add to dashboard Cite

This research investigated the dynamics of antibiotic resistance in Salmonella and the epidemiology of Salmonella infection in children. These data can aid in the prevention and control of the Salmonella epidemic and the diagnosis and treatment of salmonellosis. Methods: In this study, we retrospectively reviewed and analysed data regarding epidemiology, clinical symptoms, Salmonella serotypes, and antibiotic resistance from the medical records of patients with Salmonella infections in Hangzhou Children's Hospital from April 2006 to December 2021. Results: A total of 2099 Salmonella isolates were identified during the 16-year study period, and 98.6% (2069) of the isolates were isolated from stool. About 84.5% (1773/2099) of the total Salmonella isolates were detected from May to October. The median age of the 2099 children with Salmonella infection was 1.4 years (17 months) (IQR: 0.9-2.8 years). In 1572 (74.9%) patients, the course of the disease was limited to uncomplicated gastroenteritis. S. Typhimurium (805/2099, 38.4%) was predominant, followed by S. Enteritidis (290/2099, 13.8%). The total number of serotypes and the number of less common serotypes are increasing. Nontyphoid Salmonella that cause invasive infections, including S. Typhimurium, S. Stanley, and S. Choleraesuis, accounted for 60.0% (18/30). The Salmonella strains were resistant to ampicillin, ampicillin-sulbactam, trimethoprim-sulfamethoxazole, ceftriaxone, and ciprofloxacin at percentages of 71.5%, 51.5%, 36.5%, 22.4%, and 14.7%, respectively. No imipenem-resistant strains were identified. 24.8% of the isolates exhibited multidrug resistance (MDR). Conclusion: S. typhimurium and S. enteritidis were the dominant serotypes in children (<2 years) with salmonella-infected arrhoea in Hangzhou, China. Ongoing serotype monitoring should be necessitated and dynamic changes in serotypes should be carefully examined to prevent the sudden outbreak of foodborne illness. Salmonella exhibits a higher rate of resistance to common antibiotics, and the risk of multidrug resistance should not be ignored. Therefore, clinicians should administer antibiotics judiciously according to the results of drug sensitivity tests.

show abstract

mentioning

confidence: 72%

Epidemiology, Serotype and Resistance of Salmonella Isolates from a Children’s Hospital in Hangzhou, Zhejiang, China, 2006–2021

Yue¹,

Liu²,

Li³

et al. 2022

IDR

View full text Add to dashboard Cite

show abstract

“…variety of first-line drugs such as ampicillin, chloramphenicol and co-trimoxazole (5). Therefore, an appropriate molecular typing method should be used in combination with serotyping to investigate the epidemiology of Salmonella.…”

Section: Multi-locus Sequence and Drug Resistance Analysis Of Salmone...mentioning

confidence: 99%

“…Salmonella serotypes are typically associated with their host adaptation and virulence capability, rendering serotyping to be a key tool for Salmonella surveillance and outbreak investigations ( 4 ). However, treatment of invasive salmonellosis has been compromised due to the emergence of Salmonella strains that are resistant to a variety of first-line drugs such as ampicillin, chloramphenicol and co-trimoxazole ( 5 ). Therefore, an appropriate molecular typing method should be used in combination with serotyping to investigate the epidemiology of Salmonella .…”

Section: Introductionmentioning

confidence: 99%

Multi‑locus sequence and drug resistance analysis of Salmonella infection in children with diarrhea in Guangdong to identify the dominant ST and cause of antibiotic‑resistance

Tang

et al. 2022

Exp Ther Med

View full text Add to dashboard Cite

Multi-locus sequence typing (MLST) can be used to analyze the homology among the drug resistance gene cassettes in Salmonella and determine the prevalence. Information extracted using this technique can provide a theoretical basis for hospitals to devise protocols to control Salmonella infections. The aim of the present study was to investigate the possible association between drug resistance and integrons in clinical isolates of Salmonella from human fecal samples. Therefore, in the present study, 52 clinical fecal isolates of non-duplicate (i.e., not genome contamination) Salmonella were harvested from children with diarrhea and used for bacterial identification using biochemical tests, drug susceptibility analysis by antibiotic susceptibility testing and serotype identification using an agglutination assay. In total, seven Salmonella housekeeping genes (chorismate synthase, β sliding clamp of DNA polymerase III, uroporphyrinogen-III synthase, histidinol dehydrogenase, phosphoribosylaminoimidazole carboxylase catalytic subunit, 2-oxoglutarate dehydrogenase E1 component and homoserine dehydrogenase) were amplified and sequenced using MLST, before sequence alignment was performed against the Pub MLST database to determine the sequence-typed (ST) strains and construct genotypic evolutionary diagrams. Subsequently, the 52 Salmonella strains were subdivided into 11 serotypes and 11 sequence types. The dominant subtypes were found to be Salmonella typhimurium ST34 and ST19, which were diversely distributed. However, no new subtypes were found. Although the serotypes, including ST19, ST29, ST34, ST40, ST11, ST27, ST469, ST365, ST1499, ST413 and ST588, were closely associated with the MLST subtype, they did not correspond entirely. The detection rate of class I integrons was 38.46% (20/52), but no class II and III integrons were detected. The variable regions of three of 20 class I integrons were found to be amplified, whereas nine gene cassettes, including dihydrofolate reductase A12, open reading frame F, aminoglycoside-adenylyltransferase (aad)A2, aadA22, aadA23, aadA1, cadmium-translocating P-type ATPase 2, lincosamide and linF, were associated with drug resistance. These data suggest that Class I integrons are important factors underlying drug resistance in Salmonella, which may serve a role in the spread of drug resistance and warrant specific focus. In addition, MLST typing and serotyping should be applied cooperatively in epidemiological research.

show abstract

“…Therefore, the clinical importance of nasal carriage of CTX-M-producing E. coli might be underestimated. Although horizontal transfer is the main reason for bla CTX-M dissemination, clonal spread of bla CTX-M -harbouring strains, such as E. coli ST8369 in this study, CTX-M-15-producing E. coli ST949 in water surfaces, E. coli ST2179 encoding CTX-M-65 in retail meat, and bla CTX-M-transmission of bla CTX-M in E. coli and Salmonella from various sources (Chen et al, 2021;Guo and Zhao, 2021;Yang et al, 2014;Nadimpalli et al, 2019;Zhang et al, 2021;Zeng et al, 2021). In this study, various plasmids such as IncHI2, IncK1, IncX1, and IncI1 were associated with bla CTX-M .…”

Section: Discussionmentioning

confidence: 74%

“…Horizontal transfer mediated by plasmids and mobile elements is responsible for the global spread of bla CTX-M ( Bevan et al., 2017 ; Partridge et al., 2018 ). For example, IncI, IncFII, and IncHI2 plasmids facilitate the horizontal transmission of bla CTX-M in E. coli and Salmonella from various sources ( Chen et al., 2021 ; Guo and Zhao, 2021 ; Yang et al., 2014 ; Nadimpalli et al., 2019 ; Zhang et al., 2021 ; Zeng et al., 2021 ). In this study, various plasmids such as IncHI2, IncK1, IncX1, and IncI1 were associated with bla CTX-M .…”

Section: Discussionmentioning

confidence: 99%

Nasal carriage of CTX-M-55-producing Escherichia coli ST8369 in a healthy cohort in the city of Yangzhou, China

Wang

Jiang²,

Shao³

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

This study aimed to investigate the prevalence and diversity of extended-spectrum β-lactamases (ESBL)-producing Escherichia coli isolates from healthy individuals in a community and to elucidate their dissemination mechanism. Cefotaxime-resistant E. coli were isolated from 95 samples of healthy persons from one community in Yangzhou, China, and were tested for minimal inhibitory concentrations of 14 antimicrobial agents. The isolates were subjected to whole genome sequencing by Illumina Hiseq or PacBio single-molecule real-time sequencing. A total of 30 cefotaxime-resistant E. coli isolates were obtained, carrying blaCTX-M (n=29) or blaDHA (n=1), of which the blaCTX-M-55 (n=19) was the most predominant genotype. One novel blaCTX-M variant blaCTX-M-252 was identified. Thirteen CTX-M-55-producing E. coli isolates belonged to ST8369 from nasal (n=12) or faecal (n=1) samples shared the identical cgMLST type, resistance profiles, resistance genes, plasmid replicons, and a 5,053-bp blaCTX-M-55 structure ΔIS26-ΔISEcp1-blaCTX-M-55-Δorf477-ΔTn2. The blaCTX-M-55 gene was located on IncHI2/ST3 plasmid in E. coli ST8369. The lengths of blaCTX-M/blaDHA-carrying contigs in the remaining 17 E. coli strains ranged from 1,663 to 382,836 bp, located on chromosome (n=4) or plasmids (n=5); the location of the other eight contigs could not be determined due to incomplete assembly. The blaCTX-M was associated with ISEcp1 as previously reported. Nasal colonization of CTX-M-55-producing ST8369 E. coli strains has occurred among healthy individuals in one community. There is a potential risk of antimicrobial resistance dissemination between humans within one community through close contact or environment via aerosols or dust. Therefore, surveillance of nasal carriage of blaCTX-M in communities is warranted to further monitor the spread of the antimicrobial resistance genes in China.

show abstract

Global Spread and Molecular Characterization of CTX-M-Producing Salmonella Typhimurium Isolates

Cited by 7 publications

References 43 publications

Epidemiology, Serotype and Resistance of Salmonella Isolates from a Children’s Hospital in Hangzhou, Zhejiang, China, 2006–2021

Epidemiology, Serotype and Resistance of Salmonella Isolates from a Children’s Hospital in Hangzhou, Zhejiang, China, 2006–2021

Multi‑locus sequence and drug resistance analysis of Salmonella infection in children with diarrhea in Guangdong to identify the dominant ST and cause of antibiotic‑resistance

Nasal carriage of CTX-M-55-producing Escherichia coli ST8369 in a healthy cohort in the city of Yangzhou, China

Contact Info

Product

Resources

About