Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways

Duangjan, Chatrawee; Rangsinth, Panthakarn; Gu, Xiao-Jie; Zhang, Shaoxiong; Wink, Michael

doi:10.1016/j.phymed.2019.153061

Cited by 65 publications

(83 citation statements)

References 28 publications

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“…It has been found, for instance, that the treatment with myricetin [ 25 ] or leaf extract of Caesalpinia mimosoides [ 151 ] did not change the location of the SKN-1. However, the exposure to Glochidion zeylanicum leaf extract induced a significant translocation of SKN-1::GFP [ 153 ]. GST-4, an isoform of glutathione S-transferases regulated by the SKN-1 transcription factor was also explored.…”

Section: Methodological Approaches For Antioxidants Evaluation In mentioning

confidence: 99%

“…GST-4, an isoform of glutathione S-transferases regulated by the SKN-1 transcription factor was also explored. Using the transgenic strain Pgst-4::gfp , it was demonstrated that the treatment of worms with curcumin [ 135 ], epicatechin [ 21 ] or Glochidion zeylanicum leaf extract [ 153 ] increased GST-4 expression. However, no change in GST-4 expression was found for quercetin [ 22 ] or leaf extract of Caesalpinia mimosoides [ 151 ].…”

Section: Methodological Approaches For Antioxidants Evaluation In mentioning

confidence: 99%

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Caenorhabditis elegans as a Model Organism to Evaluate the Antioxidant Effects of Phytochemicals

et al. 2020

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The nematode Caenorhabditis elegans was introduced as a model organism in biological research by Sydney Brenner in the 1970s. Since then, it has been increasingly used for investigating processes such as ageing, oxidative stress, neurodegeneration, or inflammation, for which there is a high degree of homology between C. elegans and human pathways, so that the worm offers promising possibilities to study mechanisms of action and effects of phytochemicals of foods and plants. In this paper, the genes and pathways regulating oxidative stress in C. elegans are discussed, as well as the methodological approaches used for their evaluation in the worm. In particular, the following aspects are reviewed: the use of stress assays, determination of chemical and biochemical markers (e.g., ROS, carbonylated proteins, lipid peroxides or altered DNA), influence on gene expression and the employment of mutant worm strains, either carrying loss-of-function mutations or fluorescent reporters, such as the GFP.

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Section: Methodological Approaches For Antioxidants Evaluation In mentioning

confidence: 99%

Section: Methodological Approaches For Antioxidants Evaluation In mentioning

confidence: 99%

Caenorhabditis elegans as a Model Organism to Evaluate the Antioxidant Effects of Phytochemicals

et al. 2020

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“…Recent studies have demonstrated the possibility of providing the worms with plant extracts through two different methods. The first method consists in adding the extract to a liquid medium either in its solid form or dissolved in a solvent [26,27,40]. In some case, solvents such as Dimethyl sulfoxide (DMSO), were added to the medium to dissolve the plant extract.…”

Section: Determination Of the Optimal Parameters To Perform The Longementioning

confidence: 99%

“…The next step in this methodology would be to study the mechanism of action of the fractions by using nematodes mutants. Some signalling pathways have already been described to be involved in the mechanism of the longevity [35,40] and could represent a new study target.…”

Section: Beneficial Effects Of Cheese Fractions On C Elegans Lifespanmentioning

confidence: 99%

Development of an innovative methodology combining chemical fractionation and in vivo analysis to investigate the biological properties of cheese

et al. 2020

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With the ever-increasing human lifespan, age-related affections have become a public health issue. The health sector is looking for new bioactive compounds to respond to this demand. The unexplored microbial biodiversity and its metabolites represent a major source of innovative bioactive molecules with health potential. Fermented foods, such as raw-milk cheese, have already been investigated for their rich microbial environment, especially for their organoleptic qualities. But studies remain limited regarding their effects on health and few metabolites of microbial origin have been identified. An efficient methodology was developed in this study to investigate the biological effect of raw-milk cheese, combining a chemical fractionation, to isolate the most metabolites from the cheese matrix, and an in vivo biological test using Caenorhabditis elegans. C. elegans was brought into contact with cheese extracts, obtained by means of chemical fractionation, and with freeze-dried whole cheese by supplementing the nematode growth medium. A longevity assay was performed to evaluate the effects of the extracts on the worms. Our results demonstrate the feasibility of the method developed to bring the worms into contact of the cheese extracts. The evaluation of the effects of the extracts on the longevity was possible. Some extracts showed a beneficial effect as extract W70 for example, obtained with water, which increases the mean lifespan by 16% and extends the longevity by 73% (p < 0.0001).

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“…FOXO-signalling pathway and other pathways such as the peroxisome, mTOR, and AGE-RAGE-signalling pathway are key players in longevity (Li et al 2019). The antiageing property of Glochidion zeylanicum, an antioxidant-rich plant found in Thailand, is attributed to DAF-16/FOXO and SKN-1/Nrf-2 transcription factors (Duangjan et al 2019). The livers of aged rats and obese mice with insulin resistance showed elevated FOXO 6 levels .…”

Section: Foxo Transcription Factorsmentioning

confidence: 99%

Sleep, brain vascular health and ageing

et al. 2020

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Sleep maintains the function of the entire body through homeostasis. Chronic sleep deprivation (CSD) is a prime health concern in the modern world. Previous reports have shown that CSD has profound negative effects on brain vasculature at both the cellular and molecular levels, and that this is a major cause of cognitive dysfunction and early vascular ageing. However, correlations among sleep deprivation (SD), brain vascular changes and ageing have barely been looked into. This review attempts to correlate the alterations in the levels of major neurotransmitters (acetylcholine, adrenaline, GABA and glutamate) and signalling molecules (Sirt1, PGC1α, FOXO, P66 shc , PARP1) in SD and changes in brain vasculature, cognitive dysfunction and early ageing. It also aims to connect SD-induced loss in the number of dendritic spines and their effects on alterations in synaptic plasticity, cognitive disabilities and early vascular ageing based on data available in scientific literature. To the best of our knowledge, this is the first article providing a pathophysiological basis to link SD to brain vascular ageing.

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Glochidion zeylanicum leaf extracts exhibit lifespan extending and oxidative stress resistance properties in Caenorhabditis elegans via DAF-16/FoxO and SKN-1/Nrf-2 signaling pathways

Cited by 65 publications

References 28 publications

Caenorhabditis elegans as a Model Organism to Evaluate the Antioxidant Effects of Phytochemicals

Caenorhabditis elegans as a Model Organism to Evaluate the Antioxidant Effects of Phytochemicals

Development of an innovative methodology combining chemical fractionation and in vivo analysis to investigate the biological properties of cheese

Sleep, brain vascular health and ageing

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